The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Serotype-Specific Detection of Dengue Viruses in a Fourplex Real-Time Reverse Transcriptase PCR Assay
Open accessAbstract
The dengue (DEN) viruses are positive-strand RNA viruses in the genus Flavivirus . Dengue fever and dengue hemorrhagic fever/dengue shock syndrome are important human arboviral diseases caused by infection with one of four closely related but serologically distinct DEN viruses, designated DEN-1, DEN-2, DEN-3, and DEN-4 viruses. All four DEN serotypes are currently cocirculating throughout the subtropics and tropics, and genotypic variation occurs among isolates within a serotype. A real-time quantitative nucleic acid amplification assay has been developed to detect viral RNA of a single DEN virus serotype. Each primer-probe set is DEN serotype specific, yet detects all genotypes in a panel of 7 to 10 representative isolates of a serotype. In single reactions and in fourplex reactions (containing four primer-probe sets in a single reaction mixture), standard dilutions of virus equivalent to 0.002 PFU of DEN-2, DEN-3, and DEN-4 viruses were detected; the limit of detection of DEN-1 virus was 0.5 equivalent PFU. Singleplex and fourplex reactions were evaluated in a panel of 40 viremic serum specimens with 10 specimens per serotype, containing 0.002 to 6,000 equivalent PFU/reaction (0.4 to 1.2 × 10 6 PFU/ml). Viral RNA was detected in all viremic serum specimens in singleplex and fourplex reactions. Thus, this serotype-specific, fourplex real-time reverse transcriptase PCR nucleic acid detection assay can be used as a method for differential diagnosis of a specific DEN serotype in viremic dengue patients and as a tool for rapid identification and serotyping of DEN virus isolates.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Dengue and Dengue Hemorrhagic Fever
Gubler, D. J. (1998) · Clinical Microbiology Reviews · doi:10.1128/cmr.11.3.480 · Cited by 4,310
Lanciotti, R. S., Calisher, C. H., Gubler, D. J., Chang, G. J., & Vorndam, A. V. (1992) · Journal of Clinical Microbiology · doi:10.1128/jcm.30.3.545-551.1992 · Cited by 1,818
Self-citationThe Arboviruses: Epidemiology and Ecology
Monath, T. P. (2019) · CRC Press eBooks · doi:10.1201/9780429289170 · Cited by 1,240
Lanciotti, R. S., Kerst, A. J., Nasci, R. S., Godsey, M. S., Mitchell, C. J., Savage, H. M., Komar, N., Panella, N. A., Allen, B. C., Volpe, K. E., et al. (2000) · Journal of Clinical Microbiology · doi:10.1128/jcm.38.11.4066-4071.2000 · Cited by 1,087
Self-citationDengue haemorrhagic fever: diagnosis, treatment, prevention and control
Crane, G. (1999) · Pathology · doi:10.1016/s0031-3025(16)34854-1 · Cited by 744
Molecular evolution and distribution of dengue viruses type 1 and 2 in nature
Rico-Hesse, R. (1990) · Virology · doi:10.1016/0042-6822(90)90102-w · Cited by 593
Martin, D. A., Muth, D. A., Brown, T., Johnson, A. J., Karabatsos, N., & Roehrig, J. T. (2000) · Journal of Clinical Microbiology · doi:10.1128/jcm.38.5.1823-1826.2000 · Cited by 477
Molecular evolution and epidemiology of dengue-3 viruses
Lanciotti, R. S., Lewis, J. G., Gubler, D. J., & Trent, D. W. (1994) · Journal of General Virology · doi:10.1099/0022-1317-75-1-65 · Cited by 356
Self-citationPhylogenetic Relationships and Differential Selection Pressures among Genotypes of Dengue-2 Virus
Twiddy, S. S., Farrar, J. J., Vinh Chau, N., Wills, B., Gould, E. A., Gritsun, T., Lloyd, G., & Holmes, E. C. (2002) · Virology · doi:10.1006/viro.2002.1447 · Cited by 273
Lanciotti, R. S., & Kerst, A. J. (2001) · Journal of Clinical Microbiology · doi:10.1128/jcm.39.12.4506-4513.2001 · Cited by 267
Self-citation
Cited by
Dengue: a continuing global threat
Guzman, M. G., Halstead, S. B., Artsob, H., Buchy, P., Farrar, J., Gubler, D. J., Hunsperger, E., Kroeger, A., Margolis, H. S., Martínez, E., et al. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2460 · Cited by 2,082
Zika Virus Disrupts Neural Progenitor Development and Leads to Microcephaly in Mice
Li, C., Xu, D., Ye, Q., Hong, S., Jiang, Y., Liu, X., Zhang, N., Shi, L., Qin, C.-F., & Xu, Z. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.04.017 · Cited by 749
Detection of Zika virus in saliva
Musso, D., Roche, C., Nhan, T.-X., Robin, E., Teissier, A., & Cao-Lormeau, V.-M. (2015) · Journal of Clinical Virology · doi:10.1016/j.jcv.2015.04.021 · Cited by 521
Santiago, G. A., Vázquez, J., Courtney, S., Matías, K. Y., Andersen, L. E., Colón, C., Butler, A. E., Roulo, R., Bowzard, J., Villanueva, J. M., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-03772-1 · Cited by 264
History, epidemiology and diagnostics of dengue in the American and Brazilian contexts: a review
Salles, T. S., da Encarnação Sá-Guimarães, T., de Alvarenga, E. S. L., Guimarães-Ribeiro, V., de Meneses, M. D. F., de Castro-Salles, P. F., dos Santos, C. R., do Amaral Melo, A. C., Soares, M. R., Ferreira, D. F., et al. (2018) · Parasites & Vectors · doi:10.1186/s13071-018-2830-8 · Cited by 256
Metadata
- Authors
- Johnson, B. W., Russell, B. J., & Lanciotti, R. S.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2005-10
- Volume
- 43
- Issue
- 10
- Pages
- 4977-4983
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2118441980
- Cited by
- 494
- References
- 22
- FWCI
- 7.44
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Serotype-Specific Detection of Dengue Viruses in a Fourplex Real-Time Reverse Transcriptase PCR Assay. https://app.cochat.ai/papers/10.1128/jcm.43.10.4977-4983.2005
- The paper
- Johnson, B. W., Russell, B. J., & Lanciotti, R. S. (2005). Serotype-Specific Detection of Dengue Viruses in a Fourplex Real-Time Reverse Transcriptase PCR Assay. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.43.10.4977-4983.2005
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
