The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
The INTACT method for cell type–specific gene expression and chromatin profiling in Arabidopsis thaliana
Open accessAbstract
Genomic studies of cell differentiation and function within a whole organism depend on the ability to isolate specific cell types from a tissue, but this is technically difficult. We developed a method called INTACT (isolation of nuclei tagged in specific cell types) that allows affinity-based isolation of nuclei from individual cell types of a tissue, thereby circumventing the problems associated with mechanical purification techniques. In this method nuclei are affinity-labeled through transgenic expression of a biotinylated nuclear envelope protein in the cell type of interest. Total nuclei are isolated from transgenic plants and biotin-labeled nuclei are then purified using streptavidin-coated magnetic beads, without the need for specialized equipment. INTACT gives high yield and purity of nuclei from the desired cell types, which can be used for genome-wide analysis of gene expression and chromatin features. The entire procedure, from nuclei purification through cDNA preparation or chromatin immunoprecipitation (ChIP), can be completed within 2 d. The protocol we present assumes that transgenic lines are already available, and includes procedural details for amplification of cDNA or ChIP DNA prior to microarray or deep sequencing analysis.
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Rapid Flow Cytometric Analysis of the Cell Cycle in Intact Plant Tissues
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Self-citationProfiling histone modification patterns in plants using genomic tiling microarrays
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Cited by
Chromatin accessibility profiling by ATAC-seq
Grandi, F. C., Modi, H., Kampman, L., & Corces, M. R. (2022) · Nature Protocols · doi:10.1038/s41596-022-00692-9 · Cited by 557
RNA-sequencing from single nuclei
Grindberg, R. V., Yee-Greenbaum, J. L., McConnell, M. J., Novotny, M., O’Shaughnessy, A. L., Lambert, G. M., Araúzo-Bravo, M. J., Lee, J., Fishman, M., Robbins, G. E., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1319700110 · Cited by 422
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Self-citationCircadian Clock and Photoperiodic Flowering in Arabidopsis: CONSTANS Is a Hub for Signal Integration
Shim, J. S., Kubota, A., & Imaizumi, T. (2017) · Plant Physiology · doi:10.1104/pp.16.01327 · Cited by 359
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Metadata
- Authors
- Deal, R. B., & Henikoff, S.
- Venue
- Nature Protocols
- Published
- 2010-12-16
- Volume
- 6
- Issue
- 1
- Pages
- 56-68
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2094314820
- Cited by
- 386
- References
- 28
- FWCI
- 17.46
- Type
- journal article
- Language
- en
- Open access
- green
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