The regulatory activity of microRNA* species has substantial influence on microRNA and 3′ UTR evolution
Abstract
During microRNA (miRNA) biogenesis, one strand of a approximately 21-22-nucleotide RNA duplex is preferentially selected for entry into a silencing complex. The other strand, known as the miRNA* species, has typically been assumed to be a carrier strand. Here we show that, although Drosophila melanogaster miRNA* species are less abundant than their partners, they are often present at physiologically relevant levels and can associate with Argonaute proteins. Comparative genomic analyses revealed that >40% of miRNA* sequences resist nucleotide divergence across Drosophilid evolution, and at least half of these well-conserved miRNA* species select for conserved 3' untranslated region seed matches well above background noise. Finally, we validated the inhibitory activity of miRNA* species in both cultured cells and transgenic animals. These data broaden the reach of the miRNA regulatory network and suggest an important mechanism that diversifies miRNA function during evolution.
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Less abundant miR* strands were shown to repress mRNAs containing predicted miR* target sites in flies and mammals, suggesting that miR* strands are functional.
“Consistent with this idea, the less abundant miR* strands were shown repress mRNAs containing predicted miR* target sites in flies and mammals, suggesting that miR* strands are functional (Okamura et al., 2008; Yang et al., 2010).”
From microRNA strand selection: Unwinding the rules (2021) · cites this paper as Okamura et al., 2008
For the majority of miRNAs, both strands have precisely defined 5' termini compared to their 3' ends, suggesting evolutionary pressure to maintain the target-determining sequence of both strands.
“In addition, for majority of miRNAs, both strands have precisely defined 5′ termini, compared to their 3′ ends, suggesting evolutionary pressure to maintain the target‐determining sequence of both strands (Okamura et al., 2008; Ruby, Jan, et al., 2007; Ruby, Stark, et al., 2007; Seitz, Ghildiyal, & Zamore, 2008).”
From microRNA strand selection: Unwinding the rules (2021) · cites this paper as Okamura et al., 2008
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The Widespread Impact of Mammalian MicroRNAs on mRNA Repression and Evolution
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Stark, A., Brennecke, J., Bushati, N., Russell, R. B., & Cohen, S. M. (2005) · Cell · doi:10.1016/j.cell.2005.11.023 · Cited by 1,080 · see citing works
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Identifying Functional MicroRNAs in Macrophages with Polarized Phenotypes
Graff, J. W., Dickson, A. M., Clay, G., McCaffrey, A. P., & Wilson, M. E. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.327031 · Cited by 367 · see citing works
The regulation of mRNA stability in mammalian cells: 2.0
Wu, X., & Brewer, G. (2012) · Gene · doi:10.1016/j.gene.2012.03.021 · Cited by 239 · see citing works
microRNA strand selection: Unwinding the rules
Medley, J. C., Panzade, G., & Zinovyeva, A. Y. (2021) · WIREs RNA · doi:10.1002/wrna.1627 · Cited by 225 · see citing works
A Regulatory Role for MicroRNA 33* in Controlling Lipid Metabolism Gene Expression
Goedeke, L., Vales-Lara, F. M., Fenstermaker, M., Cirera-Salinas, D., Chamorro-Jorganes, A., Ramírez, C. M., Mattison, J. A., de Cabo, R., Suárez, Y., & Fernández-Hernando, C. (2013) · Molecular and Cellular Biology · doi:10.1128/mcb.01714-12 · Cited by 140 · see citing works
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- Authors
- Okamura, K., Phillips, M. D., Tyler, D. M., Duan, H., Chou, Y.-T., & Lai, E. C.
- Venue
- Nature Structural & Molecular Biology
- Published
- 2008-03-30
- Volume
- 15
- Issue
- 4
- Pages
- 354-363
- ISSN
- 1545-9993
- 1545-9985
- Cited by
- 461
- References
- 2
- Language
- en
- Licence
- www.springer.com/tdm (tdm)
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