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A malaria vaccine adjuvant based on recombinant antigen binding to liposomes
Open accessAbstract
Pfs25 is a malaria transmission-blocking vaccine antigen candidate, but its apparently limited immunogenicity in humans has hindered clinical development. Here, we show that recombinant, polyhistidine-tagged (his-tagged) Pfs25 can be mixed at the time of immunization with pre-formed liposomes containing cobalt porphyrin-phospholipid, resulting in spontaneous nanoliposome antigen particleization (SNAP). Antigens are stably presented in uniformly orientated display via his-tag insertion in the cobalt porphyrin-phospholipid bilayer, without covalent modification or disruption of antigen conformation. SNAP immunization of mice and rabbits is well tolerated with minimal local reactogenicity, and results in orders-of-magnitude higher functional antibody generation compared with other 'mix-and-inject' adjuvants. Serum-stable antigen binding during transit to draining lymph nodes leads to enhanced antigen uptake by phagocytic antigen-presenting cells, with subsequent generation of long-lived, antigen-specific plasma cells. Seamless multiplexing with four additional his-tagged Plasmodium falciparum polypeptides induces strong and balanced antibody production, illustrating the simplicity of developing multistage particulate vaccines with SNAP immunization.
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“a recent study used spontaneous nanoliposome antigen particleization to show efficacy of a multistage, multi-antigen vaccine in mice and rabbits 116 .”
From Nanotechnology approaches for global infectious diseases (2021) · cites this paper as [116]
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Metadata
- Authors
- Huang, W.-C., Deng, B., Lin, C., Carter, K. A., Geng, J., Razi, A., He, X., Chitgupi, U., Federizon, J., Sun, B., Long, C. A., Ortega, J., Dutta, S., King, C. R., Miura, K., Lee, S.-M., & Lovell, J. F.
- Affiliations
- University at Buffalo, State University of New York (US)
- National Institute of Allergy and Infectious Diseases (US)
- McGill University (CA)
- Walter Reed Army Institute of Research (US)
- Corresponding author
- Lovell, Jonathan F.
- ORCID
- Venue
- Nature Nanotechnology
- Published
- 2018-10-08
- Volume
- 13
- Issue
- 12
- Pages
- 1174-1181
- ISSN
- 1748-3387 (ISSN-L)
- 1748-3395
- PMC
- PMC6286227
- OpenAlex
- W2894881231
- Cited by
- 148
- References
- 51
- Citations by year
- 2026: 10 · 2025: 18 · 2024: 20 · 2023: 19 · 2022: 20 · 2021: 19 · 2020: 23 · 2019: 17 · 2018: 2
- FWCI
- 14.52
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Nanotechnology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (DP5OD017898)
- National Institute of Allergy and Infectious Diseases (R21AI122964)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- malaria vaccine, vaccine adjuvants, Pfs25, transmission-blocking vaccine, Plasmodium falciparum, antigen-presenting cells, plasma cells, functional antibodies, particulate vaccines
- MeSH terms
- RAW 264.7 Cells, Adjuvants, Immunologic, Animals, Antibody Formation, Antigens, Protozoan, Female, Humans, Immunization, Liposomes, Plasmodium falciparum, Rabbits, Recombinant Proteins, Protozoan Proteins, Malaria, Falciparum, Malaria Vaccines, Mice
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