Inner lumen proteins stabilize doublet microtubules in cilia and flagella
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Abstract
Motile cilia are microtubule-based organelles that play important roles in most eukaryotes. Although axonemal microtubules are sufficiently stable to withstand their beating motion, it remains unknown how they are stabilized while serving as tracks for axonemal dyneins. To address this question, we have identified two uncharacterized proteins, FAP45 and FAP52, as microtubule inner proteins (MIPs) in Chlamydomonas . These proteins are conserved among eukaryotes with motile cilia. Using cryo-electron tomography (cryo-ET) and high-speed atomic force microscopy (HS-AFM), we show that lack of these proteins leads to a loss of inner protrusions in B-tubules and less stable microtubules. These protrusions are located near the inner junctions of doublet microtubules and lack of both FAP52 and a known inner junction protein FAP20 results in detachment of the B-tubule from the A-tubule, as well as flagellar shortening. These results demonstrate that FAP45 and FAP52 bind to the inside of microtubules and stabilize ciliary axonemes.
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In Chlamydomonas or other unicellular eukaryotes, CFAP20, PACRG, CFAP45, CFAP52 and EFHC1/Rib72 are all needed to regulate ciliary beat patterns.
“Consistent with such a role, in Chlamydomonas or other unicellular eukaryotes, CFAP20, PACRG, CFAP45, CFAP52 and EFHC1/Rib72 are all needed to regulate ciliary beat patterns20,76–78.”
From The inner junction protein CFAP20 functions in motile and non-motile cilia and is critical for vision (2022) · cites this paper as [76]
The Results section on Chlamydomonas FAP45 and FAP52 mutants reports a slight reduction in beat frequency without FAP45 and impaired beating of the double mutant under viscous conditions. It also says the FAP52 single mutant was similar to wild type and that flagellar beating in the mutants appeared normal during swimming. Thus this paper supports a role for FAP45 and FAP52 in motility under some conditions, but not the claim that all five proteins are needed to regulate beat patterns. (LLM, conf=0.88)
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- Authors
- Owa, M., Uchihashi, T., Yanagisawa, H.-A., Yamano, T., Iguchi, H., Fukuzawa, H., Wakabayashi, K.-I., Ando, T., & Kikkawa, M.
- Affiliations
- The University of Tokyo (JP)
- Nagoya University (JP)
- Kyoto University (JP)
- Tokyo Institute of Technology (JP)
- Kanazawa University (JP)
- Corresponding author
- Kikkawa, Masahide
- ORCID
- Venue
- Nature Communications
- Published
- 2019-03-08
- Volume
- 10
- Issue
- 1
- Pages
- 1143-1143
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC6408466
- OpenAlex
- W2885457519
- Cited by
- 140
- References
- 61
- Citations by year
- 2026: 6 · 2025: 17 · 2024: 12 · 2023: 32 · 2022: 20 · 2021: 13 · 2020: 22 · 2019: 17 · 2018: 1
- FWCI
- 10.81
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion, cc-by)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Kyoto University Research Information Repository (Kyoto University) (submittedVersion, cc-by)
- Funders
- Japan Society for the Promotion of Science (17H06121, 25120714, #26119003, 18H01837, 16H04805, 15H01206, CREST, 16K07399)
- Japan Science and Technology Agency (CREST, JPMJAL1105)
- Core Research for Evolutional Science and Technology
- Advanced Low Carbon Technology Research and Development Program (JPMJAL1105)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Keywords
- microtubule inner proteins, doublet microtubules, motile cilia, flagella, Chlamydomonas
- MeSH terms
- Cilia, Flagella, Gene Expression, Chlamydomonas reinhardtii, Microscopy, Atomic Force, Cryoelectron Microscopy, Algal Proteins, Axoneme, Electron Microscope Tomography, Axonemal Dyneins
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