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Cited by 142

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Changing medium and passaging cell lines

Masters, John R, Stacey, Glyn N·2007·Nature Protocols
DOI 10.1038/nprot.2007.319
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Abstract

Cell lines are widely used in biomedical research. This protocol describes the methods used routinely to change the medium and passage the cells. Medium changes keep the cells healthy by providing fresh nutrients, while cell passage or splitting is required to maintain cells in exponential growth. Despite the simplicity of the methods used, each cell line has idiosyncracies. Whether working with one or several cell lines, there is no substitute for knowledge of their needs, including the range of phenotypes and growth patterns under different physical and nutrient conditions. Given the necessary care and attention, most cell lines are easy to maintain and grow.

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Crossref · 10.1038/nprot.2007.319

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Citations

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Research works printed but not linked: 8

  1. Schaeffer, W.I. Usage of vertebrate, invertebrate and plant cell, tissue and organ culture terminology. In Vitro 20, 19–24 (1984). · DOI
  2. Freshney, R.I. (ed.) Culture of Animal Cells 4th edn. (Wiley Liss, New York, 2000).
  3. Southam, C.M. Homotransplantation of human cell lines. Bull NY Acad. Med. 34, 416–423 (1958).
  4. Gugel, E.A. & Sanders, M.E. Needle-stick transmission of human colonic adenocarcinoma. N. Engl. J. Med. 315, 1487 (1986). · DOI
  5. Lloyd, G. & Jones, N. Infection of laboratory workers with hantavirus acquired from immunocytomas propagated in laboratory rats. J. Infect. 12, 117–125 (1984). · DOI
  6. Tedder, R.S. et al. Hepatitis B transmission from a contaminated cryopreservation tank. Lancet 346, 137–140 (1995). · DOI
  7. Hummeler, K., Davidson, W.L., Henle, W., LaBoccetta, A.C. & Ruch, H.G. Encephalomyelitis due to infection with Herpesvirus simiae (Herpes B virus); a report of two fatal, laboratory-acquired cases. N. Engl. J. Med. 261, 64–68 (1959). · DOI
  8. UKCCCR. UKCCCR guidelines for the use of cell lines in cancer research. Br. J. Cancer 82, 1495–1509 (2000). · DOI

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Metadata

Authors
Masters, J. R., & Stacey, G. N.
Venue
Nature Protocols
Publisher
Springer Science and Business Media LLC
Published
2007-09
Volume
2
Issue
9
Pages
2276-2284
ISSN
  • 1754-2189
  • 1750-2799
DOI
10.1038/nprot.2007.319
Cited by
142
References
1
Language
en
Licence
www.springer.com/tdm (tdm)

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