The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules
Open accessAbstract
Besides genome editing, CRISPR-Cas12a has recently been used for DNA detection applications with attomolar sensitivity but, to our knowledge, it has not been used for the detection of small molecules. Bacterial allosteric transcription factors (aTFs) have evolved to sense and respond sensitively to a variety of small molecules to benefit bacterial survival. By combining the single-stranded DNA cleavage ability of CRISPR-Cas12a and the competitive binding activities of aTFs for small molecules and double-stranded DNA, here we develop a simple, supersensitive, fast and high-throughput platform for the detection of small molecules, designated CaT-SMelor (C RISPR-Cas12a- and aT F-mediated s mall m ol e cu l e detect or). CaT-SMelor is successfully evaluated by detecting nanomolar levels of various small molecules, including uric acid and p-hydroxybenzoic acid among their structurally similar analogues. We also demonstrate that our CaT-SMelor directly measured the uric acid concentration in clinical human blood samples, indicating a great potential of CaT-SMelor in the detection of small molecules.
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Cites
Gout-associated uric acid crystals activate the NALP3 inflammasome
Martinon, F., Pétrilli, V., Mayor, A., Tardivel, A., & Tschopp, J. (2006) · Nature · doi:10.1038/nature04516 · Cited by 5,326
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Chen, J. S., Ma, E., Harrington, L. B., Da Costa, M., Tian, X., Palefsky, J. M., & Doudna, J. A. (2018) · Science · doi:10.1126/science.aar6245 · Cited by 4,507
Anderson, N. L., & Anderson, N. G. (2002) · Molecular & Cellular Proteomics · doi:10.1074/mcp.r200007-mcp200 · Cited by 4,414
Safety assessment of esters of p-hydroxybenzoic acid (parabens)
Soni, M. G., Carabin, I. G., & Burdock, G. A. (2005) · Food and Chemical Toxicology · doi:10.1016/j.fct.2005.01.020 · Cited by 1,012
Cited by
Kaminski, M. M., Abudayyeh, O. O., Gootenberg, J. S., Zhang, F., & Collins, J. J. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00760-7 · Cited by 1,259
Xiong, Y., Zhang, J., Yang, Z., Mou, Q., Ma, Y., Xiong, Y., & Lu, Y. (2020) · Journal of the American Chemical Society · doi:10.1021/jacs.9b09211 · Cited by 689
Self-citationNucleic Acid Detection Using CRISPR/Cas Biosensing Technologies
Aman, R., Mahas, A., & Mahfouz, M. (2020) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00507 · Cited by 409
CRISPR-Cas12a-driven MXene-PEDOT:PSS piezoresistive wireless biosensor
Zeng, R., Wang, W., Chen, M., Wan, Q., Wang, C., Knopp, D., & Tang, D. (2021) · Nano Energy · doi:10.1016/j.nanoen.2020.105711 · Cited by 394
Self-citationFeng, W., Newbigging, A. M., Tao, J., Cao, Y., Peng, H., Le, C., Wu, J., Pang, B., Li, J., Tyrrell, D. L., et al. (2021) · Chemical Science · doi:10.1039/d0sc06973f · Cited by 312
Metadata
- Authors
- Liang, M., Li, Z., Wang, W., Liu, J., Liu, L., Zhu, G., Karthik, L., Wang, M., Wang, K.-F., Wang, Z., Yu, J., Shuai, Y., Yu, J., Zhang, L., Yang, Z., Li, C., Zhang, Q., Shi, T., Zhou, L., Xie, F., Dai, H., Liu, X., Zhang, J., Liu, G., Zhuo, Y., Zhang, B., Liu, C., Li, S., Xia, X., Tong, Y., Liu, Y., Alterovitz, G., Tan, G.-Y., & Zhang, L.-X.
- Venue
- Nature Communications
- Published
- 2019-08-14
- Volume
- 10
- Issue
- 1
- Pages
- 3672-3672
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2967373993
- Cited by
- 430
- References
- 4
- FWCI
- 13.89
- Type
- journal article
- Language
- en
- Open access
- gold
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- Liang, M., Li, Z., Wang, W., Liu, J., Liu, L., Zhu, G., Karthik, L., Wang, M., Wang, K.-F., Wang, Z., Yu, J., Shuai, Y., Yu, J., Zhang, L., Yang, Z., Li, C., Zhang, Q., Shi, T., Zhou, L., . . . Zhang, L.-X. (2019). A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules. Nature Communications. https://doi.org/10.1038/s41467-019-11648-1
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
