The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
A microfluidic device for label-free, physical capture of circulating tumor cell clusters
Open accessAbstract
Cancer cells metastasize through the bloodstream either as single migratory circulating tumor cells (CTCs) or as multicellular groupings (CTC clusters). Existing technologies for CTC enrichment are designed to isolate single CTCs, and although CTC clusters are detectable in some cases, their true prevalence and significance remain to be determined. Here we developed a microchip technology (the Cluster-Chip) to capture CTC clusters independently of tumor-specific markers from unprocessed blood. CTC clusters are isolated through specialized bifurcating traps under low–shear stress conditions that preserve their integrity, and even two-cell clusters are captured efficiently. Using the Cluster-Chip, we identified CTC clusters in 30–40% of patients with metastatic breast or prostate cancer or with melanoma. RNA sequencing of CTC clusters confirmed their tumor origin and identified tissue-derived macrophages within the clusters. Efficient capture of CTC clusters will enable the detailed characterization of their biological properties and role in metastasis.
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Cites
PD-1 and PD-L1 Immune Checkpoint Blockade to Treat Breast Cancer
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Self-citationCirculating Breast Tumor Cells Exhibit Dynamic Changes in Epithelial and Mesenchymal Composition
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Self-citationCirculating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis
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Crosby, D., Bhatia, S., Brindle, K. M., Coussens, L. M., Dive, C., Emberton, M., Esener, S., Fitzgerald, R. C., Gambhir, S. S., Kuhn, P., et al. (2022) · Science · doi:10.1126/science.aay9040 · Cited by 1,379
Liquid biopsy and minimal residual disease - latest advances and implications for cure
Pantel, K., & Alix-Panabières, C. (2019) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-019-0187-3 · Cited by 1,110
Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment
Huang, G., Li, F., Zhao, X., Ma, Y., Li, Y., Lin, M., Jin, G., Lu, T. J., Genin, G. M., & Xu, F. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00094 · Cited by 825
Enabling Technologies for Personalized and Precision Medicine
Ho, D., Quake, S. R., McCabe, E. R. B., Chng, W. J., Chow, E. K., Ding, X., Gelb, B. D., Ginsburg, G. S., Hassenstab, J., Ho, C.-M., et al. (2020) · Trends in Biotechnology · doi:10.1016/j.tibtech.2019.12.021 · Cited by 517
Point-of-Care Diagnostics: Recent Developments in a Connected Age
Nayak, S., Blumenfeld, N. R., Laksanasopin, T., & Sia, S. K. (2017) · Analytical Chemistry · doi:10.1021/acs.analchem.6b04630 · Cited by 508
Metadata
- Authors
- Sarioglu, A. F., Aceto, N., Kojic, N., Donaldson, M. C., Zeinali, M., Hamza, B., Engstrom, A., Zhu, H., Sundaresan, T. K., Miyamoto, D. T., Luo, X., Bardia, A., Wittner, B. S., Ramaswamy, S., Shioda, T., Ting, D. T., Stott, S. L., Kapur, R., Maheswaran, S., Haber, D. A., & Toner, M.
- Venue
- Nature Methods
- Published
- 2015-05-18
- Volume
- 12
- Issue
- 7
- Pages
- 685-691
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W1835655851
- Cited by
- 709
- References
- 36
- FWCI
- 42.62
- Type
- journal article
- Language
- en
- Open access
- green
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- Sarioglu, A. F., Aceto, N., Kojic, N., Donaldson, M. C., Zeinali, M., Hamza, B., Engstrom, A., Zhu, H., Sundaresan, T. K., Miyamoto, D. T., Luo, X., Bardia, A., Wittner, B. S., Ramaswamy, S., Shioda, T., Ting, D. T., Stott, S. L., Kapur, R., Maheswaran, S., . . . Toner, M. (2015). A microfluidic device for label-free, physical capture of circulating tumor cell clusters. Nature Methods. https://doi.org/10.1038/nmeth.3404
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
