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Cited by 44Polysaccharides and Plant Cell WallsGlycosylation and Glycoproteins ResearchAdvanced NMR Techniques and Applications

Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall

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Poulhazan, Alexandre, Arnold, Alexandre A., Mentink-Vigier, Frederic, Muszyński, Artur, Azadi, Parastoo, Halim, Adnan, Vakhrushev, Sergey Y., Joshi, Hiren Jitendra +3 more·2024·Nature Communications
DOI 10.1038/s41467-024-45246-7PMC PMC10837150

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Abstract

Microalgae are a renewable and promising biomass for large-scale biofuel, food and nutrient production. However, their efficient exploitation depends on our knowledge of the cell wall composition and organization as it can limit access to high-value molecules. Here we provide an atomic-level model of the non-crystalline and water-insoluble glycoprotein-rich cell wall of Chlamydomonas reinhardtii . Using in situ solid-state and sensitivity-enhanced nuclear magnetic resonance, we reveal unprecedented details on the protein and carbohydrate composition and their nanoscale heterogeneity, as well as the presence of spatially segregated protein- and glycan-rich regions with different dynamics and hydration levels. We show that mannose-rich lower-molecular-weight proteins likely contribute to the cell wall cohesion by binding to high-molecular weight protein components, and that water provides plasticity to the cell-wall architecture. The structural insight exemplifies strategies used by nature to form cell walls devoid of cellulose or other glycan polymers.

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  • AsymPolPOK and other biradicals are small, water-soluble molecules that diffuse through porous cell walls without disrupting their structure.

    “AsymPolPOK and other biradicals are small (2–3 nm), water-soluble molecules that diffuse through porous cell walls without disrupting their structure, enabling widespread use in the structural analysis of plant, fungal, algal, and bacterial cell wall materials as well as intact mammalian cells84–90.”

    From Distinct echinocandin responses of Candida albicans and Candida auris cell walls revealed by solid-state NMR (2025) · cites this paper as [86]

    The Methods (page unavailable) describe an aqueous AMUPol biradical solution applied to extracted algal cell walls and intact microalgae. The Results (page unavailable) suggest that the polarizing radical localizes in the cell wall, but do not establish diffusion through pores or that the biradical leaves wall structure undisturbed. The copy does not give a 2–3 nm size, identify AsymPolPOK as the reagent used, or establish use across the other listed materials and cells. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

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Authors
Poulhazan, A., Arnold, A. A., Mentink-Vigier, F., Muszyński, A., Azadi, P., Halim, A., Vakhrushev, S. Y., Joshi, H. J., Wang, T., Warschawski, D. E., & Marcotte, I.
Affiliations
  • Université du Québec à Montréal (CA)
  • University of Georgia (US)
  • University of Copenhagen (DK)
  • Michigan State University (US)
  • Centre National de la Recherche Scientifique (FR)
Corresponding author
  • Wang, Tuo
  • Warschawski, Dror E.
  • Marcotte, Isabelle
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2024-02-02
Volume
15
Issue
1
Pages
986-986
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-024-45246-7
PMC
PMC10837150
OpenAlex
W4391476405
Cited by
44
References
0
Citations by year
2026: 16 · 2025: 21 · 2024: 7
FWCI
10.68
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, cc-by)
  • HAL (submittedVersion, other-oa)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
Funders
  • Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (RGPIN-2018–06200)
  • National Science Foundation (NSF/DMR-1644779)
  • U.S. Department of Health & Human Services | National Institutes of Health (P41 GM122698, S10 OD018519)
  • U.S. Department of Energy (DE-SC0015662)
  • DOE | Office of Energy Efficiency & Renewable Energy | Wind Energy Technologies Office (U.S. Department of Energy&apos;s (DE-SC0023702)
  • Centre National de la Recherche Scientifique (UMR 7203)
  • Danmarks Grundforskningsfond (DNRF107)
  • Novo Nordisk Fonden (NNF22OC0076899)
  • Villum Fonden (00025438)
  • Mizutani Foundation for Glycoscience
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
  • Physical Sciences › Chemistry › Spectroscopy › Advanced NMR Techniques and Applications
Keywords
Chlamydomonas reinhardtii, cell wall plasticity, cell wall composition
MeSH terms
Cell Wall, Cellulose, Chlamydomonas, Glycoproteins, Water, Chlamydomonas reinhardtii

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