Postabsorptive VLDL‐TG Fatty Acid Storage in Adipose Tissue in Lean and Obese Women
Open accessAbstract
Adipose tissue lipoprotein lipase (LPL) is a necessary enzyme for storage of very‐low‐density lipoprotein–triglyceride (VLDL‐TG), but whether it is a rate‐determining step is unknown. To test this hypothesis we included 10 upper‐body obese (UBO), 11 lower‐body obese (LBO), and 8 lean women. We infused ex vivo‐labeled VLDL‐14 C‐TG and then performed adipose tissue biopsies to understand the relationship between VLDL‐TG storage and LPL activity in femoral and upper‐body subcutaneous fat. Both fractional tracer storage and rate of storage of the VLDL‐TG tracer were evaluated. VLDL‐TG storage was also examined as a function of regional adipose tissue blood flow (ATBF), insulin, VLDL‐TG turnover, regional fat mass, fat‐free mass (FFM), and fat cell size. LPL activity per adipocyte was significantly greater in obese than lean women but not significantly different per gram lipid. Both VLDL‐TG fractional tracer storage per kg lipid and VLDL‐TG storage rate per kg lipid were similar in abdominal and femoral fat in all three groups and were not significantly different between groups. Multiple regression analysis identified FFM and femoral fat mass as significant independent predictors of VLDL‐TG fractional tracer storage and insulin as a significant predictor of VLDL‐TG fatty acid storage rate. LPL activity, ATBF, and VLDL‐TG turnover did not predict VLDL‐TG storage. We conclude that lower FFM and greater plasma insulin are associated with greater VLDL‐TG deposition in abdominal subcutaneous and femoral fat. Greater femoral fat mass signals greater femoral VLDL‐TG storage. We suggest that the differences in VLDL‐TG storage in abdominal and femoral fat that occur with progressive obesity are regulated through mechanisms other than LPL activity.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Some magnetic resonance spectroscopy studies found no difference in intramyocellular lipid concentrations between sexes.
“although some investigators have found similar intramyocellular lipid (IMCL) concentrations using magnetic resonance spectroscopy between sexes (80, 81)”
From Sex differences in the metabolism of glucose and fatty acids by adipose tissue and skeletal muscle in humans (2025) · cites this paper as [80]
The Methods and Procedures section describes a study of women only, and the VLDL-TG storage section compares abdominal and femoral adipose tissue, not intramyocellular lipid concentrations between sexes. Searches for intramyocellular lipid, magnetic resonance spectroscopy, and male–female muscle-lipid comparisons found no passage addressing the claim. (LLM, conf=0.78)
Experimental — accuracy not yet measuredStudies on muscle imTG usage during fasting exercise report conflicting results: some found imTG use only in women, others greater use in men, and others similar use between sexes.
“Regarding the quantification of muscle imTG usage during fasting state exercise, some authors reported imTG use only in women but not men (78, 79, 176), while others proposed the greater use in men (82) or found similar imTG use between sexes (80, 81).”
From Sex differences in the metabolism of glucose and fatty acids by adipose tissue and skeletal muscle in humans (2025) · cites this paper as [80]
The Methods and Procedures and VLDL-TG storage sections describe postabsorptive fatty-acid storage in adipose tissue in women, not muscle imTG use during exercise. Searches for imTG, exercise, and sex differences found no passage addressing the claimed conflicting results. (LLM, conf=0.76)
Experimental — accuracy not yet measuredGreater imTG contact with mitochondria has been reported in women after fasting exercise.
“It has been reported that there is greater imTG contact with mitochondria in women after exercise during fasting (80).”
From Sex differences in the metabolism of glucose and fatty acids by adipose tissue and skeletal muscle in humans (2025) · cites this paper as [80]
The Experimental protocol (page not available) says participants avoided heavy exercise before the study and remained in bed on the examination day. The paper examines VLDL-TG storage in adipose tissue, not intramuscular triglyceride contact with mitochondria. Searches for fasting exercise and mitochondrial contact found no passage addressing the reported finding. (LLM, conf=0.77)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 32 · Cited by 25
Research works printed but not linked: 1
- 1930739x, 2010, 7, Downloaded from https://onlinelibrary.wiley.com/doi/10.1038/oby.2009.370 by Test, Wiley Online Library on [20/08/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License · DOI
- Authors
- Nellemann, B., Gormsen, L. C., Christiansen, J. S., Jensen, M. D., & Nielsen, S.
- Affiliations
- Aarhus University Hospital (DK)
- Mayo Clinic (US)
- Corresponding author
- Nielsen, Søren
- Venue
- Obesity
- Publisher
- Wiley
- Published
- 2010-07
- Volume
- 18
- Issue
- 7
- Pages
- 1304-1311
- ISSN
- 1930-7381 (ISSN-L)
- 1930-739X
- PMC
- PMC2893248
- OpenAlex
- W2081742869
- Cited by
- 25
- References
- 32
- Citations by year
- 2025: 2 · 2024: 1 · 2021: 1 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 1 · 2012: 6
- FWCI
- 2.37
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Obesity (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- American Diabetes Association
- Sundhed og Sygdom, Det Frie Forskningsråd
- Novo Nordisk Fonden
- National Institutes of Health
- Medical Research Council
- National Institute of Diabetes and Digestive and Kidney Diseases (DK 45343, R01DK045343)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- adipose tissue lipoprotein lipase, subcutaneous adipose tissue, abdominal fat, fat-free mass, insulin, adipose tissue blood flow, adipocyte size, women
- MeSH terms
- Adipose Tissue, Adult, Biopsy, Body Composition, Body Weight, Female, Humans, Insulin, Lipoprotein Lipase, Lipoproteins, VLDL, Middle Aged, Obesity, Regression Analysis, Triglycerides, Multivariate Analysis, Cell Size, Body Fat Distribution
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
