Cited by 437Microbial infections and disease researchViral Infectious Diseases and Gene Expression in InsectsBacterial Identification and Susceptibility Testing

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Detection of Mycoplasma in cell cultures

Young, Lesley, Sung, Julia, Stacey, Glyn, Masters, John R·2010·Nature Protocols

DOI 10.1038/nprot.2010.43

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Abstract

Mycoplasma is a prokaryotic organism that is a frequent and occult contaminant of cell cultures. This organism can modify many aspects of cell physiology, rendering experiments that are conducted with contaminated cells worthless. Because of their small size, Mycoplasmas can pass through filters used to prevent bacterial and fungal contamination and potentially spread to all the cultures in a laboratory. It is essential that all new cell cultures entering a laboratory and all cell banks are tested for the presence of Mycoplasma. It is recommended that two techniques be used, selected from a PCR-based method, indirect staining and an agar and broth culture. This protocol describes these three tests for detecting Mycoplasma, which take from 1 d to 3-4 weeks, and such tests should be an obligatory component of quality control in every tissue culture laboratory.

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Method
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Authors: Young, L., Sung, J., Stacey, G., & Masters, J. R.
Affiliations: University College London (GB)
Corresponding author: Masters, John R
ORCID: Stacey, Glyn
Venue: Nature Protocols
Publisher: Springer Science and Business Media LLC
Published: 2010-04-22
Volume: 5
Issue: 5
Pages: 929-934
ISSN: 1750-2799 (ISSN-L)
1754-2189
DOI: 10.1038/nprot.2010.43
OpenAlex: W2092105616
Cited by: 437
References: 11
Citations by year: 2026: 23 · 2025: 44 · 2024: 43 · 2023: 53 · 2022: 32 · 2021: 41 · 2020: 36 · 2019: 40 · 2018: 35 · 2017: 19 · 2016: 12 · 2015: 17 · 2014: 14 · 2013: 9 · 2012: 10
FWCI: 8.25
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature Protocols (publishedVersion)
PubMed (publishedVersion)
UCL Discovery (University College London) (submittedVersion)
Topics: Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
Keywords: mycoplasma detection, cell culture contamination, PCR detection, laboratory quality control, tissue culture
MeSH terms: Bacteriological Techniques, DNA, Bacterial, Electrophoresis, Agar Gel, Mycoplasma, Sensitivity and Specificity, Staining and Labeling, Time Factors, Polymerase Chain Reaction

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Detection of Mycoplasma in cell cultures

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