Cited by 237Hydrogels: synthesis, properties, applications3D Printing in Biomedical ResearchCellular Mechanics and Interactions

The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.

Synthetic dynamic hydrogels promote degradation-independent in vitro organogenesis

Open access

Chrisnandy, Antonius, Blondel, Delphine, Rezakhani, Saba, Broguiere, Nicolas, Lutolf, Matthias P.·2022·Nature Materials

DOI 10.1038/s41563-021-01136-7

Open this paper in CoChat — free account

Abstract

Epithelial organoids are most efficiently grown from mouse-tumour-derived, reconstituted extracellular matrix hydrogels, whose poorly defined composition, batch-to-batch variability and immunogenicity limit clinical applications. Efforts to replace such ill-defined matrices for organoid culture have largely focused on non-adaptable hydrogels composed of covalently crosslinked hydrophilic macromolecules. However, the excessive forces caused by tissue expansion in such elastic gels severely restrict organoid growth and morphogenesis. Chemical or enzymatic degradation schemes can partially alleviate this problem, but due to their irreversibility, long-term applicability is limited. Here we report a family of synthetic hydrogels that promote extensive organoid morphogenesis through dynamic rearrangements mediated by reversible hydrogen bonding. These tunable matrices are stress relaxing and thus promote efficient crypt budding in intestinal stem-cell epithelia through increased symmetry breaking and Paneth cell formation dependent on yes-associated protein 1. As such, these well-defined gels provide promising versatile matrices for fostering elaborate in vitro morphogenesis.

Integrity signals

Registry

Crossref · 10.1038/s41563-021-01136-7

Cites

53 of 53 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
Sato, T., Vries, R. G., Snippert, H. J., van de Wetering, M., Barker, N., Stange, D. E., van Es, J. H., Abo, A., Kujala, P., Peters, P. J., et al. (2009) · Nature · doi:10.1038/nature07935 · Cited by 7,204
Modeling Development and Disease with Organoids
Clevers, H. (2016) · Cell · doi:10.1016/j.cell.2016.05.082 · Cited by 3,196
Organogenesis in a dish: Modeling development and disease using organoid technologies
Lancaster, M. A., & Knoblich, J. A. (2014) · Science · doi:10.1126/science.1247125 · Cited by 2,931
Hydrogels with tunable stress relaxation regulate stem cell fate and activity
Chaudhuri, O., Gu, L., Klumpers, D., Darnell, M., Bencherif, S. A., Weaver, J. C., Huebsch, N., Lee, H.-P., Lippens, E., Duda, G. N., et al. (2016) · Nature Materials · doi:10.1038/nmat4489 · Cited by 2,443
Mechanobiology of YAP and TAZ in physiology and disease
Panciera, T., Azzolin, L., Cordenonsi, M., & Piccolo, S. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.87 · Cited by 1,461
Designer matrices for intestinal stem cell and organoid culture
Gjorevski, N., Sachs, N., Manfrin, A., Giger, S., Bragina, M. E., Ordóñez-Morán, P., Clevers, H., & Lutolf, M. P. (2016) · Nature · doi:10.1038/nature20168 · Cited by 1,426
Progress and potential in organoid research
Rossi, G., Manfrin, A., & Lutolf, M. P. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0051-9 · Cited by 1,182
Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications
Sato, T., & Clevers, H. (2013) · Science · doi:10.1126/science.1234852 · Cited by 1,170
Substrate stress relaxation regulates cell spreading
Chaudhuri, O., Gu, L., Darnell, M., Klumpers, D. D., Bencherif, S. A., Weaver, J. C., Huebsch, N., & Mooney, D. J. (2015) · Nature Communications · doi:10.1038/ncomms7365 · Cited by 903
3D Printing of Highly Stretchable and Tough Hydrogels into Complex, Cellularized Structures
Hong, S., Sycks, D. G., Chan, H. F., Lin, S., López, G. P., Guilak, F., Leong, K. W., & Zhao, X. (2015) · Advanced Materials · doi:10.1002/adma.201501099 · Cited by 890

Show all 53 references

Sorted by citations

Organoids
Zhao, Z., Chen, X., Dowbaj, A. M., Sljukic, A., Bratlie, K., Lin, L., Fong, E. L. S., Balachander, G. M., Chen, Z., Soragni, A., et al. (2022) · Nature Reviews Methods Primers · doi:10.1038/s43586-022-00174-y · Cited by 1,082
Cell–extracellular matrix mechanotransduction in 3D
Saraswathibhatla, A., Indana, D., & Chaudhuri, O. (2023) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-023-00583-1 · Cited by 887
Chemical strategies to engineer hydrogels for cell culture
Lou, J., & Mooney, D. J. (2022) · Nature Reviews Chemistry · doi:10.1038/s41570-022-00420-7 · Cited by 407
Engineered hydrogels for mechanobiology
Blache, U., Ford, E. M., Ha, B. H., Rijns, L., Chaudhuri, O., Dankers, P. Y. W., Kloxin, A. M., Snedeker, J. G., & Gentleman, E. (2022) · Nature Reviews Methods Primers · doi:10.1038/s43586-022-00179-7 · Cited by 242
Mechanical Regulation of Polymer Gels
Xu, C., Chen, Y., Zhao, S., Li, D., Tang, X., Zhang, H., Huang, J., Guo, Z., & Liu, W. (2024) · Chemical Reviews · doi:10.1021/acs.chemrev.3c00498 · Cited by 156
Organoids in gastrointestinal diseases: From experimental models to clinical translation
Günther, C., Winner, B., Neurath, M. F., & Stappenbeck, T. S. (2022) · Gut · doi:10.1136/gutjnl-2021-326560 · Cited by 145
Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions
Asim, S., Tabish, T. A., Liaqat, U., Ozbolat, I. T., & Rizwan, M. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202203148 · Cited by 141
Photoinduced Dithiolane Crosslinking for Multiresponsive Dynamic Hydrogels
Nelson, B. R., Kirkpatrick, B. E., Miksch, C. E., Davidson, M. D., Skillin, N. P., Hach, G. K., Khang, A., Hummel, S. N., Fairbanks, B. D., Burdick, J. A., et al. (2023) · Advanced Materials · doi:10.1002/adma.202211209 · Cited by 128
Recent advances in defined hydrogels in organoid research
Gan, Z., Qin, X., Liu, H., Liu, J., & Qin, J. (2023) · Bioactive Materials · doi:10.1016/j.bioactmat.2023.06.004 · Cited by 138
Viscoelastic Biomaterials for Tissue Regeneration
Wu, D. T. S., Jeffreys, N. C., Diba, M., & Mooney, D. J. (2022) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2022.0040 · Cited by 126
Imparting Ultrahigh Strength to Polymers via a New Concept Strategy of Construction of up to Duodecuple Hydrogen Bonding among Macromolecular Chains
Wen, Y. W., Li, M., Fan, L. F., Rong, M. Z., & Zhang, M. Q. (2024) · Advanced Materials · doi:10.1002/adma.202406574 · Cited by 104
Tumor organoid-immune co-culture models: exploring a new perspective of tumor immunity
Wang, J., Tao, X., Zhu, J., Dai, Z., Du, Y., Xie, Y., Chu, X., Fu, G., & Lei, Z. (2025) · Cell Death Discovery · doi:10.1038/s41420-025-02407-x · Cited by 101
Controllable C–H Alkylation of Polyethers via Iron Photocatalysis
Zhang, Z., Li, X., Zhou, D., Ding, S., Wang, M., & Zeng, R. (2023) · Journal of the American Chemical Society · doi:10.1021/jacs.3c01100 · Cited by 98
Dynamic GelMA/DNA Dual‐Network Hydrogels Promote Woven Bone Organoid Formation and Enhance Bone Regeneration
Zhu, M., Zhang, H., Zhou, Q., Sheng, S., Gao, Q., Geng, Z., Chen, X., Lai, Y., Jing, Y., Xu, K., et al. (2025) · Advanced Materials · doi:10.1002/adma.202501254 · Cited by 103
Supramolecular assemblies based on natural small molecules: Union would be effective
Hou, Y. J., Zou, L., Li, Q., Chen, M., Ruan, H., Sun, Z., Xu, X., Yang, J., & Ma, G. (2022) · Materials Today Bio · doi:10.1016/j.mtbio.2022.100327 · Cited by 97
Bioprinting microporous functional living materials from protein-based core-shell microgels
Ou, Y., Cao, S., Zhang, Y., Zhu, H., Guo, C., Yan, W., Xin, F., Dong, W., Zhang, Y., Narita, M., et al. (2023) · Nature Communications · doi:10.1038/s41467-022-35140-5 · Cited by 96
Programmable DNA Hydrogels as Artificial Extracellular Matrix
Wei, Y., Wang, K., Luo, S., Li, F., Zuo, X., Fan, C., & Li, Q. (2022) · Small · doi:10.1002/smll.202107640 · Cited by 97
Soft, Dynamic Hydrogel Confinement Improves Kidney Organoid Lumen Morphology and Reduces Epithelial–Mesenchymal Transition in Culture
Ruiter, F. A. A., Morgan, F. L. C., Roumans, N. J. T., Schumacher, A., Slaats, G. G. G., Moroni, L., LaPointe, V. L. S., & Baker, M. B. (2022) · Advanced Science · doi:10.1002/advs.202200543 · Cited by 96
Mucus-Inspired Dynamic Hydrogels: Synthesis and Future Perspectives
Bej, R., & Haag, R. (2022) · Journal of the American Chemical Society · doi:10.1021/jacs.1c13547 · Cited by 96
In situ modulation of intestinal organoid epithelial curvature through photoinduced viscoelasticity directs crypt morphogenesis
Yavitt, F. M., Kirkpatrick, B. E., Blatchley, M. R., Speckl, K. F., Mohagheghian, E., Moldovan, R., Wang, N., Dempsey, P. J., & Anseth, K. S. (2023) · Science Advances · doi:10.1126/sciadv.add5668 · Cited by 96
Programming hydrogels to probe spatiotemporal cell biology
Qazi, T. H., Blatchley, M. R., Davidson, M. D., Yavitt, F. M., Cooke, M. E., Anseth, K. S., & Burdick, J. A. (2022) · Cell stem cell · doi:10.1016/j.stem.2022.03.013 · Cited by 97
Advancing Synthetic Hydrogels through Nature‐Inspired Materials Chemistry
Soliman, B. G., Nguyen, A. K., Gooding, J. J., & Kilian, K. A. (2024) · Advanced Materials · doi:10.1002/adma.202404235 · Cited by 100
Biocompatible Conductive Hydrogels: Applications in the Field of Biomedicine
Yang, H., Lin, Z., Yang, Y. K., Jiang, T., Shang, J., & Luo, Z. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23094578 · Cited by 96
Hallmarks of stemness in mammalian tissues
Beumer, J., & Clevers, H. C. (2024) · Cell stem cell · doi:10.1016/j.stem.2023.12.006 · Cited by 92
Middle-out methods for spatiotemporal tissue engineering of organoids
Blatchley, M. R., & Anseth, K. S. (2023) · Nature Reviews Bioengineering · doi:10.1038/s44222-023-00039-3 · Cited by 85
3D Hydrogel Encapsulation Regulates Nephrogenesis in Kidney Organoids
Nerger, B. A., Sinha, S., Lee, N. N., Cheriyan, M., Bertsch, P., Johnson, C. P., Mahadevan, L., Bonventre, J. V., & Mooney, D. J. (2024) · Advanced Materials · doi:10.1002/adma.202308325 · Cited by 84
Extracellular matrix mimicking dynamic interpenetrating network hydrogel for skin tissue engineering
Wang, W., Dai, J., Huang, Y., Li, X., Yang, J., Zheng, Y., & Shi, X. (2023) · Chemical Engineering Journal · doi:10.1016/j.cej.2023.141362 · Cited by 84
Targeted mechanical stimulation via magnetic nanoparticles guides in vitro tissue development
Fattah, A. R. A., Kolaitis, N., Daele, K. V., Daza, B., Rustandi, G., & Ranga, A. (2023) · Nature Communications · doi:10.1038/s41467-023-41037-8 · Cited by 82
Strong, tough, fatigue-resistant and 3D-printable hydrogel composites reinforced by aramid nanofibers
Xing, H., He, X., Wang, Y., Zhang, X., Li, L., Wang, Y., Cheng, Z., Wu, H., Ge, Q., & Li, X. (2023) · Materials Today · doi:10.1016/j.mattod.2023.07.020 · Cited by 74
Bead-jet printing enabled sparse mesenchymal stem cell patterning augments skeletal muscle and hair follicle regeneration
Cao, Y., Tan, J., Zhao, H., Deng, T., Yunxia, H., Zeng, J., Li, J., Cheng, Y., Tang, J., Hu, Z., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-35183-8 · Cited by 75
Organic–inorganic composite hydrogels: compositions, properties, and applications in regenerative medicine
Wang, X., Wei, W., Guo, Z., Liu, X., Liu, J., Bing, T., Yu, Y., Yang, X., & Cai, Q. (2024) · Biomaterials Science · doi:10.1039/d3bm01766d · Cited by 72
Varying mechanical forces drive sensory epithelium formation
Xia, M., Wu, M., Li, Y., Liu, Y., Jia, G., Lou, Y., Ma, J., Gao, Q., Xie, M., Chen, Y., et al. (2023) · Science Advances · doi:10.1126/sciadv.adf2664 · Cited by 66
Tuning porosity of macroporous hydrogels enables rapid rates of stress relaxation and promotes cell expansion and migration
Nerger, B. A., Kashyap, K., Deveney, B. T., Lou, J., Hanan, B. F., Liu, Q., Khalil, A. S., Lungjangwa, T., Cheriyan, M., Gupta, A., et al. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2410806121 · Cited by 67
Marine‐Derived Hydrogels for Biomedical Applications
Lin, X., Wang, J., Wu, X., Luo, Y., Wang, Y., & Zhao, Y. (2022) · Advanced Functional Materials · doi:10.1002/adfm.202211323 · Cited by 64
Dynamic hydrogels for biofabrication: A review
Xu, R., Ooi, H. S., Bian, L., Ouyang, L., & Sun, W. (2025) · Biomaterials · doi:10.1016/j.biomaterials.2025.123266 · Cited by 66
The role of extracellular matrix viscoelasticity in development and disease
Courbot, O., & Elósegui-Artola, A. (2025) · npj Biological Physics and Mechanics. · doi:10.1038/s44341-025-00014-6 · Cited by 77
Lose the Stress: Viscoelastic Materials for Cell Engineering
Carvalho, E. M., & Kumar, S. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2022.03.058 · Cited by 61
Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids
Moura, B. S., Monteiro, M. V., Ferreira, L. P., Lavrador, P., Gaspar, V. M., & Mano, J. F. (2022) · Advanced Functional Materials · doi:10.1002/adfm.202202825 · Cited by 64
In vitro models to study human gut-microbiota interactions: Applications, advances, and limitations
Qi, Y., Yu, L., Tian, F., Zhao, J., & Zhai, Q. (2023) · Microbiological Research · doi:10.1016/j.micres.2023.127336 · Cited by 65
Morphodynamics of human early brain organoid development
Jain, A., Gut, G., Sanchis-Calleja, F., Tschannen, R., He, Z., Luginbühl, N., Zenk, F., Chrisnandy, A., Streib, S., Harmel, C., et al. (2025) · Nature · doi:10.1038/s41586-025-09151-3 · Cited by 73
Self-citation
Embryo‐uterine interaction coordinates mouse embryogenesis during implantation
Bondarenko, V., Nikolaev, M., Kromm, D., Belousov, R., Wolny, A., Blotenburg, M., Zeller, P. J., Rezakhani, S., Hugger, J., Uhlmann, V., et al. (2023) · The EMBO Journal · doi:10.15252/embj.2022113280 · Cited by 60
Self-citation
Stimuli-Responsive Polymers at the Interface with Biology
Boase, N. R. B., Gillies, E. R., Goh, R. Z. R., Kieltyka, R. E., Matson, J. B., Meng, F., Sanyal, A., & Sedláček, O. (2024) · Biomacromolecules · doi:10.1021/acs.biomac.4c00690 · Cited by 57
Hierarchical assembly of tryptophan zipper peptides into stress-relaxing bioactive hydrogels
Nguyen, A. K., Molley, T. G., Kardia, E., Ganda, S., Chakraborty, S., Wong, S. L., Ruan, J., Yee, B. E., Mata, J. P., Vijayan, A., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-41907-1 · Cited by 54
Integrating 3D Bioprinting and Organoids to Better Recapitulate the Complexity of Cellular Microenvironments for Tissue Engineering
Hu, Y., Zhu, T., Cui, H., & Cui, H. (2024) · Advanced Healthcare Materials · doi:10.1002/adhm.202403762 · Cited by 53
In situ covalent reinforcement of a benzene-1,3,5-tricarboxamide supramolecular polymer enables biomimetic, tough, and fibrous hydrogels and bioinks
Hafeez, S., Decarli, M. C., Aldana, A., Ebrahimi, M., Ruiter, F. A. A., Duimel, H., van Blitterswijk, C., Pitet, L. M., Moroni, L., & Baker, M. B. (2023) · Advanced Materials · doi:10.1002/adma.202301242 · Cited by 48
Emerging granular hydrogel bioinks to improve biological function in bioprinted constructs
Tuftee, C., Alsberg, E., Ozbolat, I. T., & Rizwan, M. (2023) · Trends in biotechnology · doi:10.1016/j.tibtech.2023.09.007 · Cited by 52
Hydrogels as promising platforms for engineered living bacteria-mediated therapeutic systems
Yu, S., Sun, H., Li, Y., Wei, S., Xu, J., & Liu, J. (2022) · Materials Today Bio · doi:10.1016/j.mtbio.2022.100435 · Cited by 47
Engineered biomimetic hydrogels for organoids
Li, Y., Saiding, Q., Wang, Z., & Cui, W. (2023) · Progress in Materials Science · doi:10.1016/j.pmatsci.2023.101216 · Cited by 46
Therapeutic supramolecular polymers: Designs and applications
Wang, H., Mills, J., Sun, B., & Cui, H. (2023) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2023.101769 · Cited by 45
Integration of 3D-printed cerebral cortical tissue into an ex vivo lesioned brain slice
Jin, Y., Mikhailova, E., Lei, M., Cowley, S. A., Sun, T., Yang, X., Zhang, Y., Liu, K., da Silva, D. C., Soares, L. C., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-41356-w · Cited by 48
Intrinsically cryopreservable, bacteriostatic, durable glycerohydrogel inks for 3D bioprinting
Liu, M., Jiang, S., Witman, N., Wang, H., Wang, W., Fu, W., & You, Z. (2023) · Matter · doi:10.1016/j.matt.2022.12.013 · Cited by 41
Innovations and Applications of Composite Hydrogels: from Polymer‐Based Systems to Metal‐Ion‐Doped and Functional Nanomaterial‐Enhanced Architectures
Zou, L., Li, Y., Feng, S., Wang, Z., Xiao, H., Chen, S., Wang, Y., He, L., & Mao, X. (2025) · Small · doi:10.1002/smll.202503147 · Cited by 43
Viscoelastic Notch Signaling Hydrogel Induces Liver Bile Duct Organoid Growth and Morphogenesis
Rizwan, M., Ling, C., Guo, C., Liu, T. W. B., Jiang, J., Bear, C. E., Ogawa, S., & Shoichet, M. S. (2022) · Advanced Healthcare Materials · doi:10.1002/adhm.202200880 · Cited by 41
Fiber Density and Matrix Stiffness Modulate Distinct Cell Migration Modes in a 3D Stroma Mimetic Composite HydrogelPreprint
Hiraki, H. L., Matera, D. L., Wang, W. Y., Prabhu, E. S., Zhang, Z., Midekssa, F. S., Argento, A. E., Buschhaus, J. M., Humphries, B. A., Luker, G. D., et al. (2021) · SSRN Electronic Journal · doi:10.1016/j.actbio.2022.09.043 · Cited by 40
Mucus‐Inspired Self‐Healing Hydrogels: A Protective Barrier for Cells against Viral Infection
Bej, R., Stevens, C. A., Nie, C., Ludwig, K., Degen, G. D., Kerkhoff, Y., Pigaleva, M. A., Adler, J. M., Bustos, N. A., Page, T. M., et al. (2024) · Advanced Materials · doi:10.1002/adma.202401745 · Cited by 39
An interdisciplinary framework for the characterization of extracellular matrix-hydrogels for biomedical applications
Martín‐Saldaña, S., Waeel, M. A., Alsharabasy, A. M., Daly, A. C., & Pandit, A. S. (2022) · Matter · doi:10.1016/j.matt.2022.09.020 · Cited by 43
Engineered Synthetic Matrices for Human Intestinal Organoid Culture and Therapeutic Delivery
Mulero‐Russe, A., & García, A. J. (2023) · Advanced Materials · doi:10.1002/adma.202307678 · Cited by 42
Mechanobiomaterials: Harnessing mechanobiology principles for tissue repair and regeneration
Lin, X. Y., Yang, H. S., Xia, Y., Wu, K., Chu, F., Zhou, H., Gao, H., & Yang, L. (2024) · Mechanobiology in Medicine · doi:10.1016/j.mbm.2024.100079 · Cited by 41
Mimicking the extracellular world: from natural to fully synthetic matrices utilizing supramolecular biomaterials
Rijns, L., Rutten, M. G. T. A., Vrehen, A. F., Aldana, A. A., Baker, M. B., & Dankers, P. Y. W. (2024) · Nanoscale · doi:10.1039/d4nr02088j · Cited by 37
Hydrogels mimicking the viscoelasticity of extracellular matrix for regenerative medicine: Design, application, and molecular mechanism
Li, L., Wang, S., Chen, Y., Dong, S., Zhang, C., Liao, L., & Zhang, W. (2024) · Chemical Engineering Journal · doi:10.1016/j.cej.2024.155206 · Cited by 40
Programmable DNA Hydrogel Provides Suitable Microenvironment for Enhancing TSPCS Therapy in Healing of Tendinopathy
Ge, Z., Li, W., Zhao, R., Xiong, W., Wang, D., Tang, Y., Fang, Q., Deng, X., Zhang, Z., Zhou, Y., et al. (2023) · Small · doi:10.1002/smll.202207231 · Cited by 35
Brush-Modified Hydrogels: Preparations, Properties, and Applications
Wu, B., Feng, E., Liao, Y., Liu, H., Tang, R., & Tan, Y. (2022) · Chemistry of Materials · doi:10.1021/acs.chemmater.2c01666 · Cited by 35
Tunable Mesoscopic Collagen Island Architectures Modulate Stem Cell Behavior
Nguyen, R. Y., Cabral, A. T., Rossello‐Martinez, A., Zulli, A., Gong, X., Zhang, Q., Yan, J. Q., & Mak, M. (2023) · Advanced Materials · doi:10.1002/adma.202207882 · Cited by 36
Physical strategies to engineer supramolecular composite hydrogels for advanced biomedical applications
Baddi, S., Dang-i, A. Y., Gao, F., Qiu, X., & Feng, C. (2025) · Progress in Materials Science · doi:10.1016/j.pmatsci.2025.101428 · Cited by 35
Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review
Li, Z., Yue, M., Liu, Y., Zhang, P., Qing, J., Liu, H., & Zhou, Y. (2022) · Gels · doi:10.3390/gels8060379 · Cited by 37
Double-Network Hydrogel with Strengthened Mechanical Property for Controllable Release of Antibacterial Peptide
Tan, T., Hou, Y., Zhang, Y., & Wang, B. (2024) · Biomacromolecules · doi:10.1021/acs.biomac.3c01290 · Cited by 33
Advances in biomimetic hydrogels for organoid culture
Luo, L., Liu, L., Ding, Y., Dong, Y., & Ma, M. (2023) · Chemical Communications · doi:10.1039/d3cc01274c · Cited by 36
Importance of Molecular and Bulk Dynamics in Supramolecular Hydrogels in Dictating Cellular Spreading
Rijns, L., Peeters, J. W., Hendrikse, S. I. S., Vleugels, M. E. J., Lou, X., Janssen, H. M., Meijer, E. W., & Dankers, P. Y. W. (2023) · Chemistry of Materials · doi:10.1021/acs.chemmater.3c01676 · Cited by 34
Revisiting the biophysical aspects of extracellular-matrix-mimicking hydrogels: what cells see vs. what cells feel
Sui, J., Pragnère, S., & Kurniawan, N. A. (2025) · Biomaterials Science · doi:10.1039/d5bm00210a · Cited by 35
Dynamic hydrogel mechanics in organoid engineering: From matrix design to translational paradigms
Zhang, C., Shen, Y. C., Huang, M.-Y., Wang, G., Miao, Q., Shi, H., Gao, R., Wang, K., & Luo, M. B. (2025) · Bioactive Materials · doi:10.1016/j.bioactmat.2025.09.021 · Cited by 43
A multicellular self-organized probiotic platform for oral delivery enhances intestinal colonization
Liu, H., Chen, Z., Lin, Q., Chen, Y., Hong, L., Zhong, J., Cai, Z., Wang, Z., & Cui, W. (2025) · Nature Communications · doi:10.1038/s41467-025-62349-x · Cited by 32
3D printed arrowroot starch-gellan scaffolds for wound healing applications
Joseph, A., Fathah Muhammad, L., Vijayan, A. S., Xavier, J., Megha, K. B., Karthikeyan, A., Gopinath, N., Mohanan, P. V., & Nair, B. G. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.130604 · Cited by 28
Mechanobiology of 3D cell confinement and extracellular crowding
André, G. D. S., & Labouesse, C. (2024) · Biophysical Reviews · doi:10.1007/s12551-024-01244-z · Cited by 30
A bioactive calcium silicate nanowire-containing hydrogel for organoid formation and functionalization
Ma, W., Zheng, Y., Yang, G.-Z., Zhang, H., Lu, M., Ma, H., Wu, C., & Lu, H. (2024) · Materials Horizons · doi:10.1039/d4mh00228h · Cited by 27
Lamination-based organoid spatially resolved transcriptomics technique for primary lung and liver organoid characterization
Ma, S., Wang, W., Zhou, J., Liao, S., Hai, C., Hou, Y., Zhou, Z., Wang, Z., Su, Y., Zhu, Y., et al. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2408939121 · Cited by 29
Optimizing phenol-modified hyaluronic acid for designing shape-maintaining biofabricated hydrogel scaffolds in soft tissue engineering
Simińska‐Stanny, J., Hachemi, F., Dodi, G., Cojocaru, F.-D., Gardikiotis, I., Podstawczyk, D. A., Delporte, C., Jiang, G., Nie, L., & Shavandi, A. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.125201 · Cited by 27
Spatial and Temporal Control of 3D Hydrogel Viscoelasticity through Phototuning
Crandell, P., & Stowers, R. S. (2023) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.3c01099 · Cited by 28
The pivotal application of patient-derived organoid biobanks for personalized treatment of gastrointestinal cancers
Yu, Y., Zhu, Y.-J., Xiao, Z., Chen, Y., Chang, X., Liu, Y., Tang, Q., & Zhang, H. (2022) · Biomarker Research · doi:10.1186/s40364-022-00421-0 · Cited by 27
Listen to Your Gut: Key Concepts for Bioengineering Advanced Models of the Intestine
Cameron, O., Neves, J. F., & Gentleman, E. (2023) · Advanced Science · doi:10.1002/advs.202302165 · Cited by 26
3D Printing of Maturable Tissue Constructs Using a Cell‐Adaptable Nanocolloidal Hydrogel
Li, L., Liu, H., Zhao, Y., Liu, X., Dong, Y., Luo, J., Jiang, X., Zhang, Y., Zhu, Q., Yuan, X., et al. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202402341 · Cited by 24
Exploiting Matrix Stiffness to Overcome Drug Resistance
Aydın, H. B., Özçelikkale, A., & Acar, A. (2024) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.4c00445 · Cited by 23
Enabling 3D printability and vascular morphogenesis with double network dynamic hydrogels
Xu, R., Dou, B., Yu, S., Wang, Z., Zhang, Y., Leng, L., Ouyang, L., & Sun, W. (2025) · Materials Today · doi:10.1016/j.mattod.2025.01.019 · Cited by 25
User‐Controlled 4D Biomaterial Degradation with Substrate‐Selective Sortase Transpeptidases for Single‐Cell Biology
Bretherton, R. C., Haack, A. J., Kopyeva, I., Rahman, F., Kern, J. D., Bugg, D., Theberge, A. B., Davis, J., & DeForest, C. A. (2023) · Advanced Materials · doi:10.1002/adma.202209904 · Cited by 23
Reconfigurable scaffolds for adaptive tissue regeneration
Peng, M., Zhao, Q., Wang, M., & Du, X. (2023) · Nanoscale · doi:10.1039/d3nr00281k · Cited by 24
Biology-driven material design for ischaemic stroke repair
Phan, N. V., Rathbun, E. M., Ouyang, Y., Carmichael, S. T., & Segura, T. (2023) · Nature Reviews Bioengineering · doi:10.1038/s44222-023-00117-6 · Cited by 26
The biological macromolecules constructed Matrigel for cultured organoids in biomedical and tissue engineering
Zhao, K.-Y., Du, Y., Cao, H., Su, L., Su, X., & Li, X. (2024) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2024.114435 · Cited by 25
Early spinal cord development: from neural tube formation to neurogenesis
Saade, M., & Martı́, E. (2025) · Nature reviews. Neuroscience · doi:10.1038/s41583-025-00906-5 · Cited by 26
Dynamic Hydrogels with Tunable Mechanics for 3D Organoid Derivation
Xie, X., Chen, X., Zhou, J. X., Wang, T., Yang, G., Han, F., & Zhao, W. (2025) · Small · doi:10.1002/smll.202501862 · Cited by 23
Fully Synthetic Hydrogels Promote Robust Crypt Formation in Intestinal Organoids
Hushka, E. A., Blatchley, M. R., Macdougall, L. J., Yavitt, F. M., Kirkpatrick, B. E., Bera, K., Dempsey, P. J., & Anseth, K. S. (2025) · Advanced Materials · doi:10.1002/adma.202509672 · Cited by 25
Translational organoid technology – the convergence of chemical, mechanical, and computational biology
Zhang, W., Li, J., Zhou, J., Rastogi, A., & Ma, S. (2022) · Trends in biotechnology · doi:10.1016/j.tibtech.2022.03.003 · Cited by 23
Electrically Powered Dissipative Hydrogel Networks Reveal Transient Stiffness Properties for Out-of-Equilibrium Operations
Baretta, R., & Frasconi, M. (2024) · Journal of the American Chemical Society · doi:10.1021/jacs.3c12653 · Cited by 23
Bridging the organoid translational gap: integrating standardization and micropatterning for drug screening in clinical and pharmaceutical medicine
Yang, H., Li, J., Wang, Z., Khutsishvili, D., Tang, J., Zhu, Y., Cai, Y., Dai, X., & Ma, S. (2024) · Life Medicine · doi:10.1093/lifemedi/lnae016 · Cited by 25
Dynamic duo: Cell–extracellular matrix interactions in hair follicle development and regeneration
Fujiwara, H. (2024) · Developmental Biology · doi:10.1016/j.ydbio.2024.07.012 · Cited by 22
Material Engineering in Gut Microbiome and Human Health
Yang, L., Hung, L. Y., Zhu, Y., Ding, S., Margolis, K. G., & Leong, K. W. (2022) · Research · doi:10.34133/2022/9804014 · Cited by 24
Biomaterials to promote vascularization in tissue engineering organs and ischemic fibrotic diseases
Zhao, W., Zhu, J., Hang, J., & Zeng, W. (2022) · MedComm – Biomaterials and Applications · doi:10.1002/mba2.16 · Cited by 20
Integrin-activating Yersinia protein Invasin sustains long-term expansion of primary epithelial cells as 2D organoid sheets
Wijnakker, J. J. A. P. M., van Son, G. J. F., Krueger, D., van de Wetering, W. J., López‐Iglesias, C., Schreurs, R., van Rijt, F. L. H., Lim, S., Lin, L., Peters, P. J., et al. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2420595121 · Cited by 20
3D bioprinting bone/cartilage organoids: construction, applications, and challenges
Gao, D., Li, R., Pan, J., Li, C., Zhang, W., Qin, L., & Lai, Y. (2025) · Journal of Orthopaedic Translation · doi:10.1016/j.jot.2025.08.008 · Cited by 22
Highly Stretchable, Swelling-Resistant, Self-Healed, and Biocompatible Dual-Reinforced Double Polymer Network Hydrogels
Fan, W., Jensen, L. R., Dong, Y., Deloria, A. J., Xing, B., Yu, D., & Smedskjær, M. M. (2022) · ACS Applied Bio Materials · doi:10.1021/acsabm.2c00856 · Cited by 20
Hydrogels with brain tissue-like mechanical properties in complex environments
Wang, J., Zhang, Y., Lei, Z., Wang, J., Zhao, Y., Sun, T., Jiang, Z., Zhou, L., Liu, Z., Liu, Y., et al. (2023) · Materials & Design · doi:10.1016/j.matdes.2023.112338 · Cited by 20
3D extrusion bioprinting: rational bioink design and advanced fabrication techniques
Jergitsch, M., & Mateos‐Timoneda, M. A. (2025) · Trends in biotechnology · doi:10.1016/j.tibtech.2025.06.008 · Cited by 19

Authors: Chrisnandy, A., Blondel, D., Rezakhani, S., Broguiere, N., & Lutolf, M. P.
Affiliations: École Polytechnique Fédérale de Lausanne (CH)
ORCID: Chrisnandy, Antonius
Rezakhani, Saba
Broguiere, Nicolas
Lutolf, Matthias P.
Venue: Nature Materials
Publisher: Springer Science and Business Media LLC
Published: 2021-11-15
Volume: 21
Issue: 4
Pages: 479-487
ISSN: 1476-1122 (ISSN-L)
1476-4660
DOI: 10.1038/s41563-021-01136-7
OpenAlex: W3211744428
Cited by: 237
References: 53
Citations by year: 2026: 32 · 2025: 59 · 2024: 54 · 2023: 55 · 2022: 35 · 2021: 2
FWCI: 11.52
Type: journal article
Language: en
Open access: green
infoscience.epfl.ch
Licence: cc-by-nc-nd
www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: Nature Materials (publishedVersion)
PubMed (publishedVersion)
Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
Topics: Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords: synthetic hydrogels, dynamic hydrogels, stress relaxation, intestinal organoids, symmetry breaking, Paneth cells, YAP, extracellular matrix, tissue expansion
MeSH terms: Animals, Extracellular Matrix, Organoids, Stem Cells, Hydrogels, Organogenesis, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Synthetic dynamic hydrogels promote degradation-independent in vitro organogenesis

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks