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Cited by 26Bacterial Genetics and BiotechnologyCellular transport and secretionBiochemical and Structural Characterization

TerC proteins function during protein secretion to metalate exoenzymes

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He, Bixi, Sachla, Ankita J., Helmann, John D.·2023·Nature Communications
DOI 10.1038/s41467-023-41896-1PMC PMC10550928

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Abstract

Cytosolic metalloenzymes acquire metals from buffered intracellular pools. How exported metalloenzymes are appropriately metalated is less clear. We provide evidence that TerC family proteins function in metalation of enzymes during export through the general secretion (Sec-dependent) pathway. Bacillus subtilis strains lacking MeeF(YceF) and MeeY(YkoY) have a reduced capacity for protein export and a greatly reduced level of manganese (Mn) in the secreted proteome. MeeF and MeeY copurify with proteins of the general secretory pathway, and in their absence the FtsH membrane protease is essential for viability. MeeF and MeeY are also required for efficient function of the Mn 2+ -dependent lipoteichoic acid synthase (LtaS), a membrane-localized enzyme with an extracytoplasmic active site. Thus, MeeF and MeeY, representative of the widely conserved TerC family of membrane transporters, function in the co-translocational metalation of Mn 2+ -dependent membrane and extracellular enzymes.

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  • The bulk of the quota for manganese and iron is devoted to intracellular enzymes, with a variable fraction partitioned to the membrane or to extracellular enzymes, and the latter may require dedicated maturation pathways.

    “The bulk of the quota for manganese and iron is devoted to intracellular enzymes (Figure 3), with a variable fraction partitioned to the membrane (e.g., respiratory complexes) or to extracellular enzymes. The latter may require dedicated pathways for their maturation.95,96”

    From Metals in Motion: Understanding Labile Metal Pools in Bacteria (2025) · cites this paper as [95]

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Authors
He, B., Sachla, A. J., & Helmann, J. D.
Affiliations
Cornell University (US)
Corresponding author
Helmann, John D.
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2023-10-04
Volume
14
Issue
1
Pages
6186-6186
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-023-41896-1
PMC
PMC10550928
OpenAlex
W4387343660
Cited by
26
References
4
Citations by year
2026: 7 · 2025: 8 · 2024: 11
FWCI
4.13
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • PubMed Central (submittedVersion, cc-by-nc)
  • PubMed Central (submittedVersion, cc-by)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
U.S. Department of Health & Human Services | National Institutes of Health (R35GM122461)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords
MEEF, manganese, general secretion pathway, Sec-dependent secretion, LTA, Bacillus subtilis, membrane transporters

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