The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Isolation of adipose-derived stem cells and their induction to a chondrogenic phenotype
Open accessAbstract
The ability to isolate, expand and differentiate adult stem cells into a chondrogenic lineage is an important step in the development of tissue engineering approaches for cartilage repair or regeneration for the treatment of joint injury or osteoarthritis, as well as for their application in plastic or reconstructive surgery. Adipose-derived stem cells (ASCs) provide an abundant and easily accessible source of adult stem cells for use in such regenerative approaches. This protocol first describes the isolation of ASCs from liposuction aspirate. The cell culture conditions provided for ASC expansion provide a large number of multipotent stem cells. Instructions for growth factor–based induction of ASCs into chondrocyte-like cells using either cell pellet or alginate bead systems are detailed. These methods are similar to those published for chondrogenesis of bone marrow–derived mesenchymal stem cells but distinct because of the unique nature of ASCs. Investigators can expect consistent differentiation of ASCs, allowing for slight variation as a result of donor and serum lot effects. Approximately 10–12 weeks are needed for the entire process of ASC isolation, including the characterization of chondrocyte-like cells, which is also described.
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Cited by
Beane, O. S., Fonseca, V. C., Cooper, L. L., Koren, G., & Darling, E. M. (2014) · PLoS ONE · doi:10.1371/journal.pone.0115963 · Cited by 323
Wang, T., Lai, J. H., Han, L.-H., Tong, X., & Yang, F. (2014) · Tissue Engineering Part A · doi:10.1089/ten.tea.2013.0531 · Cited by 70
Bio-engineering a prevascularized human tri-layered skin substitute containing a hypodermis
Zimoch, J., Zielinska, D., Michalak-Micka, K., Rütsche, D., Böni, R., Biedermann, T., & Klar, A. S. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.07.033 · Cited by 57
Capella-Monsonís, H., De Pieri, A., Peixoto, R., Korntner, S., & Zeugolis, D. I. (2020) · Stem Cell Research & Therapy · doi:10.1186/s13287-020-02021-x · Cited by 42
Park, J., Jo, S., Lee, M.-K., Kim, T.-H., Sung, I.-H., & Lee, J. K. (2022) · Bone & Joint Research · doi:10.1302/2046-3758.1111.bjr-2021-0548.r2 · Cited by 12
Metadata
- Authors
- Estes, B. T., Diekman, B. O., Gimble, J. M., & Guilak, F.
- Venue
- Nature Protocols
- Published
- 2010-06-17
- Volume
- 5
- Issue
- 7
- Pages
- 1294-1311
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2018028722
- Cited by
- 428
- References
- 58
- FWCI
- 13.74
- Type
- journal article
- Language
- en
- Open access
- green
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