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Cited by 411

The DOI matches its Crossref record; 77 of the 77 references it cites were found in a registry or an index.

Preparation and incubation of precision-cut liver and intestinal slices for application in drug metabolism and toxicity studies

Open access
de Graaf, Inge A M, Olinga, Peter, de Jager, Marina H, Merema, Marjolijn T, de Kanter, Ruben, van de Kerkhof, Esther G, Groothuis, Geny M M·2010·Nature Protocols
DOI 10.1038/nprot.2010.111
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Abstract

Precision-cut tissue slices (PCTS) are viable ex vivo explants of tissue with a reproducible, well defined thickness. They represent a mini-model of the organ under study and contain all cells of the tissue in their natural environment, leaving intercellular and cell-matrix interactions intact, and are therefore highly appropriate for studying multicellular processes. PCTS are mainly used to study the metabolism and toxicity of xenobiotics, but they are suitable for many other purposes. Here we describe the protocols to prepare and incubate rat and human liver and intestinal slices. Slices are prepared from fresh liver by making a cylindrical core using a drill with a hollow bit, from which slices are cut with a specially designed tissue slicer. Intestinal tissue is embedded in cylinders of agarose before slicing. Slices remain viable for 24 h (intestine) and up to 96 h (liver) when incubated in 6- or 12-well plates under 95% O(2)/5% CO(2) atmosphere.

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Metadata

Authors
de Graaf, I. A. M., Olinga, P., de Jager, M. H., Merema, M. T., de Kanter, R., van de Kerkhof, E. G., & Groothuis, G. M. M.
Venue
Nature Protocols
Publisher
Springer Science and Business Media LLC
Published
2010-08-19
Volume
5
Issue
9
Pages
1540-1551
ISSN
  • 1750-2799 (ISSN-L)
  • 1754-2189
DOI
10.1038/nprot.2010.111
OpenAlex
W1979086975
Cited by
411
References
77
FWCI
10.71
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Preparation and incubation of precision-cut liver and intestinal slices for application in drug metabolism and toxicity studies. https://app.cochat.ai/papers/10.1038/nprot.2010.111
The paper
de Graaf, I. A. M., Olinga, P., de Jager, M. H., Merema, M. T., de Kanter, R., van de Kerkhof, E. G., & Groothuis, G. M. M. (2010). Preparation and incubation of precision-cut liver and intestinal slices for application in drug metabolism and toxicity studies. Nature Protocols. https://doi.org/10.1038/nprot.2010.111

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.