The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Quantifying cell-generated mechanical forces within living embryonic tissues
Open accessAbstract
Cell-generated mechanical forces play a critical role during tissue morphogenesis and organ formation in the embryo. Little is known about how these forces shape embryonic organs, mainly because it has not been possible to measure cellular forces within developing three-dimensional (3D) tissues in vivo. We present a method to quantify cell-generated mechanical stresses exerted locally within living embryonic tissues, using fluorescent, cell-sized oil microdroplets with defined mechanical properties and coated with adhesion receptor ligands. After a droplet is introduced between cells in a tissue, local stresses are determined from droplet shape deformations, measured using fluorescence microscopy and computerized image analysis. Using this method, we quantified the anisotropic stresses generated by mammary epithelial cells cultured within 3D aggregates, and we confirmed that these stresses (3.4 nN μm−2) are dependent on myosin II activity and are more than twofold larger than stresses generated by cells of embryonic tooth mesenchyme, either within cultured aggregates or in developing whole mouse mandibles.
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Cites
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Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
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Walker, C., Mojares, E., & Del Río Hernández, A. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19103028 · Cited by 1,084
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Saw, T. B., Doostmohammadi, A., Nier, V., Kocgozlu, L., Thampi, S., Toyama, Y., Marcq, P., Lim, C. T., Yeomans, J. M., & Ladoux, B. (2017) · Nature · doi:10.1038/nature21718 · Cited by 831
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Ladoux, B., & Mège, R.-M. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.98 · Cited by 749
In vivo quantification of spatially varying mechanical properties in developing tissues
Serwane, F., Mongera, A., Rowghanian, P., Kealhofer, D. A., Lucio, A. A., Hockenbery, Z. M., & Campàs, O. (2017) · Nature Methods · doi:10.1038/nmeth.4101 · Cited by 367
Self-citationAdherens junctions as molecular regulators of emergent tissue mechanics
Campàs, O., Noordstra, I., & Yap, A. S. (2024) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-023-00688-7 · Cited by 149
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Metadata
- Authors
- Campàs, O., Mammoto, T., Hasso, S., Sperling, R. A., O'Connell, D., Bischof, A. G., Maas, R., Weitz, D. A., Mahadevan, L., & Ingber, D. E.
- Venue
- Nature Methods
- Published
- 2013-12-08
- Volume
- 11
- Issue
- 2
- Pages
- 183-189
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2097679878
- Cited by
- 479
- References
- 1
- FWCI
- 15.11
- Type
- journal article
- Language
- en
- Open access
- green
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- Campàs, O., Mammoto, T., Hasso, S., Sperling, R. A., O'Connell, D., Bischof, A. G., Maas, R., Weitz, D. A., Mahadevan, L., & Ingber, D. E. (2014). Quantifying cell-generated mechanical forces within living embryonic tissues. Nature Methods. https://doi.org/10.1038/nmeth.2761
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