The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein
Open accessAbstract
A method for non-invasive visualization of genetically labeled cells in animal disease models with micrometer-level resolution would greatly facilitate development of cell-based therapies. Imaging of fluorescent proteins (FPs) using red excitation light in the 'optical window' above 600 nm is one potential method for visualizing implanted cells. However, previous efforts to engineer FPs with peak excitation beyond 600 nm have resulted in undesirable reductions in brightness. Here we report three new red-excitable monomeric FPs obtained by structure-guided mutagenesis of mNeptune. Two of these, mNeptune2 and mNeptune2.5, demonstrate improved maturation and brighter fluorescence than mNeptune, whereas the third, mCardinal, has a red-shifted excitation spectrum without reduction in brightness. We show that mCardinal can be used to non-invasively and longitudinally visualize the differentiation of myoblasts into myocytes in living mice with high anatomical detail.
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Self-citationCell and tissue autofluorescence research and diagnostic applications
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Improving FRET dynamic range with bright green and red fluorescent proteins
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Self-citationEngineering a stem cell house into a home
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Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging
Shcherbakova, D. M., Baloban, M., Emelyanov, A. V., Brenowitz, M., Guo, P., & Verkhusha, V. V. (2016) · Nature Communications · doi:10.1038/ncomms12405 · Cited by 312
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Self-citationA highly photostable and bright green fluorescent protein
Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01278-2 · Cited by 293
Identification of the gene that codes for the σ 2 receptor
Alon, A., Schmidt, H. R., Wood, M. D., Sahn, J. J., Martin, S. F., & Kruse, A. C. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1705154114 · Cited by 267
Spatiotemporal regulation of liquid-like condensates in epigenetic inheritance
Wan, G., Fields, B. D., Spracklin, G., Shukla, A., Phillips, C. M., & Kennedy, S. (2018) · Nature · doi:10.1038/s41586-018-0132-0 · Cited by 207
Clayton, D. F., Anreiter, I., Aristizabal, M., Frankland, P. W., Binder, E. B., & Citri, A. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1820837116 · Cited by 56
Metadata
- Authors
- Chu, J., Haynes, R. D., Corbel, S. Y., Li, P., González-González, E., Burg, J. S., Ataie, N. J., Lam, A. J., Cranfill, P. J., Baird, M. A., Davidson, M. W., Ng, H.-L., Garcia, K. C., Contag, C. H., Shen, K., Blau, H. M., & Lin, M. Z.
- Venue
- Nature Methods
- Published
- 2014-03-16
- Volume
- 11
- Issue
- 5
- Pages
- 572-578
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2163017334
- Cited by
- 230
- References
- 8
- FWCI
- 12.29
- Type
- journal article
- Language
- en
- Open access
- green
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