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Cited by 79Cancer-related molecular mechanisms researchExtracellular vesicles in diseaseThyroid Cancer Diagnosis and Treatment

Exosomal lncRNA DOCK9-AS2 derived from cancer stem cell-like cells activated Wnt/β-catenin pathway to aggravate stemness, proliferation, migration, and invasion in papillary thyroid carcinoma

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Dai, Wencheng, Jin, Xiaoxia, Han, Liang, Huang, Haijing, Ji, Zhenhua, Xu, Xinjiang, Tang, Mingming, Jiang, Bin +1 more·2020·Cell Death & Disease
DOI 10.1038/s41419-020-02827-wPMC PMC7486896
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Abstract

Exosomal long non-coding RNAs (lncRNAs) are crucial factors that mediate the extracellular communication in tumor microenvironment. DOCK9 antisense RNA2 (DOCK9-AS2) is an exosomal lncRNA which has not been investigated in papillary thyroid carcinoma (PTC). Based on the result of differentially expressed lncRNAs in PTC via bioinformatics databases, we discovered that DOCK9-AS2 was upregulated in PTC, and presented elevation in plasma exosomes of PTC patients. Functionally, DOCK9-AS2 knockdown reduced proliferation, migration, invasion, epithelial-to-mesenchymal (EMT) and stemness in PTC cells. PTC-CSCs transmitted exosomal DOCK9-AS2 to improve stemness of PTC cells. Mechanistically, DOCK9-AS2 interacted with SP1 to induce catenin beta 1 (CTNNB1) transcription and sponged microRNA-1972 (miR-1972) to upregulate CTNNB1, thereby activating Wnt/β-catenin pathway in PTC cells. In conclusion, PTC-CSCs-derived exosomal lncRNA DOCK9-AS2 activated Wnt/β-catenin pathway to aggravate PTC progression, indicating that DOCK9-AS2 was a potential target for therapies in PTC.

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  • DOCK9 knockdown leads to reduced proliferation and migratory characteristics in papillary thyroid carcinoma.

    “DOCK9 was recently implicated as a target for the alleviation of papillary thyroid carcinoma due to knockdown leading to reduced proliferation and migratory characteristics (123).”

    From RHO to the DOCK for GDP disembarking: Structural insights into the DOCK GTPase nucleotide exchange factors (2021) · cites this paper as [123]

    The Results section, “DOCK9-AS2 silence abrogated proliferation, migration, invasion, EMT, and stemness of PTC cells” (page unavailable), reports reduced proliferation and migration after DOCK9-AS2 knockdown. The cited paper studies knockdown of the lncRNA DOCK9-AS2, not DOCK9 itself, so it does not support the target named in the claim. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Dai, W., Jin, X., Han, L., Huang, H., Ji, Z., Xu, X., Tang, M., Jiang, B., & Chen, W.
Affiliations
Nantong University (CN)
Corresponding author
Jiang, Bin
ORCID
Venue
Cell Death & Disease
Publisher
Springer Science and Business Media LLC
Published
2020-09-11
Volume
11
Issue
9
Pages
743-743
ISSN
2041-4889 (ISSN-L)
DOI
10.1038/s41419-020-02827-w
PMC
PMC7486896
OpenAlex
W3086820975
Cited by
79
References
1
Citations by year
2026: 8 · 2025: 20 · 2024: 10 · 2023: 11 · 2022: 17 · 2021: 11 · 2020: 2
FWCI
3.51
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell Death and Disease (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
The study was supported by the Science and Technology Project of NanTong
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Cancer Diagnosis and Treatment
Keywords
long non-coding RNA, exosomes, papillary thyroid carcinoma, cancer stem cells, Wnt/β-catenin signaling, CTNNB1, Sp1, epithelial-mesenchymal transition, cancer stemness, cell proliferation, cell migration and invasion, plasma exosomes, tumor microenvironment
MeSH terms
Thyroid Cancer, Papillary, Animals, Cell Movement, Humans, Male, Mice, Nude, Neoplasm Invasiveness, Transfection, Neoplastic Stem Cells, Guanine Nucleotide Exchange Factors, Cell Proliferation, beta Catenin, Mice, Exosomes, RNA, Long Noncoding

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