Cited by 102Metal-Catalyzed Oxygenation MechanismsEnzyme Structure and FunctionGeochemistry and Elemental Analysis

Biogenic manganese oxide nanoparticle formation by a multimeric multicopper oxidase Mnx

Open access
Romano, Christine A., Zhou, Mowei, Song, Yang, Wysocki, Vicki H., Dohnalkova, Alice C., Kovarik, Libor, Paša-Tolić, Ljiljana, Tebo, Bradley M.·2017·Nature Communications

Abstract

Bacteria that produce Mn oxides are extraordinarily skilled engineers of nanomaterials that contribute significantly to global biogeochemical cycles. Their enzyme-based reaction mechanisms may be genetically tailored for environmental remediation applications or bioenergy production. However, significant challenges exist for structural characterization of the enzymes responsible for biomineralization. The active Mn oxidase in Bacillus sp. PL-12, Mnx, is a complex composed of a multicopper oxidase (MCO), MnxG, and two accessory proteins, MnxE and MnxF. MnxG shares sequence similarity with other, structurally characterized MCOs. MnxE and MnxF have no similarity to any characterized proteins. The ~200 kDa complex has been recalcitrant to crystallization, so its structure is unknown. Here, we show that native mass spectrometry defines the subunit topology and copper binding of Mnx, while high-resolution electron microscopy visualizes the protein and nascent Mn oxide minerals. These data provide critical structural information for understanding Mn biomineralization by such unexplored enzymes.

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  • Heterocomplexes that have multiple subunits with very similar masses are difficult to characterise just from the intact mass, especially when there is high uncertainty in mass measurement.

    “Still, heterocomplexes that have multiple subunits with very similar masses are difficult to characterise just from the intact mass, especially when there is high uncertainty in mass measurement.”

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  1. Zhou M., et al. Unveiling the heterogeneity of an uncharacterized manganese oxidizing multicopper oxidase using high resolution native mass spectrometry and surface induced dissociation. Proc. 64th Am. Soc. Mass Spectrom. Conf. 279055 (2016).

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Authors
Romano, C. A., Zhou, M., Song, Y., Wysocki, V. H., Dohnalkova, A. C., Kovarik, L., Paša-Tolić, L., & Tebo, B. M.
Affiliations
  • Oregon Health & Science University (US)
  • Pacific Northwest National Laboratory (US)
  • The Ohio State University (US)
Corresponding author
  • Romano, Christine A.
  • Zhou, Mowei
  • Tebo, Bradley M.
Venue
Nature Communications
Published
2017-09-29
Volume
8
Issue
1
Pages
746-746
ISSN
2041-1723 (ISSN-L)
OpenAlex
W2760615945
Cited by
102
References
66
Citations by year
2026: 11 · 2025: 14 · 2024: 10 · 2023: 12 · 2022: 11 · 2021: 14 · 2020: 11 · 2019: 13 · 2018: 6
FWCI
3.14
Type
journal article
Language
en
Open access
Locations
  • Nature Communications (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Science Foundation (1410688, 1202859)
  • U.S. Department of Energy
  • Bentham-Moxon Trust
  • National Institutes of Health
  • Office of Science
  • National Institute of General Medical Sciences (R01GM113658)
  • Division of Chemistry (CHE-1410688)
  • Biological and Environmental Research
  • Pacific Northwest National Laboratory
Topics
  • Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
  • Physical Sciences › Earth and Planetary Sciences › Geochemistry and Petrology › Geochemistry and Elemental Analysis
Keywords
biogenic manganese oxides, multicopper oxidase, manganese nanoparticles, copper binding, native mass spectrometry, high-resolution electron microscopy
MeSH terms
Bacillus, Bacterial Proteins, Copper, Manganese, Oxides, Oxidoreductases, Mass Spectrometry, Manganese Compounds, Microscopy, Electron, Transmission, Nanoparticles

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