The DOI matches its Crossref record; 88 of the 88 references it cites were found in a registry or an index.
Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor
Published version of a preprint
Abstract
The immune system distinguishes between self and foreign antigens. The kinetic proofreading (KPR) model proposes that T cells discriminate self from foreign ligands by the different ligand binding half-lives to the T cell receptor (TCR). It is challenging to test KPR as the available experimental systems fall short of only altering the binding half-lives and keeping other parameters of the interaction unchanged. We engineered an optogenetic system using the plant photoreceptor phytochrome B (PhyB) as a ligand to selectively control the dynamics of ligand binding to the TCR by light. This opto-ligand-TCR system was combined with the unique property of PhyB to continuously cycle between the binding and non-binding states under red light, with the light intensity determining the cycling rate and thus the binding duration. Mathematical modeling of our experimental datasets showed that indeed the ligand-TCR interaction half-life is the decisive factor for activating downstream TCR signaling, substantiating KPR.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Enzymatic assembly of DNA molecules up to several hundred kilobases
Gibson, D. G., Young, L., Chuang, R.-Y., Venter, J. C., Hutchison, C. A., & Smith, H. O. (2009) · Nature Methods · doi:10.1038/nmeth.1318 · Cited by 9,996
Phenotypic Analysis of Antigen-Specific T Lymphocytes
Altman, J. D., Moss, P. A. H., Goulder, P. J. R., Barouch, D. H., McHeyzer-Williams, M. G., Bell, J. I., McMichael, A. J., & Davis, M. M. (1996) · Science · doi:10.1126/science.274.5284.94 · Cited by 3,544 · cited 1×
“, 2007), tetrameric pMHC based on streptavidin are routinely used to stimulate the TCR (Altman et al., 1996) and to obtain insight into ligand discrimination by T cells (Stone et al.”
BackgroundHopfield, J. J. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.10.4135 · Cited by 1,594
Veillette, A., Bookman, M. A., Horak, E. M., & Bolen, J. B. (1988) · Cell · doi:10.1016/0092-8674(88)90053-0 · Cited by 1,435
PHYTOCHROME STRUCTURE AND SIGNALING MECHANISMS
Rockwell, N. C., Su, Y.-S., & Lagarias, J. C. (2006) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.56.032604.144208 · Cited by 1,088
The Principles of Engineering Immune Cells to Treat Cancer
Lim, W. A., & June, C. H. (2017) · Cell · doi:10.1016/j.cell.2017.01.016 · Cited by 1,080
Spatiotemporal control of cell signalling using a light-switchable protein interaction
Levskaya, A., Weiner, O. D., Lim, W. A., & Voigt, C. A. (2009) · Nature · doi:10.1038/nature08446 · Cited by 1,009
Kinetic proofreading in T-cell receptor signal transduction.
McKeithan, T. W. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.11.5042 · Cited by 955
LIGAND RECOGNITION BY αβ T CELL RECEPTORS
Davis, M. M., Boniface, J. J., Reich, Z., Lyons, D., Hampl, J., Arden, B., & Chien, Y.-H. (1998) · Annual Review of Immunology · doi:10.1146/annurev.immunol.16.1.523 · Cited by 924
Direct observation of ligand recognition by T cells
Irvine, D. J., Purbhoo, M. A., Krogsgaard, M., & Davis, M. M. (2002) · Nature · doi:10.1038/nature01076 · Cited by 820
Metadata
- Authors
- Yousefi, O. S., Günther, M., Hörner, M., Chalupsky, J., Wess, M., Brandl, S. M., Smith, R. W., Fleck, C., Kunkel, T., Zurbriggen, M. D., Höfer, T., Weber, W., & Schamel, W. W.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2019-04-05
- Volume
- 8
- ISSN
- 2050-084X
- Cited by
- 117
- References
- 88
- Language
- en
- Licence
- creativecommons.org/licenses/by/4.0/ (vor)
- creativecommons.org/licenses/by/4.0/ (am)
- creativecommons.org/licenses/by/4.0/ (tdm)
- Funders
- Deutsche Forschungsgemeinschaft (EXC2189, GSC-4, EXC294, EXC81, INST 39/899-1 FUGG)
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor. https://app.cochat.ai/papers/10.7554/elife.42475
- The paper
- Yousefi, O. S., Günther, M., Hörner, M., Chalupsky, J., Wess, M., Brandl, S. M., Smith, R. W., Fleck, C., Kunkel, T., Zurbriggen, M. D., Höfer, T., Weber, W., & Schamel, W. W. (2019). Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor. eLife. https://doi.org/10.7554/elife.42475
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
