The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
A general method to fine-tune fluorophores for live-cell and in vivo imaging
Open accessAbstract
Pushing the frontier of fluorescence microscopy requires the design of enhanced fluorophores with finely tuned properties. We recently discovered that incorporation of four-membered azetidine rings into classic fluorophore structures elicits substantial increases in brightness and photostability, resulting in the Janelia Fluor (JF) series of dyes. We refined and extended this strategy, finding that incorporation of 3-substituted azetidine groups allows rational tuning of the spectral and chemical properties of rhodamine dyes with unprecedented precision. This strategy allowed us to establish principles for fine-tuning the properties of fluorophores and to develop a palette of new fluorescent and fluorogenic labels with excitation ranging from blue to the far-red. Our results demonstrate the versatility of these new dyes in cells, tissues and animals.
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Cites
Fiji: an open-source platform for biological-image analysis
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2019 · Cited by 66,890
Ultrasensitive fluorescent proteins for imaging neuronal activity
Chen, T.-W., Wardill, T. J., Sun, Y., Pulver, S. R., Renninger, S. L., Baohan, A., Schreiter, E. R., Kerr, R. A., Orger, M. B., Jayaraman, V., et al. (2013) · Nature · doi:10.1038/nature12354 · Cited by 6,474
A multilevel multimodal circuit enhances action selection in drosophila
Ohyama, T., Schneider-Mizell, C. M., Fetter, R. D., Aleman, J. V., Franconville, R., Rivera-Alba, M., Mensh, B. D., Branson, K. M., Simpson, J. H., Truman, J. W., et al. (2015) · Nature · doi:10.1038/nature14297 · Cited by 502
Cited by
Deep learning for cellular image analysis
Moen, E., Bannon, D., Kudo, T., Graf, W., Covert, M., & Van Valen, D. (2019) · Nature Methods · doi:10.1038/s41592-019-0403-1 · Cited by 1,225
Wang, C., Chi, W., Qiao, Q., Tan, D., Xu, Z., & Liu, X. (2021) · Chemical Society Reviews · doi:10.1039/d1cs00239b · Cited by 710
Bright and photostable chemigenetic indicators for extended in vivo voltage imaging
Abdelfattah, A. S., Kawashima, T., Singh, A., Novak, O., Liu, H., Shuai, Y., Huang, Y.-C., Campagnola, L., Seeman, S. C., Yu, J., et al. (2019) · Science · doi:10.1126/science.aav6416 · Cited by 455
Self-citationA general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy
Wang, L., Tran, M., D’Este, E., Roberti, J., Koch, B., Xue, L., & Johnsson, K. (2020) · Nature Chemistry · doi:10.1038/s41557-019-0371-1 · Cited by 382
A Host-Produced Quorum-Sensing Autoinducer Controls a Phage Lysis-Lysogeny Decision
Silpe, J. E., & Bassler, B. L. (2019) · Cell · doi:10.1016/j.cell.2018.10.059 · Cited by 363
Metadata
- Authors
- Grimm, J. B., Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C., Patel, R., Lu, R., Macklin, J. J., Keller, P. J., Ji, N., & Lavis, L. D.
- Venue
- Nature Methods
- Published
- 2017-09-04
- Volume
- 14
- Issue
- 10
- Pages
- 987-994
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2605776475
- Cited by
- 665
- References
- 3
- FWCI
- 31.47
- Type
- journal article
- Language
- en
- Open access
- green
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