mRNA expression analysis by real-time PCR
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The Ct value of each gene was normalized with β-actin.
“The Ct value of each gene was normalized with β-actin [27].”
From Intervention by picroside II on FFAs induced lipid accumulation and lipotoxicity in HepG2 cells (2021) · cites this paper as [27]
The Introduction specifies 18S RNA, not β-actin, as the endogenous control used to adjust target-gene expression for differences in template amount. The indexed copy provides no page number for this passage. (LLM, conf=0.90)
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Dhami-Shah, H., Vaidya, R., Udipi, S., Raghavan, S., Abhijit, S., Mohan, V., Balasubramanyam, M., & Vaidya, A. (2018) · Clinical and Molecular Hepatology · doi:10.3350/cmh.2017.0039 · Cited by 36 · see citing works
Intervention by picroside II on FFAs induced lipid accumulation and lipotoxicity in HepG2 cells
Dhami-Shah, H., Vaidya, R., Talwadekar, M., Shaw, E., Udipi, S., Kolthur-Seetharam, U., & Vaidya, A. D. B. (2021) · Journal of Ayurveda and Integrative Medicine · doi:10.1016/j.jaim.2021.04.007 · Cited by 15 · see citing works
“The Ct value of each gene was normalized with b-actin [27].”
Method
- Authors
- Zanoni, I., & Ostuni, R.
- Affiliations
- University of Milano-Bicocca (IT)
- Venue
- Protocol Exchange
- Published
- 2009-06-18
- ISSN
- 2043-0116 (ISSN-L)
- OpenAlex
- W2159878818
- Cited by
- 2
- References
- 0
- Citations by year
- 2021: 1 · 2017: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Protocol Exchange (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- mRNA expression, gene expression analysis, real-time PCR, quantitative gene expression
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