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Electrical stimulation systems for cardiac tissue engineering
Open accessAbstract
We describe a protocol for tissue engineering of synchronously contractile cardiac constructs by culturing cardiac cells with the application of pulsatile electrical fields designed to mimic those present in the native heart. Tissue culture is conducted in a customized chamber built to allow for cultivation of (i) engineered three-dimensional (3D) cardiac tissue constructs, (ii) cell monolayers on flat substrates or (iii) cells on patterned substrates. This also allows for analysis of the individual and interactive effects of pulsatile electrical field stimulation and substrate topography on cell differentiation and assembly. The protocol is designed to allow for delivery of predictable electrical field stimuli to cells, monitoring environmental parameters, and assessment of cell and tissue responses. The duration of the protocol is 5 d for two-dimensional cultures and 10 d for 3D cultures.
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Cited by
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Shin, S. R., Jung, S. M., Zalabany, M., Kim, K., Zorlutuna, P., Kim, S. B., Nikkhah, M., Khabiry, M., Azize, M., Kong, J., et al. (2013) · ACS Nano · doi:10.1021/nn305559j · Cited by 946
Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment
Huang, G., Li, F., Zhao, X., Ma, Y., Li, Y., Lin, M., Jin, G., Lu, T. J., Genin, G. M., & Xu, F. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00094 · Cited by 825
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Self-citationKim, B. S., Das, S., Jang, J., & Cho, D.-W. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.9b00808 · Cited by 465
Metadata
- Authors
- Tandon, N., Cannizzaro, C., Chao, P.-H. G., Maidhof, R., Marsano, A., Au, H. T. H., Radisic, M., & Vunjak-Novakovic, G.
- Venue
- Nature Protocols
- Published
- 2009-01-22
- Volume
- 4
- Issue
- 2
- Pages
- 155-173
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2158442971
- Cited by
- 528
- References
- 0
- FWCI
- 27.37
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Tandon, N., Cannizzaro, C., Chao, P.-H. G., Maidhof, R., Marsano, A., Au, H. T. H., Radisic, M., & Vunjak-Novakovic, G. (2009). Electrical stimulation systems for cardiac tissue engineering. Nature Protocols. https://doi.org/10.1038/nprot.2008.183
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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