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LOVTRAP: an optogenetic system for photoinduced protein dissociation
Open accessAbstract
LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.
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PHENIX : a comprehensive Python-based system for macromolecular structure solution
Adams, P. D., Afonine, P. V., Bunkóczi, G., Chen, V. B., Davis, I. W., Echols, N., Headd, J. J., Hung, L.-W., Kapral, G. J., Grosse-Kunstleve, R. W., et al. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909052925 · Cited by 23,716
Phaser crystallographic software
McCoy, A. J., Grosse-Kunstleve, R. W., Adams, P. D., Winn, M. D., Storoni, L. C., & Read, R. J. (2007) · Journal of Applied Crystallography · doi:10.1107/s0021889807021206 · Cited by 20,612
Kabsch, W. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909047337 · Cited by 16,463
Towards automated crystallographic structure refinement with phenix.refine
Afonine, P. V., Grosse-Kunstleve, R. W., Echols, N., Headd, J. J., Moriarty, N. W., Mustyakimov, M., Terwilliger, T. C., Urzhumtsev, A., Zwart, P. H., & Adams, P. D. (2012) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444912001308 · Cited by 5,511
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ESCRT-mediated phagophore sealing during mitophagy
Zhen, Y., Spangenberg, H., Munson, M. J., Brech, A., Schink, K. O., Tan, K.-W., Sørensen, V., Wenzel, E. M., Radulovic, M., Engedal, N., et al. (2020) · Autophagy · doi:10.1080/15548627.2019.1639301 · Cited by 209
Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor
Yousefi, O. S., Günther, M., Hörner, M., Chalupsky, J., Wess, M., Brandl, S. M., Smith, R. W., Fleck, C., Kunkel, T., Zurbriggen, M. D., et al. (2019) · eLife · doi:10.7554/elife.42475 · Cited by 117
Photoactivatable CaMKII induces synaptic plasticity in single synapses
Shibata, A. C. E., Ueda, H. H., Eto, K., Onda, M., Sato, A., Ohba, T., Nabekura, J., & Murakoshi, H. (2021) · Nature Communications · doi:10.1038/s41467-021-21025-6 · Cited by 74
RhoA regulation in space and time
de Seze, J., Gatin, J., & Coppey, M. (2023) · FEBS Letters · doi:10.1002/1873-3468.14578 · Cited by 42
Nagpal, S., Swaminathan, K., Beaudet, D., Verdier, M., Wang, S., Berger, C. L., Berger, F., & Hendricks, A. G. (2024) · Cell Reports · doi:10.1016/j.celrep.2024.114649 · Cited by 13
Metadata
- Authors
- Wang, H., Vilela, M., Winkler, A., Tarnawski, M., Schlichting, I., Yumerefendi, H., Kuhlman, B., Liu, R., Danuser, G., & Hahn, K. M.
- Venue
- Nature Methods
- Published
- 2016-07-18
- Volume
- 13
- Issue
- 9
- Pages
- 755-758
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2487796353
- Cited by
- 311
- References
- 4
- FWCI
- 14.50
- Type
- journal article
- Language
- en
- Open access
- green
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- Wang, H., Vilela, M., Winkler, A., Tarnawski, M., Schlichting, I., Yumerefendi, H., Kuhlman, B., Liu, R., Danuser, G., & Hahn, K. M. (2016). LOVTRAP: an optogenetic system for photoinduced protein dissociation. Nature Methods. https://doi.org/10.1038/nmeth.3926
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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