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Cited by 157Pancreatic function and diabetesDiabetes and associated disordersTissue Engineering and Regenerative Medicine

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Insulin-producing organoids engineered from islet and amniotic epithelial cells to treat diabetes

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Lebreton, Fanny, Lavallard, Vanessa, Bellofatto, Kevin, Bonnet, Romain, Wassmer, Charles H., Perez, Lisa, Kalandadze, Vakhtang, Follenzi, Antonia, Boulvain, Michel, Kerr-Conte, Julie, Goodman, David J., Bosco, Domenico, Berney, Thierry, Berishvili, Ekaterine·2019·Nature Communications
DOI 10.1038/s41467-019-12472-3PMC PMC6776618
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Abstract

Maintaining long-term euglycemia after intraportal islet transplantation is hampered by the considerable islet loss in the peri-transplant period attributed to inflammation, ischemia and poor angiogenesis. Here, we show that viable and functional islet organoids can be successfully generated from dissociated islet cells (ICs) and human amniotic epithelial cells (hAECs). Incorporation of hAECs into islet organoids markedly enhances engraftment, viability and graft function in a mouse type 1 diabetes model. Our results demonstrate that the integration of hAECs into islet cell organoids has great potential in the development of cell-based therapies for type 1 diabetes. Engineering of functional mini-organs using this strategy will allow the exploration of more favorable implantation sites, and can be expanded to unlimited (stem-cell-derived or xenogeneic) sources of insulin-producing cells.

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Metadata

Authors
Lebreton, F., Lavallard, V., Bellofatto, K., Bonnet, R., Wassmer, C. H., Perez, L., Kalandadze, V., Follenzi, A., Boulvain, M., Kerr-Conte, J., Goodman, D. J., Bosco, D., Berney, T., & Berishvili, E.
Affiliations
  • University of Geneva (CH)
  • Università degli Studi del Piemonte Orientale “Amedeo Avogadro” (IT)
  • Inserm (FR)
  • St Vincent's Hospital Melbourne (AU)
Corresponding author
Berishvili, Ekaterine
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2019-10-03
Volume
10
Issue
1
Pages
4491-4491
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-019-12472-3
PMC
PMC6776618
OpenAlex
W2979290450
Cited by
157
References
49
Citations by year
2026: 10 · 2025: 22 · 2024: 19 · 2023: 17 · 2022: 39 · 2021: 21 · 2020: 29
FWCI
21.24
Type
journal article
Language
en
Update notices
correction (2020-10-09)
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Archive ouverte UNIGE (University of Geneva) (publishedVersion, other-oa)
  • Minerva Access (University of Melbourne) (submittedVersion, cc-by)
  • HAL (submittedVersion, cc-by)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • LillOA (Université de Lille (University Of Lille)) (submittedVersion)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
  • VUBIR (Vrije Universiteit Brussel) (submittedVersion)
Funders
  • National Science Foundation
  • European Foundation for the Study of Diabetes
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (173138, 310030)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
islet organoids, amniotic epithelial cells, type 1 diabetes, islet transplantation, cell therapy, insulin-producing cells, stem cell-derived islets
MeSH terms
Amnion, Animals, Cell Survival, Diabetes Mellitus, Experimental, Diabetes Mellitus, Type 1, Epithelial Cells, Graft Survival, Humans, Insulin, Islets of Langerhans, Organoids, Streptozocin, Transplantation, Heterologous, Islets of Langerhans Transplantation, Mice, SCID, Rats, Sprague-Dawley, Spheroids, Cellular, Tissue Engineering, Regenerative Medicine, Tissue Culture Techniques, Mice, Rats, Heterografts

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