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Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole

Liu, Dazhi, Wei, Yifeng, Liu, Xuyang, Zhou, Yan, Jiang, Li, Yin, Jinyu, Wang, Feifei, Hu, Yiling, Nanjaraj Urs, Ankanahalli N., Liu, Yanhong, Ang, Ee Lui, Zhao, Suwen, Zhao, Huimin, Zhang, Yan·2018·Nature Communications
DOI 10.1038/s41467-018-06627-xPMC PMC6181972
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Abstract

Skatole is a malodorous compound that contributes to the characteristic smell of animal faeces. Although skatole has long been known to originate from bacterial tryptophan fermentation, the enzyme catalysing its formation has so far remained elusive. Here we report the use of comparative genomics for the discovery of indoleacetate decarboxylase, an O 2-sensitive glycyl radical enzyme catalysing the decarboxylation of indoleacetate to form skatole as the terminal step of tryptophan fermentation in certain anaerobic bacteria. We describe its biochemical characterization and compare it to other glycyl radical decarboxylases. Indoleacetate decarboxylase may serve as a genetic marker for the identification of skatole-producing environmental and human-associated bacteria, with impacts on human health and the livestock industry.

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Metadata

Authors
Liu, D., Wei, Y., Liu, X., Zhou, Y., Jiang, L., Yin, J., Wang, F., Hu, Y., Nanjaraj Urs, A. N., Liu, Y., Ang, E. L., Zhao, S., Zhao, H., & Zhang, Y.
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2018-10-11
Volume
9
Issue
1
ISSN
2041-1723
DOI
10.1038/s41467-018-06627-x
PMC
PMC6181972
Cited by
63
References
3
Language
en
Licence
Funders
National Natural Science Foundation of China (31570060)

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