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Mouse model of chronic and binge ethanol feeding (the NIAAA model)

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Bertola, Adeline, Mathews, Stephanie, Ki, Sung Hwan, Wang, Hua, Gao, Bin·2013·Nature Protocols
DOI 10.1038/nprot.2013.032
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Abstract

Chronic alcohol consumption is a leading cause of chronic liver disease worldwide, leading to cirrhosis and hepatocellular carcinoma. Currently, the most widely used model for alcoholic liver injury is ad libitum feeding with the Lieber-DeCarli liquid diet containing ethanol for 4–6 weeks; however, this model, without the addition of a secondary insult, only induces mild steatosis, slight elevation of serum alanine transaminase (ALT) and little or no inflammation. Here we describe a simple mouse model of alcoholic liver injury by chronic ethanol feeding (10-d ad libitum oral feeding with the Lieber-DeCarli ethanol liquid diet) plus a single binge ethanol feeding. This protocol for chronic-plus-single-binge ethanol feeding synergistically induces liver injury, inflammation and fatty liver, which mimics acute-on-chronic alcoholic liver injury in patients. This feeding protocol can also be extended to chronic feeding for longer periods of time up to 8 weeks plus single or multiple binges. Chronic-binge ethanol feeding leads to high blood alcohol levels; thus, this simple model will be very useful for the study of alcoholic liver disease (ALD) and of other organs damaged by alcohol consumption.

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Metadata

Authors
Bertola, A., Mathews, S., Ki, S. H., Wang, H., & Gao, B.
Venue
Nature Protocols
Publisher
Springer Science and Business Media LLC
Published
2013-02-28
Volume
8
Issue
3
Pages
627-637
ISSN
  • 1750-2799 (ISSN-L)
  • 1754-2189
DOI
10.1038/nprot.2013.032
OpenAlex
W2024933805
Cited by
1,213
References
5
FWCI
28.68
Type
journal article
Language
en
Open access
green
Licence
www.springer.com/tdm (tdm)

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CoChat. (2026). Citation check: Mouse model of chronic and binge ethanol feeding (the NIAAA model). https://app.cochat.ai/papers/10.1038/nprot.2013.032
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Bertola, A., Mathews, S., Ki, S. H., Wang, H., & Gao, B. (2013). Mouse model of chronic and binge ethanol feeding (the NIAAA model). Nature Protocols. https://doi.org/10.1038/nprot.2013.032

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