The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Conditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinase
Open accessAbstract
The development of animal models of lung cancer is critical to our understanding and treatment of the human disease. Conditional mouse models provide new opportunities for testing novel chemopreventatives, therapeutics and screening methods that are not possible with cultured cell lines or xenograft models. This protocol describes how to initiate tumors in two conditional genetic models of human non-small cell lung cancer (NSCLC) using the activation of oncogenic K-ras alone or in combination with the loss of function of p53. We discuss methods for sporadic expression of Cre in the lungs through engineered adenovirus or lentivirus, and provide a detailed protocol for the administration of the virus by intranasal inhalation or intratracheal intubation. The protocol requires 1–5 min per mouse with an additional 30–45 min to set-up and allow for the recovery of mice from anesthesia. Mice may be analyzed for tumor formation and progression starting 2–3 weeks after infection.
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In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector
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Generalized lacZ expression with the ROSA26 Cre reporter strain
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Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras
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Self-citationLung cancer in never smokers — a different disease
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Lentivirus-delivered stable gene silencing by RNAi in primary cells
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Somatic activation of the K-ras oncogene causes early onset lung cancer in mice
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CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
Platt, R. J., Chen, S., Zhou, Y., Yim, M. J., Swiech, L., Kempton, H. R., Dahlman, J. E., Parnas, O., Eisenhaure, T. M., Jovanovic, M., et al. (2014) · Cell · doi:10.1016/j.cell.2014.09.014 · Cited by 2,001
Commensal Microbiota Promote Lung Cancer Development via γδ T Cells
Jin, C., Lagoudas, G. K., Zhao, C., Bullman, S., Bhutkar, A., Hu, B., Ameh, S., Sandel, D., Liang, X. S., Mazzilli, S., et al. (2019) · Cell · doi:10.1016/j.cell.2018.12.040 · Cited by 964
Self-citationNFS1 undergoes positive selection in lung tumours and protects cells from ferroptosis
Alvarez, S. W., Sviderskiy, V. O., Terzi, E. M., Papagiannakopoulos, T., Moreira, A. L., Adams, S., Sabatini, D. M., Birsoy, K., & Possemato, R. (2017) · Nature · doi:10.1038/nature24637 · Cited by 787
Environment impacts the metabolic dependencies of Ras-driven non-small cell lung cancer
Davidson, S. M., Papagiannakopoulos, T., Olenchock, B. A., Heyman, J. E., Keibler, M. A., Luengo, A., Bauer, M. R., Jha, A. K., O’Brien, J. P., Pierce, K. A., et al. (2016) · Cell Metabolism · doi:10.1016/j.cmet.2016.01.007 · Cited by 737
Self-citationKRAS and YAP1 Converge to Regulate EMT and Tumor Survival
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Metadata
- Authors
- DuPage, M., Dooley, A. L., & Jacks, T.
- Venue
- Nature Protocols
- Published
- 2009-06-25
- Volume
- 4
- Issue
- 7
- Pages
- 1064-1072
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2027146979
- Cited by
- 894
- References
- 31
- FWCI
- 8.38
- Type
- journal article
- Language
- en
- Open access
- green
- Licence
- www.springer.com/tdm (tdm)
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