Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development
Abstract
In the developing CNS, subtypes of neurons and glial cells are generated according to a schedule that is defined by cell-intrinsic mechanisms that function at the progenitor-cell level. However, no critical molecular switch for the temporal specification of CNS progenitor cells has been identified. We found that chicken ovalbumin upstream promoter-transcription factor I and II (Coup-tfI and Coup-tfII, also known as Nr2f1 and Nr2f2) are required for the temporal specification of neural stem/progenitor cells (NSPCs), including their acquisition of gliogenic competence, as demonstrated by their responsiveness to gliogenic cytokines. COUP-TFI and II are transiently co-expressed in the ventricular zone of the early embryonic CNS. The double knockdown of Coup-tfI/II in embryonic stem cell (ESC)-derived NSPCs and the developing mouse forebrain caused sustained neurogenesis and the prolonged generation of early-born neurons. These findings reveal a part of the timer mechanisms for generating diverse types of neurons and glial cells during CNS development.
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Nr2f1 and Nr2f2 influence neuronal specification by regulating cell cycle dynamics, which may explain why cell cycle factors have been implicated in interneuron diversity.
“Nr2f1 and Nr2f2 influence neuronal specification by regulating cell cycle dynamics”
From Development and Functional Diversification of Cortical Interneurons (2018) · cites this paper as Naka et al., 2008
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References 49 · Cited by 243
- Authors
- Naka, H., Nakamura, S., Shimazaki, T., & Okano, H.
- Affiliations
- Keio University (JP)
- Corresponding author
- Shimazaki, Takuya
- Okano, Hideyuki
- Venue
- Nature Neuroscience
- Published
- 2008-08-24
- Volume
- 11
- Issue
- 9
- Pages
- 1014-1023
- ISSN
- 1097-6256 (ISSN-L)
- 1546-1726
- OpenAlex
- W1977879168
- Cited by
- 243
- References
- 49
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 11 · 2023: 14 · 2022: 12 · 2021: 12 · 2020: 11 · 2019: 14 · 2018: 14 · 2017: 11 · 2016: 12 · 2015: 11 · 2014: 15 · 2013: 25 · 2012: 17
- FWCI
- 5.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nuclear Receptors and Signaling
- Keywords
- COUP-TFI, NR2F1, NR2F2, transcriptional repressor, neural stem/progenitor cells, temporal specifications, neurogenesis, gliogenesis, central nervous system development, ventricular zone, embryonic stem cell-derived neurons, forebrain
- MeSH terms
- Animals, Bromodeoxyuridine, Cell Differentiation, Cells, Cultured, Central Nervous System, Embryo, Mammalian, Glial Fibrillary Acidic Protein, Mice, Inbred ICR, Mutation, Neuroglia, Neurons, Organ Culture Techniques, Stem Cells, Time Factors, Transfection, Tubulin, Cytokines, Gene Expression Regulation, Developmental, RNA, Small Interfering, Chromatin Immunoprecipitation, Green Fluorescent Proteins, Mice, COUP Transcription Factor II, COUP Transcription Factor I, Neurogenesis
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