Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins
Open accessPublished version of a preprint
Abstract
Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed a complex network inside the lumen of both tubules composed of microtubule-inner proteins (MIPs). However, the functions of most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses of the Tetrahymena thermophila native doublet microtubule and identify 42 MIPs. These data shed light on the evolutionarily conserved and diversified roles of MIPs. In addition, we identified MIPs potentially responsible for the assembly and stability of the doublet outer junction. Knockout of the evolutionarily conserved outer junction component CFAP77 moderately diminishes Tetrahymena swimming speed and beat frequency, indicating the important role of CFAP77 and outer junction stability in cilia beating generation and/or regulation.
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ColabFold - Making protein folding accessible to all
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- Authors
- Kubo, S., Black, C. S., Joachimiak, E., Yang, S. K., Legal, T., Peri, K., Khalifa, A. A. Z., Ghanaeian, A., McCafferty, C. L., Valente-Paterno, M., De Bellis, C., Huynh, P. M., Fan, Z., Marcotte, E. M., Wloga, D., & Bui, K. H.
- Affiliations
- McGill University (CA)
- Instytut Biologii Doświadczalnej im. Marcelego Nenckiego (PL)
- The University of Texas at Austin (US)
- Corresponding author
- Bui, Khanh Huy
- ORCID
- Venue
- Nature Communications
- Published
- 2023-04-15
- Volume
- 14
- Issue
- 1
- Pages
- 2168-2168
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC10105768
- OpenAlex
- W4365816407
- Cited by
- 93
- References
- 7
- Citations by year
- 2026: 8 · 2025: 25 · 2024: 37 · 2023: 21 · 2022: 2
- FWCI
- 15.09
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Gouvernement du Canada | Canadian Institutes of Health Research (PJT-156354)
- Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (RGPIN-2022-04774)
- Welch Foundation (F1515)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Keywords
- doublet microtubules, ciliary axoneme, microtubule inner proteins, Tetrahymena thermophila, ciliary beating, ciliary motility, microtubule assembly, single-particle cryo-EM, cilia
- MeSH terms
- Cilia, Cytoskeleton, Microtubule Proteins, Microtubules, Tetrahymena, Tetrahymena thermophila, Cryoelectron Microscopy, Axoneme
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