The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
Open accessAbstract
The emergence and spread of Zika virus (ZIKV) presented a challenge to the diagnosis of ZIKV infections in areas with transmission of dengue (DENV) and chikungunya (CHIKV) viruses. To facilitate detection of ZIKV infections, and differentiate these infections from DENV and CHIKV, we developed the Trioplex real-time RT-PCR assay (Trioplex assay). Here, we describe the optimization of multiplex and singleplex formats of the assay for a variety of chemistries and instruments to facilitate global standardization and implementation. We evaluated the analytical performance of all Trioplex modalities for detection of these three pathogens in serum and whole blood, and for ZIKV in urine. The limit of detection for the three viruses and in different RNA-extraction modalities is near 10 3 genome copy equivalents per milliliter (GCE/mL). Simultaneous testing of more than one specimen type from each patient provides a 6.4% additional diagnostic sensitivity. Overall, the high sensitivity of the Trioplex assay demonstrates the utility of this assay ascertaining Zika cases.
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Cites
Zika Virus Outbreak on Yap Island, Federated States of Micronesia
Duffy, M. R., Chen, T.-H., Hancock, W. T., Powers, A. M., Kool, J. L., Lanciotti, R. S., Pretrick, M., Marfel, M., Holzbauer, S., Dubray, C., et al. (2009) · New England Journal of Medicine · doi:10.1056/nejmoa0805715 · Cited by 3,132
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Lanciotti, R. S., Kosoy, O. L., Laven, J. J., Velez, J. O., Lambert, A. J., Johnson, A. J., Stanfield, S. M., & Duffy, M. R. (2008) · Emerging Infectious Diseases · doi:10.3201/eid1408.080287 · Cited by 2,173
Zika Virus and Birth Defects — Reviewing the Evidence for Causality
Rasmussen, S. A., Jamieson, D. J., Honein, M. A., & Petersen, L. R. (2016) · New England Journal of Medicine · doi:10.1056/nejmsr1604338 · Cited by 1,989
Potential Sexual Transmission of Zika Virus
Musso, D., Roche, C., Robin, E., Nhan, T., Teissier, A., & Cao-Lormeau, V.-M. (2015) · Emerging Infectious Diseases · doi:10.3201/eid2102.141363 · Cited by 1,176
Dejnirattisai, W., Supasa, P., Wongwiwat, W., Rouvinski, A., Barba-Spaeth, G., Duangchinda, T., Sakuntabhai, A., Cao-Lormeau, V.-M., Malasit, P., Rey, F. A., et al. (2016) · Nature Immunology · doi:10.1038/ni.3515 · Cited by 914
Evidence of Sexual Transmission of Zika Virus
D’Ortenzio, E., Matheron, S., de Lamballerie, X., Hubert, B., Piorkowski, G., Maquart, M., Descamps, D., Damond, F., Yazdanpanah, Y., & Leparc-Goffart, I. (2016) · New England Journal of Medicine · doi:10.1056/nejmc1604449 · Cited by 716
Detection of Zika virus in saliva
Musso, D., Roche, C., Nhan, T.-X., Robin, E., Teissier, A., & Cao-Lormeau, V.-M. (2015) · Journal of Clinical Virology · doi:10.1016/j.jcv.2015.04.021 · Cited by 521
Chikungunya Virus in US Travelers Returning from India, 2006
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Cited by
Point-of-care diagnostics for infectious diseases: From methods to devices
Wang, C., Liu, M., Wang, Z., Li, S., Deng, Y., & He, N. (2021) · Nano Today · doi:10.1016/j.nantod.2021.101092 · Cited by 622
Thézé, J., Li, T., du Plessis, L., Bouquet, J., Kraemer, M. U. G., Somasekar, S., Yu, G., de Cesare, M., Balmaseda, A., Kuan, G., et al. (2018) · Cell Host & Microbe · doi:10.1016/j.chom.2018.04.017 · Cited by 106
Metadata
- Authors
- Santiago, G. A., Vázquez, J., Courtney, S., Matías, K. Y., Andersen, L. E., Colón, C., Butler, A. E., Roulo, R., Bowzard, J., Villanueva, J. M., & Muñoz-Jordan, J. L.
- Venue
- Nature Communications
- Published
- 2018-04-11
- Volume
- 9
- Issue
- 1
- Pages
- 1391-1391
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2796800715
- Cited by
- 264
- References
- 31
- FWCI
- 29.73
- Type
- journal article
- Language
- en
- Open access
- gold
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- Santiago, G. A., Vázquez, J., Courtney, S., Matías, K. Y., Andersen, L. E., Colón, C., Butler, A. E., Roulo, R., Bowzard, J., Villanueva, J. M., & Muñoz-Jordan, J. L. (2018). Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses. Nature Communications. https://doi.org/10.1038/s41467-018-03772-1
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