Laser-capture microdissection

The sequence of the human genome

Venter, J. C., Adams, M. D., Myers, E. W., Li, P. W., Mural, R. J., Sutton, G. G., Smith, H. O., Yandell, M., Evans, C. A., Holt, R. A., et al. (2001) · Science · doi:10.1126/science.1058040 · Cited by 13,909

Laser Capture Microdissection

Emmert-Buck, M. R., Bonner, R. F., Smith, P. D., Chuaqui, R. F., Zhuang, Z., Goldstein, S. R., Weiss, R. A., & Liotta, L. A. (1996) · Science · doi:10.1126/science.274.5289.998 · Cited by 2,534

The microenvironment of the tumour–host interface

Liotta, L. A., & Kohn, E. C. (2001) · Nature · doi:10.1038/35077241 · Cited by 2,310

Positional Cloning of the Gene for Multiple Endocrine Neoplasia-Type 1

Chandrasekharappa, S. C., Guru, S. C., Manickam, P., Olufemi, S.-E., Collins, F. S., Emmert-Buck, M. R., Debelenko, L. V., Zhuang, Z., Lubensky, I. A., Liotta, L. A., et al. (1997) · Science · doi:10.1126/science.276.5311.404 · Cited by 2,082

Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front

Paweletz, C. P., Charboneau, L., Bichsel, V. E., Simone, N. L., Chen, T., Gillespie, J. W., Emmert-Buck, M. R., Roth, M. J., Petricoin III, E. F., & Liotta, L. A. (2001) · Oncogene · doi:10.1038/sj.onc.1204265 · Cited by 956

Gene Expression Patterns in Human Liver Cancers

Chen, X., Cheung, S. T., So, S., Fan, S. T., Barry, C., Higgins, J., Lai, K.-M., Ji, J., Dudoit, S., Ng, I. O. L., et al. (2002) · Molecular Biology of the Cell · doi:10.1091/mbc.02-02-0023 · Cited by 878

Laser Capture Microdissection: Molecular Analysis of Tissue

Bonner, R. F., Emmert-Buck, M., Cole, K., Pohida, T., Chuaqui, R., Goldstein, S., & Liotta, L. A. (1997) · Science · doi:10.1126/science.278.5342.1481 · Cited by 874

Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces

Imbeaud, S. (2005) · Nucleic Acids Research · doi:10.1093/nar/gni054 · Cited by 528

Laser-capture microdissection: opening the microscopic frontier to molecular analysis

Simone, N. L., Bonner, R. F., Gillespie, J. W., Emmert-Buck, M. R., & Liotta, L. A. (1998) · Trends in Genetics · doi:10.1016/s0168-9525(98)01489-9 · Cited by 458

Protein microarrays: Meeting analytical challenges for clinical applications

Liotta, L. A., Espina, V., Mehta, A. I., Calvert, V., Rosenblatt, K., Geho, D., Munson, P. J., Young, L., Wulfkuhle, J., & Petricoin, E. F. (2003) · Cancer Cell · doi:10.1016/s1535-6108(03)00086-2 · Cited by 446

Adipocyte-derived collagen VI affects early mammary tumor progression in vivo, demonstrating a critical interaction in the tumor/stroma microenvironment

Iyengar, P., Espina, V., Williams, T. W., Lin, Y., Berry, D., Jelicks, L. A., Lee, H., Temple, K., Graves, R., Pollard, J., et al. (2005) · Journal of Clinical Investigation · doi:10.1172/jci200523424 · Cited by 428

Laser-Capture Microdissection, a Tool for the Global Analysis of Gene Expression in Specific Plant Cell Types: Identification of Genes Expressed Differentially in Epidermal Cells or Vascular Tissues of Maize[W]

Nakazono, M., Qiu, F., Borsuk, L. A., & Schnable, P. S. (2003) · The Plant Cell · doi:10.1105/tpc.008102 · Cited by 405

In vivo gene expression profile analysis of human breast cancer progression.

Sgroi, D. C., Teng, S., ROBINSON, G. C., LeVangie, R., Hudson, J. R., & Elkahloun, A. G. (1999) · PubMed · Cited by 390

Immuno-LCM: Laser Capture Microdissection of Immunostained Frozen Sections for mRNA Analysis

Fend, F., Emmert-Buck, M. R., Chuaqui, R., Cole, K., Lee, J., Liotta, L. A., & Raffeld, M. (1999) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)65251-0 · Cited by 378

Use of Reverse Phase Protein Microarrays and Reference Standard Development for Molecular Network Analysis of Metastatic Ovarian Carcinoma

Sheehan, K. M., Calvert, V. S., Kay, E. W., Lu, Y., Fishman, D., Espina, V., Aquino, J., Speer, R., Araujo, R., Mills, G. B., et al. (2005) · Molecular & Cellular Proteomics · doi:10.1074/mcp.t500003-mcp200 · Cited by 306

Proteomic analysis and identification of new biomarkers and therapeutic targets for invasive ovarian cancer

Jones, M. B., Krutzsch, H., Shu, H., Zhao, Y., Liotta, L. A., Kohn, E. C., & Petricoin, E. F. (2002) · PROTEOMICS · doi:10.1002/1615-9861(200201)2:1<76::aid-prot76>3.0.co;2-o · Cited by 290

Evaluation of Non-Formalin Tissue Fixation for Molecular Profiling Studies

Gillespie, J. W., Best, C. J. M., Bichsel, V. E., Cole, K. A., Greenhut, S. F., Hewitt, S. M., Ahram, M., Gathright, Y. B., Merino, M. J., Strausberg, R. L., et al. (2002) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)64864-x · Cited by 284

Molecular portraits and the family tree of cancer

Chung, C. H., Bernard, P. S., & Perou, C. M. (2002) · Nature Genetics · doi:10.1038/ng1038 · Cited by 262

Proteomics of human breast ductal carcinoma in situ.

Wulfkuhle, J. D., Sgroi, D. C., Krutzsch, H. C., McLean, K. C., McGarvey, K. M., Knowlton, M., Chen, S., Shu, H., Şahin, A. A., Kurek, R., et al. (2002) · PubMed · Cited by 256

Signal pathway profiling of ovarian cancer from human tissue specimens using reverse‐phase protein microarrays

Wulfkuhle, J. D., Aquino, J. A., Calvert, V. S., Fishman, D. A., Coukos, G., Liotta, L. A., & Petricoin, E. F. (2003) · PROTEOMICS · doi:10.1002/pmic.200300591 · Cited by 221

Signal pathway profiling of prostate cancer using reverse phase protein arrays

Grubb, R. L., Calvert, V. S., Wulkuhle, J. D., Paweletz, C. P., Linehan, W. M., Phillips, J. L., Chuaqui, R., Valasco, A., Gillespie, J., Emmert‐Buck, M., et al. (2003) · PROTEOMICS · doi:10.1002/pmic.200300598 · Cited by 194

Accurate Qualitative and Quantitative Proteomic Analysis of Clinical Hepatocellular Carcinoma Using Laser Capture Microdissection Coupled with Isotope-coded Affinity Tag and Two-dimensional Liquid Chromatography Mass Spectrometry

Li, C., Hong, Y., Tan, Y.-X., Zhou, H., Ai, J.-H., Li, S.-J., Zhang, L., Xia, Q.-C., Wu, J.-R., Wang, H.-Y., et al. (2004) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m300133-mcp200 · Cited by 185

FaQR , Required for the Biosynthesis of the Strawberry Flavor Compound 4-Hydroxy-2,5-Dimethyl-3(2H)-Furanone, Encodes an Enone Oxidoreductase

Raab, T., Lopez-Raez, J. A., Klein, D., Caballero, J. L., Moyano, E., Schwab, W. G., & Muñoz‐Blanco, J. (2006) · The Plant Cell · doi:10.1105/tpc.105.039784 · Cited by 181

Mapping Molecular Networks Using Proteomics: A Vision for Patient-Tailored Combination Therapy

Petricoin, E. F., Bichsel, V. E., Calvert, V. S., Espina, V. A., Winters, M. E., Young, L., Belluco, C., Trock, B. J., Lippman, M. E., Fishman, D. A., et al. (2005) · Journal of Clinical Oncology · doi:10.1200/jco.2005.02.509 · Cited by 170

Reversing the Effects of Formalin Fixation with Citraconic Anhydride and Heat: A Universal Antigen Retrieval Method

Namimatsu, S., Ghazizadeh, M., & Sugisaki, Y. (2005) · Journal of Histochemistry & Cytochemistry · doi:10.1177/002215540505300102 · Cited by 160

Laser capture microdissection and microarray expression analysis of lung adenocarcinoma reveals tobacco smoking- and prognosis-related molecular profiles.

Miura, K., Bowman, E. D., Simon, R., Peng, A. C., Robles, A. I., Jones, R. T., Katagiri, T., He, P., Mizukami, H., Charboneau, L., et al. (2002) · PubMed · Cited by 158

Laser micromanipulation systems as universal tools in cellular and molecular biology and in medicine.

Schütze, K., Pösl, H., & Lahr, G. (1998) · PubMed · Cited by 156

Physiological Mechanisms of Tumor-Cell Invasion and Migration

Geho, D. H., Bandle, R., Clair, T., & Liotta, L. A. (2005) · Physiology · doi:10.1152/physiol.00009.2005 · Cited by 147

Sensitive Immunoassay of Tissue Cell Proteins Procured by Laser Capture Microdissection

Simone, N. L., Remaley, A. T., Charboneau, L., Petricoin, E. F., III, Glickman, J., Emmert‐Buck, M. R., Fleisher, T. A., & Liotta, L. A. (2000) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)64749-9 · Cited by 136

Laser Capture Microdissection of Single Cells from Complex Tissues

Suarez‐Quian, C. A., Goldstein, S. R., Pohida, T. J., Smith, P. D., Peterson, J. I., Wellner, E., Ghany, M. G., & Bonner, R. F. (1999) · BioTechniques · doi:10.2144/99262rr03 · Cited by 129

Proteomic Approaches to the Diagnosis, Treatment, and Monitoring of Cancer

Wulfkuhle, J. D., Paweletz, C. P., Steeg, P. S., Petricoin, E. F., & Liotta, L. A. (2003) · Advances in experimental medicine and biology · doi:10.1007/978-1-4615-0081-0_7 · Cited by 126

Characterization of vascular protein expression patterns in cerebral ischemia/reperfusion using laser capture microdissection and ICAT‐nanoLC‐MS/MS

Haqqani, A. S., Nesic, M., Preston, E., Baumann, E., Kelly, J. F., & Stanimirovic, D. B. (2005) · The FASEB Journal · doi:10.1096/fj.05-3793com · Cited by 126

Genetic mosaic analysis based on Cre recombinase and navigated laser capture microdissection

Wong, M. H., Saam, J. R., Stappenbeck, T. S., Rexer, C. H., & Gordon, J. I. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.230237997 · Cited by 121

New technologies for biomarker analysis of prostate cancer progression: Laser capture microdissection and tissue proteomics

Paweletz, C. P., Liotta, L. A., & Petricoin, E. F. (2001) · Urology · doi:10.1016/s0090-4295(00)00964-x · Cited by 120

Liquid Tissue™: Proteomic Profiling of Formalin-Fixed Tissues

Prieto, D. A., Hood, B. L., Darfler, M. M., Guiel, T. G., Lucas, D. A., Conrads, T. P., Veenstra, T. D., & Krizman, D. B. (2005) · BioTechniques · doi:10.2144/05386su06 · Cited by 116

Proteomic Analysis of Apoptotic Pathways Reveals Prognostic Factors in Follicular Lymphoma

Gulmann, C., Espina, V. A., Petricoin, E. F., Longo, D. L., Santi, M., Knutsen, T., Raffeld, M. A., Jaffe, E. S., Liotta, L. A., & Feldman, A. L. (2005) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-05-0637 · Cited by 109

Molecular Profiling of Clinical Tissue Specimens

Emmert‐Buck, M. R., Strausberg, R. L., Krizman, D. B., Bonaldo, M. F., Bonner, R. F., Bostwick, D. G., Brown, M. R., Buetow, K. H., Chuaqui, R. F., Cole, K. A., et al. (2000) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)64979-6 · Cited by 93

IF-LCM: Laser capture microdissection of immunofluorescently defined cells for mRNA analysis

Murakami, H., Liotta, L. A., & Star, R. A. (2000) · Kidney International · doi:10.1046/j.1523-1755.2000.00295.x · Cited by 91

Laser capture microdissection: beyond functional genomics to proteomics.

Simone, N. L., Paweletz, C. P., Charboneau, L., et al. (2000) · PubMed · doi:10.1007/bf03262091 · Cited by 84

Proteome-wide analysis of head and neck squamous cell carcinomas using laser-capture microdissection and tandem mass spectrometry

Baker, H., Patel, V., Molinolo, A., et al. (2005) · Oral Oncology · doi:10.1016/j.oraloncology.2004.08.009 · Cited by 83

Laser Capture Microdissection: Beyond Functional Genomics to Proteomics

Simone, N. L., Paweletz, C. P., Charboneau, L., et al. (2000) · Molecular Diagnosis · doi:10.2165/00066982-200005040-00008 · Cited by 77

‘The 39 steps’ in gene expression profiling: critical issues and proposed best practices for microarray experiments

Imbeaud, S., & Auffray, C. (2005) · Drug Discovery Today · doi:10.1016/s1359-6446(05)03565-8 · Cited by 70

Transcriptome analysis of the ischemia-reperfused remodeling myocardium: temporal changes in inflammation and extracellular matrix

Roy, S., Khanna, S., Kuhn, D. E., Rink, C. L., Williams, W. T., Zweier, J. L., & Sen, C. K. (2006) · Physiological Genomics · doi:10.1152/physiolgenomics.00013.2006 · Cited by 68

Proteome analysis of hepatocellular carcinoma by laser capture microdissection

Ai, J.-H., Tan, Y., Ying, W., Hong, Y., Liu, S., Wu, M., Qian, X., & Wang, H. (2005) · PROTEOMICS · doi:10.1002/pmic.200500257 · Cited by 67

Genomics and proteomics: application of novel technology to early detection and prevention of cancer

Michener, C. M., Ardekani, A. M., Petricoin, E. F., III, Liotta, L. A., & Kohn, E. C. (2002) · Cancer Detection and Prevention · doi:10.1016/s0361-090x(02)00092-2 · Cited by 67

Development of rapid staining protocols for laser-capture microdissection of brain vessels from human and rat coupled to gene expression analyses

Mojsilovic‐Petrovic, J., Nesic, M., Pen, A., Zhang, W., & Stanimirovic, D. B. (2003) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2003.09.026 · Cited by 66

Cut out or poke in—the key to the world of single genes: laser micromanipulation as a valuable tool on the look-out for the origin of disease

Schütze, K., Becker, I., Becker, K., Thalhammer, S., Stark, R. W., Heckl, W. M., Böhm, M., & Pösl, H. (1997) · Genetic Analysis Biomolecular Engineering · doi:10.1016/s1050-3862(96)00169-6 · Cited by 65

Use of immuno-LCM to identify the in situ expression profile of cellular constituents of the tumor microenvironment

Buckanovich, R. J., Sasaroli, D., O’Brien-Jenkins, A., Botbyl, J. D., Conejo-Garcia, J. R., Benencia, F., Liotta, L. A., Gimotty, P. A., & Coukos, G. (2006) · Cancer Biology & Therapy · doi:10.4161/cbt.5.6.2676 · Cited by 59

Expression Microdissection

Tangrea, M. A., Chuaqui, R. F., Gillespie, J. W., Ahram, M. A., Gannot, G., Wallis, B. S., Best, C. J. M., Linehan, W. M., Liotta, L. A., Pohida, T. J., et al. (2004) · Diagnostic Molecular Pathology · doi:10.1097/01.pdm.0000135964.31459.bb · Cited by 55

Using DSP, a reversible cross-linker, to fix tissue sections for immunostaining, microdissection and expression profiling

Xiang, C. C., Mezey, E., Chen, M., Key, S., Ma, L., & Brownstein, M. J. (2004) · Nucleic Acids Research · doi:10.1093/nar/gnh185 · Cited by 54

Detection of Fusion Gene Transcripts in Fresh-Frozen and Formalin-Fixed Paraffin-Embedded Tissue Sections of Soft-Tissue Sarcomas After Laser Capture Microdissection and RT-PCR

Jin, L. G., Majerus, J. A., Oliveira, A. M., Inwards, C. Y., Nascimento, A. G., Burgart, L. J., & Lloyd, R. V. (2003) · Diagnostic Molecular Pathology · doi:10.1097/00019606-200312000-00006 · Cited by 53

Detection and identification of heat shock protein 10 as a biomarker in colorectal cancer by protein profiling

Melle, C., Bogumil, R., Ernst, G., Schimmel, B., Bleul, A., & von Eggeling, F. (2006) · PROTEOMICS · doi:10.1002/pmic.200500427 · Cited by 49

Selective capture of endothelial and perivascular cells from brain microvessels using laser capture microdissection

Kinnecom, K., & Pachter, J. S. (2005) · Brain Research Protocols · doi:10.1016/j.brainresprot.2005.08.002 · Cited by 48

Allelic Loss on Chromosome Arm 8p: Analysis of Sporadic Epithelial Ovarian Tumors

Brown, M. R., Chuaqui, R. F., Vocke, C. D., Berchuck, A., Middleton, L. P., Emmert‐Buck, M. R., & Kohn, E. C. (1999) · Gynecologic Oncology · doi:10.1006/gyno.1999.5439 · Cited by 48

Laser capture microdissection and single-cell RT-PCR without RNA purification

Keays, K. M., Owens, G. P., Ritchie, A. M., Gilden, D. H., & Burgoon, M. P. (2005) · Journal of Immunological Methods · doi:10.1016/j.jim.2005.04.018 · Cited by 44

Laser Capture Microdissection–Guided Fluorescence In Situ Hybridization and Flow Cytometric Cell Cycle Analysis of Purified Nuclei from Paraffin Sections

Difrancesco, L., Murthy, S. K., Luider, J. M., & Demetrick, D. J. (2000) · Modern Pathology · doi:10.1038/modpathol.3880120 · Cited by 43

Kinase substrate protein microarray analysis of human colon cancer and hepatic metastasis

Belluco, C., Mammano, E., Petricoin, E. F., Prevedello, L., Calvert, V. S., Liotta, L. A., Nitti, D., & Lise, M. (2005) · Clinica Chimica Acta · doi:10.1016/j.cccn.2005.03.024 · Cited by 40

The role of the extracellular matrix in tumor cell metastasis.

Stracke, M. L., Murata, J., Aznavoorian, S. A., & Liotta, L. A. (1994) · PubMed · Cited by 40

Microarray analysis of fluoro-gold labeled rat dopamine neurons harvested by laser capture microdissection

Yao, F., Yu, F., Gong, L., Taube, D., Rao, D. D., & MacKenzie, R. G. (2004) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2004.09.023 · Cited by 36

Laser Capture Microdissection of Epithelial Cancers Guided by Antibodies Against Fibroblast Activation Protein and Endosialin

Rupp, C., Dolznig, H., Puri, C., Schweifer, N., Sommergruber, W., Kraut, N., Rettig, W. J., Kerjaschki, D., & Garin‐Chesa, P. (2006) · Diagnostic Molecular Pathology · doi:10.1097/00019606-200603000-00006 · Cited by 35

Laser-microdissection for cell type- and compartment-specific analyses on genomic and proteomic level

Fink, L., Kwapiszewska, G., Wilhelm, J., et al. (2006) · Experimental and Toxicologic Pathology · doi:10.1016/j.etp.2006.02.010 · Cited by 34

Western blot analysis of a limited number of cells: a valuable adjunct to proteome analysis of paraffin wax‐embedded, alcohol‐fixed tissue after laser capture microdissection

Martinet, W., Abbeloos, V., Acker, N. V., Meyer, G. R. Y. D., Herman, A. G., & Kockx, M. M. (2004) · The Journal of Pathology · doi:10.1002/path.1525 · Cited by 33

Requirement of Cell Interactions through Adhesion Molecules in the Early Phase of T Cell Development

Wada, K., Kina, T., Kawamoto, H., Kondo, M., & Katsura, Y. (1996) · Cellular Immunology · doi:10.1006/cimm.1996.0128 · Cited by 26

Peptide patterns of laser dissected human trophoblasts analyzed by matrix‐assisted laser desorption/ionisation‐time of flight mass spectrometry

de Groot, C. J. M., Steegers‐Theunissen, R. P. M., Güzel, C., Steegers, E. A. P., & Luider, T. M. (2005) · PROTEOMICS · doi:10.1002/pmic.200400974 · Cited by 25

The use of laser capture microdissection (LCM) and quantitative polymerase chain reaction to define thyroid hormone receptor expression in human ‘term’ placenta

Chan, S., Murray, P., Franklyn, J. A., McCabe, C. J., & Kilby, M. D. (2004) · Placenta · doi:10.1016/j.placenta.2004.02.007 · Cited by 23

Rhodamine-RCA in vivo labeling guided laser capture microdissection of cancer functional angiogenic vessels in a murine squamous cell carcinoma mouse model.

Hunter, F., Xie, J., Trimble, C., Bur, M., & Li, K. C. (2006) · Molecular Cancer · doi:10.1186/1476-4598-5-5 · Cited by 21

Loss of p16 INK4A expression is associated with allelic imbalance/loss of heterozygosity of chromosome 9p21 in microdissected synovial sarcomas

Sabah, M., Cummins, R., Leader, M., & Kay, E. W. (2005) · Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin · doi:10.1007/s00428-005-0024-1 · Cited by 10

CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling

Platt, R. J., Chen, S., Zhou, Y., Yim, M. J., Swiech, L., Kempton, H. R., Dahlman, J. E., Parnas, O., Eisenhaure, T. M., Jovanovic, M., et al. (2014) · Cell · doi:10.1016/j.cell.2014.09.014 · Cited by 2,001

Single-cell RNA-seq: advances and future challenges

Saliba, A.-E., Westermann, A. J., Gorski, S. A., & Vogel, J. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku555 · Cited by 847

Spatially resolved transcriptomics and beyond

Crosetto, N., Bienko, M., & van Oudenaarden, A. (2015) · Nature Reviews Genetics · doi:10.1038/nrg3832 · Cited by 509

High-throughput mapping of single-neuron projections by sequencing of barcoded RNA

Kebschull, J. M., Garcia da Silva, P., Reid, A. P., Peikon, I. D., Albeanu, D. F., & Zador, A. M. (2016) · Neuron · doi:10.1016/j.neuron.2016.07.036 · Cited by 376

The emerging landscape of spatial profiling technologies

Moffitt, J. R., Lundberg, E., & Heyn, H. (2022) · Nature Reviews Genetics · doi:10.1038/s41576-022-00515-3 · Cited by 419