High Density DNA Data Storage Library via Dehydration with Digital Microfluidic Retrieval
Open accessAbstract
DNA promises to be a high density data storage medium, but physical storage poses a challenge. To store large amounts of data, pools must be physically isolated so they can share the same addressing scheme. We propose the storage of dehydrated DNA spots on glass as an approach for scalable DNA data storage. The dried spots can then be retrieved by a water droplet using a digital microfluidic device. Here we show that this storage schema works with varying spot organization, spotted masses of DNA, and droplet retrieval dwell times. In all cases, the majority of the DNA was retrieved and successfully sequenced. We demonstrate that the spots can be densely arranged on a microfluidic device without significant contamination of the retrieval. We also demonstrate that 1 TB of data could be stored in a single spot of DNA and successfully retrieved using this method.
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Newman et al. (2019) developed a method for preservation of dehydrated DNA spots on glass cartridges, recoverable by a water droplet; multiple DNA spots on one cartridge increase storage density to 50 TB of data per glass cartridge.
“Furthermore, Newman et al. (2019) developed a method for the preservation of dehydrated DNA spots on glass cartridges, which can subsequently be recovered by a water droplet. Multiple DNA spots on one cartridge additionally increase the storage density of 50 TB of data per glass cartridge [39].”
From DNA Data Storage. (2023) · cites this paper as [39]
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Experimental — accuracy not yet measuredDigital microfluidic was applied to automatically retrieve data from a high-density DNA data storage library.
“Newman et al. (2019) applied digital microfluidic to automatically retrieve data from high-density DNA data storage library.”
From Large-Scale de Novo Oligonucleotide Synthesis for Whole-Genome Synthesis and Data Storage: Challenges and Opportunities (2021) · cites this paper as Newman et al., 2019
DNA has become a promising tool for next-generation data storage since it provides high data capacity and storage density and can be stored in multiple ways over significant time periods.
“DNA has become a promising tool for next-generation data storage since it provides high data capacity and storage density78 and it is possible to store it in multiple ways37 over significant time periods.4”
From Emerging Approaches to DNA Data Storage: Challenges and Prospects (2022) · cites this paper as [37]
Because physical encapsulation separates file sequences from external barcodes, the file system offers long-term environmental protection of encoded file sequences via silica encapsulation for permanent archival storage, where external barcodes may be renewed periodically, further protected with secondary encapsulation, or data pools may be stored using methods implemented in PCR-based random
“Because physical encapsulation separates file sequences from external barcodes that are used to describe the encapsulated information, our file system offers long-term environmental protection of encoded file sequences via silica encapsulation for permanent archival storage9,23,24, where external barcodes may be renewed periodically, further protected with secondary encapsulation, or data pools”
From Random access DNA memory using Boolean search in an archival file storage system (2021) · cites this paper as [21]
Similar to PCR-based approaches, barcodes can be protected by dehydrating the data pool or immersing files in hydrophobic liquids.
“As also noted there, similar to PCR-based approaches, barcodes can be protected by dehydrating the data pool or immersing files in hydrophobic liquids21.”
From Random access DNA memory using Boolean search in an archival file storage system (2021) · cites this paper as [21]
The file system overcomes several challenges associated with pre-existing PCR-based file systems, including obviating the need for numerous heating and cooling cycles and enzymatic synthesis, and eliminating non-specific crosstalk between file sequences and barcodes, while enabling arbitrary Boolean logical search queries.
“Our file system overcomes several challenges associated with pre-existing PCR-based file systems, including obviating the need for numerous heating and cooling cycles and enzymatic synthesis, and eliminating non-specific crosstalk between file sequences and barcodes17,19,21, while enabling arbitrary Boolean logical search queries.”
From Random access DNA memory using Boolean search in an archival file storage system (2021) · cites this paper as [21]
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References 19 · Cited by 144
- Authors
- Newman, S., Stephenson, A. P., Willsey, M., Nguyen, B. H., Takahashi, C. N., Strauss, K., & Ceze, L.
- Affiliations
- University of Washington (US)
- Microsoft (United States) (US)
- ORCID
- Venue
- Nature Communications
- Published
- 2019-04-12
- Volume
- 10
- Issue
- 1
- Pages
- 1706-1706
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC6461645
- OpenAlex
- W2937055931
- Cited by
- 144
- References
- 19
- Citations by year
- 2026: 13 · 2025: 21 · 2024: 19 · 2023: 25 · 2022: 19 · 2021: 23 · 2020: 16 · 2019: 8
- FWCI
- 5.46
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- Funders
- Defense Advanced Research Projects Agency
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Biological Computing
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrowetting and Microfluidic Technologies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- DNA data storage, dehydration, glass substrates, digital microfluidics, high-density storage, DNA sequencing, data retrieval, storage scalability
- MeSH terms
- Desiccation, DNA, Equipment Design, Glass, Water, Information Storage and Retrieval, Computational Biology, Microfluidics, Lab-On-A-Chip Devices
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