The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Determination of the elastic moduli of thin samples and adherent cells using conical atomic force microscope tips
Open accessAbstract
The atomic force microscope can detect the mechanical fingerprints of normal and diseased cells at the single-cell level under physiological conditions. However, atomic force microscopy studies of cell mechanics are limited by the 'bottom effect' artefact that arises from the stiff substrates used to culture cells. Because cells adhered to substrates are very thin, this artefact makes cells appear stiffer than they really are. Here, we show an analytical correction that accounts for this artefact when conical tips are used for atomic force microscope measurements of thin samples. Our bottom effect cone correction (BECC) corrects the Sneddon's model, which is widely used to measure Young's modulus, E. Comparing the performance of BECC and Sneddon's model on thin polyacrylamide gels, we find that although Sneddon's model overestimates E, BECC yields E values that are thickness-independent and similar to those obtained on thick regions of the gel. The application of BECC to measurements on live adherent fibroblasts demonstrates a significant improvement on the estimation of their local mechanical properties.
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The stiffness of living tissues and its implications for tissue engineering
Guimarães, C. F., Gasperini, L., Marques, A. P., & Reis, R. L. (2020) · Nature Reviews Materials · doi:10.1038/s41578-019-0169-1 · Cited by 1,515
Investigating cell mechanics with atomic force microscopy
Haase, K., & Pelling, A. E. (2015) · Journal of The Royal Society Interface · doi:10.1098/rsif.2014.0970 · Cited by 396
Metadata
- Authors
- Gavara, N., & Chadwick, R. S.
- Venue
- Nature Nanotechnology
- Published
- 2012-09-30
- Volume
- 7
- Issue
- 11
- Pages
- 733-736
- ISSN
- 1748-3387 (ISSN-L)
- 1748-3395
- OpenAlex
- W2038007769
- Cited by
- 317
- References
- 21
- FWCI
- 10.30
- Type
- journal article
- Language
- en
- Open access
- green
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