Fluorogenic CRISPR for genomic DNA imaging
Open accessAbstract
Genomic DNA exhibits high heterogeneity in terms of its dynamic within the nucleus, its structure and functional roles. CRISPR-based imaging approaches can image genomic loci in living cells. However, conventional CRISPR-based tools involve expressing constitutively fluorescent proteins, resulting in high background and nonspecific nucleolar signal. Here, we construct fluorogenic CRISPR (fCRISPR) to overcome these issues. fCRISPR is designed with dCas9, an engineered sgRNA, and a fluorogenic protein. Fluorogenic proteins are degraded unless they are bound to specific RNA hairpins. These hairpins are inserted into sgRNA, resulting in dCas9: sgRNA: fluorogenic protein ternary complexes that enable fluorogenic DNA imaging. With fCRISPR, we image various genomic DNA in different human cells with high signal-to-noise ratio and sensitivity. Furthermore, fCRISPR tracks chromosomes dynamics and length. fCRISPR also allows DNA double-strand breaks (DSBs) and repair to be tracked in real time. Taken together, fCRISPR offers a high-contrast and sensitive platform for imaging genomic loci.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Evolved base editor variants such as EvoFERNY and CBE6-variants no longer have the GC sequence context restriction.
“The evolved variants such as EvoFERNY and CBE6-variants do no longer have this sequence context.”
From Protocol to create isogenic disease models from adult stem cell-derived organoids using next-generation CRISPR tools (2024) · cites this paper as [26]
The Introduction discusses CRISPR-based genomic imaging, and Methods > Plasmids construction describes guide-RNA design; neither addresses evolved base editors. Searches for EvoFERNY, CBE6 variants, and GC sequence-context restrictions found no passage in our copy that speaks to the claim. No page numbers were available for these passages. (LLM, conf=0.76)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339 · see citing works
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Konermann, S., Brigham, M. D., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., Nishimasu, H., et al. (2015) · Nature · doi:10.1038/nature14136 · Cited by 2,825 · cited 1× · see citing works
“We found that fCRISPR performed on par with the conventional CRISPR imaging systems for chromosomal dynamics imaging (Supplementary Fig. and Supplementary Movie 2 – 4).”
Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System
Chen, B., Gilbert, L. A., Cimini, B. A., Schnitzbauer, J., Zhang, W., Li, G., Park, J. S., Blackburn, E. H., Weissman, J. S., Qi, L. S., et al. (2013) · Cell · doi:10.1016/j.cell.2013.12.001 · Cited by 1,931 · see citing works
RNA Mimics of Green Fluorescent Protein
Paige, J. S., Wu, K., & Jaffrey, S. R. (2011) · Science · doi:10.1126/science.1207339 · Cited by 1,353 · see citing works
Self-citationHeterogeneity in telomere length of human chromosomes
Lansdorp, P. M. (1996) · Human Molecular Genetics · doi:10.1093/hmg/5.5.685 · Cited by 879 · see citing works
the 4D Nucleome Network, Dekker, J., Belmont, A. S., Guttman, M., Leshyk, V. O., Lis, J. T., Lomvardas, S., Mirny, L. A., O’Shea, C. C., Park, P. J., et al. (2017) · Nature · doi:10.1038/nature23884 · Cited by 732 · see citing works
TNF and IL-1 exhibit distinct ubiquitin requirements for inducing NEMO–IKK supramolecular structures
Tarantino, N., Tinévez, J.-Y., Crowell, E. F., Boisson, B., Henriques, R., Mhlanga, M. M., Agou, F., Israël, A., & Laplantine, E. (2014) · The Journal of Cell Biology · doi:10.1083/jcb.201307172 · Cited by 478 · see citing works
Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow
Ma, H., Tu, L., Naseri, A., Huisman, M., Zhang, S., Grünwald, D., & Pederson, T. (2016) · Nature Biotechnology · doi:10.1038/nbt.3526 · Cited by 472 · see citing works
An improved MS2 system for accurate reporting of the mRNA life cycle
Tutucci, E., Vera, M., Biswas, J., Garcia, J. F., Parker, R., & Singer, R. H. (2017) · Nature Methods · doi:10.1038/nmeth.4502 · Cited by 332 · see citing works
Spatial Dynamics of Chromosome Translocations in Living Cells
Roukos, V., Voss, T. C., Schmidt, C. K., Lee, S., Wangsa, D., & Misteli, T. (2013) · Science · doi:10.1126/science.1237150 · Cited by 312 · see citing works
CRISPR-mediated live imaging of genome editing and transcription
Wang, H., Nakamura, M., Abbott, T. R., Zhao, D., Luo, K., Yu, C., Nguyen, C. M., Lo, A., Daley, T., Russa, M. F. L., et al. (2019) · Science · doi:10.1126/science.aax7852 · Cited by 292 · see citing works
Self-citationCASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells
Deng, W., Shi, X., Tjian, R., Lionnet, T., & Singer, R. H. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1515692112 · Cited by 273 · see citing works
Imaging RNA polymerase III transcription using a photostable RNA–fluorophore complex
Song, W., Filonov, G. S., Kim, H., Hirsch, M., Li, X., Moon, J. D., & Jaffrey, S. R. (2017) · Nature Chemical Biology · doi:10.1038/nchembio.2477 · Cited by 273 · see citing works
Self-citationLive cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9
Qin, P., Parlak, M., Kuscu, C., Bandaria, J. N., Mir, M., Szlachta, K. A., Singh, R., Darzacq, X., Yıldız, A., & Adli, M. (2017) · Nature Communications · doi:10.1038/ncomms14725 · Cited by 234 · see citing works
CRISPR-Cas9 nuclear dynamics and target recognition in living cells
Ma, H., Tu, L., Naseri, A., Huisman, M., Zhang, S., Grünwald, D., & Pederson, T. (2016) · The Journal of Cell Biology · doi:10.1083/jcb.201604115 · Cited by 216 · see citing works
In-Gel Imaging of RNA Processing Using Broccoli Reveals Optimal Aptamer Expression Strategies
Filonov, G. S., Kam, C. W., Song, W., & Jaffrey, S. R. (2015) · Chemistry & Biology · doi:10.1016/j.chembiol.2015.04.018 · Cited by 198 · see citing works
Self-citationLiu, Y., Zou, R. S., He, S., Nihongaki, Y., Li, X., Razavi, S., Wu, B., & Ha, T. (2020) · Science · doi:10.1126/science.aay8204 · Cited by 196 · see citing works
Self-citationCRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging
Ma, H., Tu, L., Naseri, A., Chung, Y.-C., Grünwald, D., Zhang, S., & Pederson, T. (2018) · Nature Methods · doi:10.1038/s41592-018-0174-0 · Cited by 173 · see citing works
Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling
Cheng, A. W., Jillette, N., Lee, P. L., Plaskon, D. M., Fujiwara, Y., Wang, W., Taghbalout, A., & Wang, H. (2016) · Cell Research · doi:10.1038/cr.2016.3 · Cited by 164 · see citing works
Self-citationLong-term dual-color tracking of genomic loci by modified sgRNAs of the CRISPR/Cas9 system
Shao, S., Zhang, W., Hu, H., Xue, B., Qin, J., Sun, C., Sun, Y., Wei, W., & Sun, Y. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw066 · Cited by 164 · see citing works
Li, X., Kim, H., Litke, J. L., Wu, J., & Jaffrey, S. R. (2019) · Angewandte Chemie International Edition · doi:10.1002/anie.201914576 · Cited by 126 · see citing works
Self-citationLive imaging of mRNA using RNA-stabilized fluorogenic proteins
Wu, J., Zaccara, S., Khuperkar, D., Kim, H., Tanenbaum, M. E., & Jaffrey, S. R. (2019) · Nature Methods · doi:10.1038/s41592-019-0531-7 · Cited by 116 · see citing works
Self-citationRepurposing an adenine riboswitch into a fluorogenic imaging and sensing tag
Dey, S. K., Filonov, G. S., Olarerin-George, A. O., Jackson, B. T., Finley, L. W. S., & Jaffrey, S. R. (2021) · Nature Chemical Biology · doi:10.1038/s41589-021-00925-0 · Cited by 109 · see citing works
Self-citationChromosomal instability in osteosarcoma and its association with centrosome abnormalities
Al-Romaih, K. I., Bayani, J., Vorobyova, J., Karásková, J., Park, P. C., Zieleńska, M., & Squire, J. A. (2003) · Cancer Genetics and Cytogenetics · doi:10.1016/s0165-4608(02)00929-9 · Cited by 85 · see citing works
Li, X., Mo, L., Litke, J. L., Dey, S. K., Suter, S. R., & Jaffrey, S. R. (2020) · Journal of the American Chemical Society · doi:10.1021/jacs.0c02931 · Cited by 84 · see citing works
Self-citationLyu, X.-Y., Deng, Y., Huang, X., Li, Z., Fang, G.-Q., Dong, Y., Wang, F.-L., Kang, W., Shen, E.-Z., & Song, C. (2022) · Cell Research · doi:10.1038/s41422-022-00712-z · Cited by 69 · see citing works
Self-citationEnhanced DNA repair through droplet formation and p53 oscillations
Heltberg, M. L., Lucchetti, A., Hsieh, F., Nguyen, D. P. M., Chen, S., & Jensen, M. H. (2022) · Cell · doi:10.1016/j.cell.2022.10.004 · Cited by 55 · see citing works
Self-Assembly of Intracellular Multivalent RNA Complexes Using Dimeric Corn and Beetroot Aptamers
Wu, J., Svensen, N., Song, W., Kim, H., Zhang, S., Li, X., & Jaffrey, S. R. (2022) · Journal of the American Chemical Society · doi:10.1021/jacs.1c13583 · Cited by 50 · see citing works
Self-citationZou, R. S., Marín-González, A., Liu, Y., Liu, H. B., Shen, L. L., Dveirin, R. K., Luo, J. X. J., Kalhor, R., & Ha, T. (2022) · Nature Cell Biology · doi:10.1038/s41556-022-00975-z · Cited by 48 · see citing works
High-throughput single-molecule telomere characterizationPreprint
McCaffrey, J., YOUNG, E. A., Lassahn, K. A., Sibert, J. G., Pastor, S. J., Riethman, H. C., & Xiao, M. (2017) · Genome Research · doi:10.1101/gr.222422.117 · Cited by 46 · see citing works
The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold
Truong, L., Kooshapur, H., Dey, S. K., Li, X., Tjandra, N., Jaffrey, S. R., & Ferré-D’Amaré, A. R. (2021) · Nature Chemical Biology · doi:10.1038/s41589-021-00931-2 · Cited by 41 · see citing works
Self-citationEngineering Fluorophore Recycling in a Fluorogenic RNA Aptamer
Li, X., Wu, J., & Jaffrey, S. R. (2021) · Angewandte Chemie International Edition · doi:10.1002/anie.202108338 · Cited by 38 · see citing works
Self-citationNaturally occurring three-way junctions can be repurposed as genetically encoded RNA-based sensors
Moon, J. D., Wu, J., Dey, S. K., Litke, J. L., Li, X., Kim, H., & Jaffrey, S. R. (2021) · Cell chemical biology · doi:10.1016/j.chembiol.2021.04.022 · Cited by 30 · see citing works
Self-citationDeshpande, R. A., Marín-González, A., Barnes, H. K., Woolley, P. R., Ha, T., & Paull, T. T. (2023) · Nature Communications · doi:10.1038/s41467-023-41544-8 · Cited by 26 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Engineered CRISPR/Cas Ribonucleoproteins for Enhanced Biosensing and Bioimaging
Cao, L., Wang, Z., Lei, C., & Nie, Z. (2025) · Analytical Chemistry · doi:10.1021/acs.analchem.4c06789 · Cited by 56 · see citing works
Aptamers in bioanalytical chemistry: current trends in development and application
Zhang, L., Li, Y., Sun, J., Zhao, Y., Xia, F., Huang, F., Liu, Y., Mao, X., Wang, S., Zuo, X., et al. (2025) · Science China Chemistry · doi:10.1007/s11426-025-2968-x · Cited by 32 · see citing works
Self-citationNear-infrared fluorogenic RNA for in vivo imaging and sensing
Chen, Z., Chen, W., Xu, C., Song, H., Ji, X., Jiang, H., Duan, H., Li, Z., Gao, W., Yao, T., et al. (2025) · Nature Communications · doi:10.1038/s41467-024-55093-1 · Cited by 35 · see citing works
Self-citationZhou, S., Ran, J., Man, S., Zhang, J., Yuan, R., & Yang, X. (2024) · Analytical Chemistry · doi:10.1021/acs.analchem.4c01347 · Cited by 28 · see citing works
Chen, M., Huang, X., Shi, Y., Wang, W., Huang, Z., Tong, Y., Zou, X., Xu, Y., & Dai, Z. (2024) · Advanced Science · doi:10.1002/advs.202402534 · Cited by 22 · see citing works
Unlocking the Potential of Chemically Modified Nucleic Acid Therapeutics
Gao, J., Nutan, B., Gargouri, D., Pisal, N. D., Do, V., Zubair, M., Alanzi, H., Wang, H., Lee, D., Joshi, N., et al. (2024) · Advanced Therapeutics · doi:10.1002/adtp.202400231 · Cited by 20 · see citing works
Self-citationCelotti, M., Derks, L. L. M., van Es, J., van Boxtel, R., Clevers, H., & Geurts, M. H. (2024) · STAR Protocols · doi:10.1016/j.xpro.2024.103189 · Cited by 17 · see citing works
Kuffner, C. J., Marzilli, A. M., & Ngo, J. T. (2025) · Nature Methods · doi:10.1038/s41592-025-02782-4 · Cited by 16 · see citing works
Zhang, L., Zhang, Z., Yang, Z., Wang, S., Ruifang, L., Zhu, L., Li, C., Xie, L., Ge, S., & Wu, J. (2024) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2024.137140 · Cited by 12 · see citing works
Self-citationLive genome imaging by CRISPR engineering: progress and problems
Park, E.-J., & Kim, H. (2025) · Experimental & Molecular Medicine · doi:10.1038/s12276-025-01498-x · Cited by 11 · see citing works
Visualizing DNA/RNA, Proteins, and Small Molecule Metabolites within Live Cells.
Jia, D., Cui, M.-H., & Ding, X.-T. (2024) · Small · doi:10.1002/smll.202404482 · Cited by 8 · see citing works
CRISPR-Based Live Cell Biosensing: Innovations in Signal Labeling Strategies and Delivery Systems
Yin, Y., Li, Z., Liu, C., Zhang, X., & Ou, Q. (2025) · ACS Sensors · doi:10.1021/acssensors.5c01973 · Cited by 9 · see citing works
Self-citationLiu, M., Huang, K., Zhang, J., Li, Q., Wang, X., Gu, B.-M., Tang, H., Du, Z., Hu, L., Qi, S., et al. (2025) · Nature Biotechnology · doi:10.1038/s41587-025-02887-3 · Cited by 11 · see citing works
Self-citationCRISPR/Cas bioimaging: From whole body biodistribution to single-cell dynamics
Kolesova, E. P., Pulone, S., Kostyushev, D. S., & Tasciotti, E. (2025) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2025.115619 · Cited by 8 · see citing works
RNA-Based Fluorescent Sensor with RhoBAST
Song, H., Reheman, Z., Fang, Y., Jiang, H., Hou, R., Shi, Y., Weng, Y., Li, X., & Liu, L. (2025) · Analytical Chemistry · doi:10.1021/acs.analchem.5c02623 · Cited by 7 · see citing works
Self-citationFluorogenic RNA aptamer based artificial membraneless organelles for small molecule and cell sensing
Wang, L., Ji, R., Yan, J., Ma, Y., Zhou, S., Chen, H., & Ren, K. (2024) · Chemical Engineering Journal · doi:10.1016/j.cej.2024.155935 · Cited by 6 · see citing works
Setia, A., Patil, D., Randhave, N. V., Vaishali, Verma, N., Rani, K., Kale, A., Wagh, B., Kumar, V., Malik, A. K., et al. (2026) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2026.150582 · Cited by 7 · see citing works
A Guide to Guides: An Overview of SpCas9 sgRNA Scaffold Variants and Modifications
Saeger, J. D. (2025) · SynBio · doi:10.3390/synbio3040019 · Cited by 5 · see citing works
Choi, M. Y., Li, C., Choi, J., & Choi, J. (2025) · Chemical Communications · doi:10.1039/d5cc05016b · Cited by 5 · see citing works
Ultrasensitive and visual detection of pseudorabies virus based on CRISPR-Cas12b system
Kang, H., Yang, X., Jiang, R., Gao, P., Zhang, Y., Zhou, L., Ge, X., Han, J., Guo, X., & Yang, H. (2025) · Microbial Pathogenesis · doi:10.1016/j.micpath.2025.107447 · Cited by 5 · see citing works
Feng, L., Huang, T., Han, Y., Meng, T., Wang, D., Mei, Z., Ma, Y., Ma, Y., Li, J., & Zhang, R. (2025) · Advanced Science · doi:10.1002/advs.202513014 · Cited by 4 · see citing works
Cytotransducers for Visualization of Spatiotemporal Intercellular Communication
Kimura, N., Yamanishi, Y., & Sakuma, S. (2025) · Small · doi:10.1002/smll.202503749 · Cited by 3 · see citing works
Zhao, G., Li, J., Fu, S., Wang, A., Meng, X., Dong, H., & Zhang, X. (2025) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2025.118639 · Cited by 3 · see citing works
Engineering Guide RNAs for CRISPR-Based Biosensors
Liu, X., Chen, T., Li, X. M., Li, Q., Zou, G., Fan, W., & Liu, C. (2026) · ACS Sensors · doi:10.1021/acssensors.6c01763 · Cited by 3 · see citing works
Self-citationParasite genome organization: come together, right now!
Singh, P., Walther, L., Cabahug, M. D., Faria, J. R. C., & Bryant, J. M. (2025) · Trends in Parasitology · doi:10.1016/j.pt.2025.07.010 · Cited by 3 · see citing works
Illuminating the genome: emerging approaches in CRISPR-Cas live-cell imaging
Xiao, Z., & Sun, Y. (2026) · Nucleic Acids Research · doi:10.1093/nar/gkaf1540 · Cited by 3 · see citing works
Closing the loops: chromatin loop dynamics after DNA damage
Vidi, P., Liu, J., Bonin, K., & Bloom, K. (2024) · Nucleus · doi:10.1080/19491034.2024.2438633 · Cited by 2 · see citing works
Photoactivatable RNA Tags for Subcellular Photolabeling of RNA
Chen, Z., Jiang, H., Yuan, L., Yao, T., Rong, X., Gao, W., Zeng, C., He, L., Yin, Y., Jaffrey, S. R., et al. (2025) · Journal of the American Chemical Society · doi:10.1021/jacs.5c07380 · Cited by 2 · see citing works
Self-citationExpanding the CRISPR/Cas toolkit: applications in proteomics and theranostics
Punde, A., Dey, S., Pandire, R., Bhattacharjee, A., & Patra, C. (2025) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2025.1713700 · Cited by 2 · see citing works
Zhu, Y. (2025) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2025.110799 · Cited by 2 · see citing works
CRISPR–Cas-based live cell imaging of genome dynamics
Zhu, Y., Moerner, W. E., & Qi, L. S. (2026) · Nature Reviews Genetics · doi:10.1038/s41576-026-00949-z · Cited by 3 · see citing works
Zou, G., Liu, X., Li, Y., Li, X., Wang, J., He, X., Zhu, T., & Liu, C. (2026) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2026.118981 · Cited by 2 · see citing works
Self-citationUnveiling the invisible genomic dynamics
Kwon, J., Park, Y., & Ha, T. (2025) · Experimental & Molecular Medicine · doi:10.1038/s12276-025-01434-z · Cited by 2 · see citing works
Jiang, Y., Zhao, C., Zhang, C., Li, W., Liu, D., & Zhao, B. (2024) · Biophysics Reports · doi:10.52601/bpr.2024.240043 · Cited by 1 · see citing works
Visualizing Nucleic Acid Loci with the CRISPR-Cas System: Current Approaches
Abushinova, G. A., Zherdeva, V. V., Vassina, E. M., & Maloshenok, L. G. (2024) · Journal of Biomedical Photonics & Engineering · doi:10.18287/jbpe24.10.040203 · Cited by 1 · see citing works
3D Genome Engineering: Current Advances and Therapeutic Opportunities in Human Diseases
Jiang, X., Wang, X., Shen, S., Hou, S., & Chen, Y. (2025) · Research · doi:10.34133/research.0865 · Cited by 2 · see citing works
Yu, Q., Xie, J., & Zhen, S. J. (2025) · Biosensors · doi:10.3390/bios15100700 · Cited by 1 · see citing works
Self-citationVisualizing genetic information flow in space and time
Chen, K., Zhao, K., & Wan, Y. (2025) · Science China Life Sciences · doi:10.1007/s11427-025-3158-6 · Cited by 1 · see citing works
Fluorogenic CRISPR for DNA imaging in live mammalian cells
Wan, W., Ji, X., Song, H., Zhang, Z., Kwok, C. K., Fang, X., & Li, X. (2026) · Cell chemical biology · doi:10.1016/j.chembiol.2025.10.013 · Cited by 1 · see citing works
Self-citationProgrammable, target-induced fluorogenic CRISPR–tDeg platform for live-cell RNA visualization
Zhong, H., Zhou, J., Qin, F., Zhang, X., & Chen, M. (2026) · Nucleic Acids Research · doi:10.1093/nar/gkag279 · Cited by 1 · see citing works
Wu, Y., Zhou, S., Ye, X., Zhu, T., Deng, F., & Yang, D. (2026) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2026.118893 · Cited by 1 · see citing works
Nuclear mechanotransduction: tools for mechanical perturbation and chromatin characterization
Soto, J., Origer, N., Wu, Y. N., Cardenas‐Benitez, B., Massad, R. N., Rahgoshay, D., Lee, A., Downing, T., & Li, S. (2026) · Nucleus · doi:10.1080/19491034.2026.2690847 · Cited by 1 · see citing works
CRISPR-based live-cell DNA imaging: Technologies, biological insights and future perspectives
Liu, Y., Feng, L., Li, J., & Zhang, R. (2026) · Biotechnology Advances · doi:10.1016/j.biotechadv.2026.108999 · Cited by 1 · see citing works
Gao, W., Chen, Z., Rong, X., & Li, X. (2024) · Scientia Sinica Vitae · doi:10.1360/ssv-2024-0001 · Cited by 1 · see citing works
Self-citationA Conditional Degron Tag for RNA Sensing in BacteriaPreprint
Simons, T. J., & Hammond, M. C. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.12.16.628724
Research progress of chromatin imaging and manipulation
Liu, M., Li, Q., Ma, Y., Tang, H., Huang, K., & Wang, H. (2025) · Scientia Sinica Vitae · doi:10.1360/ssv-2025-0105
Self-citationDevelopment and application of fluorescence RNA imaging tools
Yin, P., Wang, J., Huang, C., KUANG, S., & Nie, Z. (2025) · Scientia Sinica Vitae · doi:10.1360/ssv-2024-0300
Imaging genome dynamics in real time with CRISPR-based technologies
Adams, B., & Wu, J. (2026) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2026.102700
Self-citationhnRNPK condensates facilitate enhancer–promoter looping and RNA polymerase II recruitment
Ye, R., Zhao, H., Chen, J., Wu, Q., Wang, D., Cai, Z., Wang, R., Li, J., Cheng, T., Zhang, M., et al. (2026) · Nature Genetics · doi:10.1038/s41588-026-02710-y
Self-citationQin, Y., Zhang, G., & Hu, Y. (2026) · ACS Sensors · doi:10.1021/acssensors.6c01856
Yang, Y., Guo, Y., An, H., Peng, Y., Qi, H., Peng, W., 满淑丽, & Ma, L. (2026) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2026.119178
- Authors
- Zhang, Z., Rong, X., Xie, T., Li, Z., Song, H., Zhen, S., Wang, H., Wu, J., Jaffrey, S. R., & Li, X.
- Affiliations
- Chinese Academy of Sciences (CN)
- Southwest University (CN)
- Center for Life Sciences (CN)
- University of Massachusetts Amherst (US)
- Cornell University (US)
- Corresponding author
- Li, Xing
- Venue
- Nature Communications
- Published
- 2024-01-31
- Volume
- 15
- Issue
- 1
- Pages
- 934-934
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC10831063
- OpenAlex
- W4391387015
- Cited by
- 51
- References
- 34
- Citations by year
- 2026: 14 · 2025: 25 · 2024: 12
- FWCI
- 6.94
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- The Talent research start-up fund, Beijing Institutes of Life Science, Chinese Academy of Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- dCas9, RNA hairpin, chromosome dynamics, chromosome length, DNA double-strand breaks, DNA repair, live-cell imaging
- MeSH terms
- RNA, Guide, CRISPR-Cas Systems, DNA, Humans, Genome, Genomics, CRISPR-Cas Systems
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
