The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Liquid chromatography quadrupole time-of-flight mass spectrometry characterization of metabolites guided by the METLIN database
Open accessAbstract
Untargeted metabolomics provides a comprehensive platform for identifying metabolites whose levels are altered between two or more populations. By using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS), hundreds to thousands of peaks with a unique m/z ratio and retention time are routinely detected from most biological samples in an untargeted profiling experiment. Each peak, termed a metabolomic feature, can be characterized on the basis of its accurate mass, retention time and tandem mass spectral fragmentation pattern. Here a seven-step protocol is suggested for such a characterization by using the METLIN metabolite database. The protocol starts from untargeted metabolomic LC-Q-TOF-MS data that have been analyzed with the bioinformatics program XCMS, and it describes a strategy for selecting interesting features as well as performing subsequent targeted tandem MS. The seven steps described will require 2–4 h to complete per feature, depending on the compound.
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Cites
Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease
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Self-citationMass spectrometry‐based metabolomics
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METLIN—a metabolite mass spectral database
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Self-citationMetabolite profiling for plant functional genomics
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XCMS Online: A Web-Based Platform to Process Untargeted Metabolomic Data
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Cited by
Liu, X., & Locasale, J. W. (2017) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2017.01.004 · Cited by 453
NMR-based metabolomics in human disease diagnosis: applications, limitations, and recommendations
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SREBP-dependent lipidomic reprogramming as a broad-spectrum antiviral target
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A roadmap for the XCMS family of software solutions in metabolomics
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Metadata
- Authors
- Zhu, Z.-J., Schultz, A. W., Wang, J., Johnson, C. H., Yannone, S. M., Patti, G. J., & Siuzdak, G.
- Venue
- Nature Protocols
- Published
- 2013-02-07
- Volume
- 8
- Issue
- 3
- Pages
- 451-460
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2064988047
- Cited by
- 477
- References
- 47
- FWCI
- 16.27
- Type
- journal article
- Language
- en
- Open access
- green
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