The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Individual brain organoids reproducibly form cell diversity of the human cerebral cortex
Open accessAbstract
Experimental models of the human brain are needed for basic understanding of its development and disease 1. Human brain organoids hold unprecedented promise for this purpose; however, they are plagued by high organoid-to-organoid variability 2,3. This has raised doubts as to whether developmental processes of the human brain can occur outside the context of embryogenesis with a degree of reproducibility that is comparable to the endogenous tissue. Here we show that an organoid model of the dorsal forebrain can reliably generate a rich diversity of cell types appropriate for the human cerebral cortex. We performed single-cell RNA-sequencing analysis of 166,242 cells isolated from 21 individual organoids, finding that 95% of the organoids generate a virtually indistinguishable compendium of cell types, following similar developmental trajectories and with a degree of organoid-to-organoid variability comparable to that of individual endogenous brains. Furthermore, organoids derived from different stem cell lines show consistent reproducibility in the cell types produced. The data demonstrate that reproducible development of the complex cellular diversity of the central nervous system does not require the context of the embryo, and that establishment of terminal cell identity is a highly constrained process that can emerge from diverse stem cell origins and growth environments.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“Furthermore, several studies have shown that guided cortical organoid differentiation yields highly reproducible organoids as compared to unguided organoid differentiation (72–74).”
From Understanding monocyte-driven neuroinflammation in Alzheimer’s disease using human cortical organoid microphysiological systems (2025) · cites this paper as [74]
The cited source says what the claim attributes to it.
In the citing paper
“Organoid development followed the expected temporal changes (Lancaster et al., 2013; Velasco et al., 2019; Watanabe et al., 2017; Yoon et al., 2019; Figures 1B–1D; Figure S1A).”
From ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids (2021) · cites this paper as Velasco et al., 2019
The cited source says what the claim attributes to it.
In the citing paper
“scRNA-seq analysis of human intestinal and brain organoids used random forest classifiers to identify the cell types in their organoid cultures;73,74”
From Single-cell transcriptomics of human-skin-equivalent organoids (2023) · cites this paper as [74]
The check wasn’t confident either way, so it gives no verdict — never disputed.
In the citing paper
“All cell sub-types were present in WA25 and DSP-GFP organoids, but in different proportions (Fig. 2b, c), suggesting further protocol optimization may be needed to reduce variability19.”
From Hair-bearing human skin generated entirely from pluripotent stem cells (2020) · cites this paper as [19]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Classification and Regression by randomForest
Liaw, A. I., & Wiener, M. C. (2007) · Cited by 18,594
Integrating single-cell transcriptomic data across different conditions, technologies, and species
Butler, A., Hoffman, P., Smibert, P., Papalexi, E., & Satija, R. (2018) · Nature Biotechnology · doi:10.1038/nbt.4096 · Cited by 13,174
Massively parallel digital transcriptional profiling of single cells
Zheng, G. X. Y., Terry, J. M., Belgrader, P., Ryvkin, P., Bent, Z. W., Wilson, R., Ziraldo, S. B., Wheeler, T. D., McDermott, G. P., Zhu, J., et al. (2017) · Nature Communications · doi:10.1038/ncomms14049 · Cited by 7,353
Cerebral organoids model human brain development and microcephaly
Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372
Reversed graph embedding resolves complex single-cell trajectories
Qiu, X., Mao, Q., Tang, Y., Wang, L., Chawla, R., Pliner, H. A., & Trapnell, C. (2017) · Nature Methods · doi:10.1038/nmeth.4402 · Cited by 5,070
Scrublet: computational identification of cell doublets in single-cell transcriptomic data
Wolock, S. L., Lopez, R., & Klein, A. M. (2018) · doi:10.1101/357368 · Cited by 2,758 · cited 1×
“1 39 was used to assess the effect of droplets that may have contained more than one cell, with an expected doublet rate of 0.1 and a score threshold of 0.39, chosen based on histograms of simulated doublet scores (Extended Data Fig.”
InfluentialScrublet: computational identification of cell doublets in single-cell transcriptomic data
Wolock, S. L., Lopez, R., & Klein, A. M. (2019) · Cell Systems · doi:10.1016/j.cels.2018.11.005 · Cited by 2,758
Zhang, Y., Sloan, S. A., Clarke, L. E., Caneda, C., Plaza, C. A., Blumenthal, P. D., Vogel, H., Steinberg, G. K., Edwards, M. S. B., Li, G., et al. (2016) · Neuron · doi:10.1016/j.neuron.2015.11.013 · Cited by 2,295
Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure
Qian, X., Nguyen, H. N., Song, M. M., Hadiono, C., Ogden, S. C., Hammack, C., Yao, B., Hamersky, G. R., Jacob, F., Zhong, C., et al. (2016) · Cell · doi:10.1016/j.cell.2016.04.032 · Cited by 2,127
Molecular diversity and specializations among the cells of the adult mouse brain
Saunders, A., Macosko, E. Z., Wysoker, A., Goldman, M., Krienen, F. M., de Rivera, H., Bien, E., Baum, M., Bortolin, L., Wang, S., et al. (2018) · Cell · doi:10.1016/j.cell.2018.07.028 · Cited by 1,531
Cited by
Sorted by citations
Items 801–900 of 999
Human adherent cortical organoids in a multi-well format
van der Kroeg, M., Bansal, S., Unkel, M., Smeenk, H., Kushner, S. A., & de Vrij, F. M. S. (2026) · eLife · doi:10.7554/elife.98340.3 · Cited by 2
Morphogenetic Designs, and Disease Models in Central Nervous System Organoids
Bock, M., Hong, S. J., Zhang, S., Yu, Y., Lee, S., Shin, H.-E., Choi, B. H., & Han, I. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25147750 · Cited by 3
Neuron-Astrocyte Interactions: A Human Perspective
Pio, T., Hill, E. J., Kebede, N., Andersen, J., & Sloan, S. A. (2024) · Advances in neurobiology · doi:10.1007/978-3-031-64839-7_4 · Cited by 2
Using cortical organoids to understand the pathogenesis of malformations of cortical development
Winden, K. D., Gisser, I., & Şahin, M. (2025) · Frontiers in Neuroscience · doi:10.3389/fnins.2024.1522652 · Cited by 2
Generation of 3D Midbrain Organoids from Human-Induced Pluripotent Stem Cells
Yangzom, T., Chen, A., & Liang, K. X. (2025) · Journal of Visualized Experiments · doi:10.3791/67228 · Cited by 2
Chun, M. Y., Jung, S., Choe, J., Lee, S., Kim, H., Son, H. J., Choi, Y., Cho, M., Kim, B., Jang, H., et al. (2025) · Alzheimer s & Dementia · doi:10.1002/alz.70660 · Cited by 2
Hydrodynamic Focusing-Enabled Blood Vessel Fabrication for in Vitro Modeling of Neural Surrogates
DiVito, K. A., Luo, J., Rogers, K. E., Sundaram, S., Roberts, S., Dahal, B., van Hoek, M. L., Kehn‐Hall, K., Delehanty, J. B., & Adams, A. A. (2021) · Journal of Medical and Biological Engineering · doi:10.1007/s40846-021-00629-9 · Cited by 3
Loss of PAX6 alters the excitatory/inhibitory neuronal ratio in human cerebral organoids
Chan, W. K., Abdulla, S., Zhao, L., Negro, D., Munro, V. M., Thomson, S. R., Marshall, H., Kozić, Z., Brown, M., Barbato, M., et al. (2026) · Development · doi:10.1101/2023.07.31.551262 · Cited by 2
A Geometric Milieu Inside the Brain
Tozzi, A., Yurkin, A., & Peters, J. F. (2021) · Foundations of Science · doi:10.1007/s10699-021-09798-x · Cited by 2
Sebastian, R., Jin, K., Pavon, N., Bansal, R., Potter, A., Song, Y., Babu, J., Gabriel, R., Sun, Y., Aronow, B. J., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.08.24.505165 · Cited by 2
Neuroimmune cortical organoids overexpressing C4A exhibit multiple schizophrenia endophenotypesPreprint
Stanton, M. M., Modan, S., Taylor, P. M., Hariani, H. N., Sorokin, J. M., Rash, B. G., Rao, S. B., Tobon, A., Enriquez, L., Dang, B., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.01.20.524955 · Cited by 2
Boreland, A. J., Stillitano, A. C., Lin, H.-C., Abbo, Y., Hart, R. P., Jiang, P., Pang, Z. P., & Rabson, A. B. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.10.25.563950 · Cited by 2
Loss of UBE3A impacts both neuronal and non-neuronal cells in human cerebral organoidsPreprint
Estridge, R. C., Yagci, Z. B., Sen, D., Ptacek, T., Simon, J. M., & Keung, A. J. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.02.09.579672 · Cited by 2
Narasipura, S. D., Zayas, J. P., Ash, M. K., Reyes, A. F., Shull, T. L., Gambut, S., Schneider, J. R., Lorenzo-Redondo, R., Al‐Harthi, L., & Mamede, J. I. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.06.13.598579 · Cited by 2
Kim, E., Kim, Y. H., Hong, S.-J., Kim, I. S., Lee, J. H., Yoo, J.-Y., Kim, J., Yoo, K., Kim, J., Choi, J., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.10.01.615952 · Cited by 2
Ditzer, N., Senoglu, E., Schütze, T. M., Nikolaidi, A., Kolodziejczyk, A., Sameith, K., Dietz, S., Derihaci, R. P., Birdir, C., Eugster, A., et al. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.5017114 · Cited by 2
Reconstitution of Human Brain Cell Diversity in Organoids via Four ProtocolsPreprint
Naas, J., Balmaña, M., Holcik, L., Novatchkova, M., Dobnikar, L., Krausgruber, T., Ladstätter, S., Bock, C., von Haeseler, A., Esk, C., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.11.15.623576 · Cited by 2
Repetitive stimulation modifies network characteristics of neural organoid circuitsPreprint
Chow, S. Y. A., Hu, H., Duenki, T., Asakura, T., Sugimura, S., & Ikeuchi, Y. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.01.16.633310 · Cited by 2
Ojeda, A. V., Awabdeh, D., Brewster, B., Rockne, R. C., O’Meally, D., Yin, H. H., Carlesso, N., Brown, C. E., Gutova, M., & Barish, M. E. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.02.12.637976 · Cited by 2
Defined human tri-lineage brain microtissuesPreprint
Uenaka, T., Jung, S., Kumar, I., Vodehnal, K., Rastogi, M., Yoo, Y., Koontz, M., Thome, C., Li, W., Chan, T. C., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.13016/m2y2ln-j1l8 · Cited by 2
Harding, A. T., Zhang, Y., Chen, J.-J., Antonucci, J. M., Richards, A. L., Leger, V., Whittaker, C. A., Vanrobaeys, Y. S., Agarwal, D., Lungjangwa, T., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.08.12.669648 · Cited by 2
Daheron, L. M., & Chen, I. P.-F. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-822277-5.00008-0 · Cited by 1
Menon, R., Petrucci, L., Lohrer, B., Zhang, J., Schulze, M., Schichor, C., Winner, B., Winkler, J., Riemenschneider, M. J., Kühn, R., et al. (2023) · Cellular Reprogramming · doi:10.1089/cell.2023.0008 · Cited by 1
Promising Prospects for Human Cerebral Organoids to Advance Alzheimer's Disease Research
López, V., Rosca, A., Liste, I., Mateos-Martínez, P., Coronel, R., González-Sastre, R., Martín-Benito, S., & López, V. (2023) · Progress in Neurobiology · doi:10.60124/j.pneuro.2023.20.05 · Cited by 1
Jaramillo, C. J., Gibbons, T. F., Rose, R., & Walsh, J. (2024) · American Journal of Forensic Medicine & Pathology · doi:10.1097/paf.0000000000000916 · Cited by 1
Gubinelli, F., Salazar, J. M., Kaspar, J., Ortiz, I. S., Schafer, S. T., & Burbulla, L. F. (2024) · Neuromethods · doi:10.1007/978-1-0716-4083-8_17 · Cited by 1
Organoid Computing: Leveraging Organoid Neural Networks for Artificial Intelligence
Li, K., Gu, L., Cai, H., & Guo, F. (2024) · Collaborative bioethics · doi:10.1007/978-3-031-72371-1_12 · Cited by 1
Bertacchi, M., Maharaux, G., & Studer, M. (2025) · BIO-PROTOCOL · doi:10.21769/bioprotoc.5354 · Cited by 1
Neuronal branching in stem cell models of mitochondrial and neurological diseases
Lickfett, S., Menacho, C., Cambridge, S. B., & Prigione, A. (2025) · Stem Cells · doi:10.1093/stmcls/sxaf050 · Cited by 2
Glucose Levels Impact the Morphology and Cell Type Composition of Human Cerebral Organoids
Kelkar, G. R., Rao, B. M., & Keung, A. J. (2026) · Organoids · doi:10.3390/organoids5020011 · Cited by 1
Brain organoids – Challenges while constructing organoids
Parthasarathy, S., Bupesh, G., & Konyak, L. M. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-29898-1.00006-x · Cited by 1
CRISPR-Based Functional Genomics in Pluripotent Stem Cells
Zahedi, S. (2025) · Stem Cell Reviews and Reports · doi:10.1007/s12015-025-11019-y · Cited by 1
Vascular dysfunctions during radiation retinopathy
Niaudet, C., Mathis, T., Pham, T.-N., Quintyn, J. C., Paris, F., & Thariat, J. (2025) · Radiotherapy and Oncology · doi:10.1016/j.radonc.2025.111294 · Cited by 1
P2X purinergic receptors are required for correct cortical development in human brain organoids
Benito-León, M., Serrano-López, J., Llorente-Sáez, C., Arribas-Blázquez, M., Olivos-Oré, L. A., Pravatà, V. M., Pérez‐Sen, R., Delicado, E. G., Drukker, M., Artalejo, A. R., et al. (2025) · Neuropharmacology · doi:10.1016/j.neuropharm.2025.110784 · Cited by 1
All-in-one generation and multiomic profiling of human whole brain organoid on a millifluidic plate
Zhao, W., Wang, Y., Chen, T., Shen, M., Wang, J., Huang, X., Zhu, L., Yu, T., Zhang, Z., Yang, Y., et al. (2025) · Materials Today Bio · doi:10.1016/j.mtbio.2025.102653 · Cited by 2
Jiang, Y., Guerra, D. B., Voshart, D. C., Hageman, E., Nazario, L. R., van Goethem, M., Coppes, R. P., & Barazzuol, L. (2026) · Stem Cell Reports · doi:10.1016/j.stemcr.2025.102777 · Cited by 1
Transcriptional code for circuit integration in the injured brain by transplanted human neurons
Wang, Z., Zheng, D., Chou, S.-M., Kang, P. J., Ye, F., & Zhang, S. (2026) · Cell stem cell · doi:10.1016/j.stem.2025.12.008 · Cited by 2
He, B., & Smedler, E. (2026) · Neuroscience Applied · doi:10.1016/j.nsa.2026.106982 · Cited by 1
Organizers in a dish: Modeling human CNS morphogenesis
Miller, G., Sanchez, D. J. L.-D., Martínez, J. G., Justin, A. W., Lancaster, M. A., & Guglielmi, L. (2026) · Developmental Cell · doi:10.1016/j.devcel.2026.01.003 · Cited by 2
Getting the message: low-plex detection of RNA in tissues
Spencer‐Dene, B., & Miranda, E. (2026) · Frontiers in RNA Research · doi:10.3389/frnar.2026.1749833 · Cited by 1
Developmental stage–dependent neurotoxicity of sevoflurane: evidence from brain organoids
Chen, N., Zhang, Y., Chen, Y., & Chen, G. (2026) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2026.1774547 · Cited by 1
Recent advances in nanomaterial-based brain organoid on-a-chip for drug evaluation
Shin, M., Lee, M.-J., Lee, S., Lee, K., & Choi, J.-W. (2026) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2026.118612 · Cited by 1
Chen, C., Gan, A., Huang, C., Weng, Y., & Wang, T. (2026) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2026.1792350 · Cited by 1
Mostafa, A., Khalil, S. M., Shaheraldin, A. A., Alahmadi, H. A., Mattar, Y. K., El-Bahrawy, N. R., Issa, I. K., El-fiki, R. A., Elazim, N. M. A., Attia, A. M., et al. (2026) · Stem cell biology and regenerative medicine · doi:10.1007/978-3-032-12705-1_4 · Cited by 1
Modeling pediatric low-grade glioma heterogeneity using human forebrain organoids
Leva, G., Santomaso, L., Gianesello, M., Patrizi, S. T., Ress, F., Cocchini, F., Antonacci, C., Gianno, F., Abballe, L., Lago, C., et al. (2026) · Molecular Cancer · doi:10.1186/s12943-026-02612-x · Cited by 1
Cell type-agnostic transcriptomic signatures enable uniform comparisons of neural maturation
Venkatesan, S., Werner, J. M., Li, Y., & Gillis, J. (2026) · PLoS Biology · doi:10.1371/journal.pbio.3003757 · Cited by 1
Zhang, W., Wang, T. W., Yao, M., Su, L., Xie, L., & Pan, P. (2026) · Open Research (University of Surrey) · doi:10.1002/viw.20250207 · Cited by 1
Ishan, M., JImenez-Macias, J. L., & Read, R. D. (2026) · Frontiers in Oncology · doi:10.3389/fonc.2026.1803705 · Cited by 1
Huang, Y., Wang, Z., Li, H., Wang, Z., Luo, G., Zhao, L., Zeng, P., Zhu, X., Xue, A., Bian, G., et al. (2026) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2026.142493 · Cited by 1
Namipashaki, A., Yu, H., Bellgrove, M. A., & Hawi, Z. (2026) · Cells · doi:10.3390/cells15100931 · Cited by 1
Raji, H., Bertoli, F., Maria Jose Perez, J., Lam, A., Volpicelli‐Daley, L. A., & Deleidi, M. (2026) · Molecular Psychiatry · doi:10.1038/s41380-026-03667-4 · Cited by 1
Anwar, S., & Yokota, T. (2026) · Genes · doi:10.3390/genes17070780 · Cited by 1
Chen, S., Zhang, W., Dai, L., Kuang, Y., Yang, H., Gu, J., Peng, K., Jiang, N., Liu, H., & Li, X. (2026) · Cancers · doi:10.3390/cancers18162601 · Cited by 1
Antunes, A. S. L. M., Athié, M. C. P., Silveira, A. C. C., Renner, J., Dias, E. V., dos Santos, J. V. R., Hrvoj-Mihić, B., Canateli, C., Meidânis, J., Muotri, A. R., et al. (2026) · Neuroscience · doi:10.1016/j.neuroscience.2026.08.028 · Cited by 2
Pio, T., Bettaiah, M., Zhao, R., Wariyar, S. S., Mouhi, S., Hill, E. J., Sloan, S. A., Ou, Z., Yu, A. J. R., Adams, C., et al. (2026) · Stem Cell Reports · doi:10.1016/j.stemcr.2026.103114 · Cited by 1
Benchmarking pluripotent stem cell-derived organoid models
Angeles, A. D. L., & Tunbridge, E. M. (2020) · Experimental Neurology · doi:10.1016/j.expneurol.2020.113333 · Cited by 1
Research progress of neuronal relay strategies in repairing spinal cord injuries
Lai, B., Ding, Y., Zeng, Y., Li, G., & Zeng, X. (2020) · Scientia Sinica Vitae · doi:10.1360/ssv-2020-0017 · Cited by 1
Organoids: the third dimension of human brain development and disease
Kouroupi, G., Prodromidou, K., Papastefanaki, F., Taoufik, E., & Matsas, R. (2021) · The International Journal of Developmental Biology · doi:10.1387/ijdb.210158gk · Cited by 1
Shaker, M. R., Hunter, Z. L., & Wolvetang, E. J. (2022) · Journal of Visualized Experiments · doi:10.3791/63714-v · Cited by 1
Farahani, N. I., Chan, J., Añonuevo, A., Chew, L. H., & Knock, E. (2023) · Neuromethods · doi:10.1007/978-1-0716-2875-1_21 · Cited by 1
Neural Induction and Regionalization
Wigle, J. T., & Eisenstat, D. D. (2023) · Springer eBooks · doi:10.1007/978-3-031-20792-1_2 · Cited by 1
Zooming into finer brain structures in vitro
Zhang, A., & Wang, Y. (2023) · Cell stem cell · doi:10.1016/j.stem.2023.04.009 · Cited by 1
Telencephalic organoids as model systems to study cortical development and diseases
Yang, W. S., Kiral, F. R., & Park, I. (2024) · Organoid · doi:10.51335/organoid.2024.4.e1 · Cited by 1
Stem cell modeling of nervous system tumors
Furnari, F. B., Anastasaki, C., Bian, S., Fine, H. A., Koga, T., Le, L. Q., Rodriguez, F. J., & Gutmann, D. H. (2024) · Disease Models & Mechanisms · doi:10.1242/dmm.050533 · Cited by 1
Mapping cell diversity in human sporadic cerebral cavernous malformations
Hou, X., Liang, F., Li, J., Yang, Y., Wang, C., Qi, T., & Sheng, W. (2024) · Gene · doi:10.1016/j.gene.2024.148605 · Cited by 1
DifferentialRegulation : a Bayesian hierarchical approach to identify differentially regulated genes
Tiberi, S., Meili, J., Cai, P., Soneson, C., He, D., Sarkar, H., Avalos‐Pacheco, A., Patro, R., & Robinson, M. D. (2024) · Biostatistics · doi:10.1093/biostatistics/kxae017 · Cited by 1
Chimeric brain organoids capture human genetic diversity
Bhaduri, A. (2024) · Nature · doi:10.1038/d41586-024-01648-7 · Cited by 1
Stem Cell-Derived Neural Organoids: From the Origin to Next Generation
Sakaguchi, H., & Takata, N. (2024) · Springer eBooks · doi:10.1007/978-981-99-7119-0_6 · Cited by 1
Pathway to independence: perspectives on the future
Birtele, M., Cerise, M., Djenoune, L., Kale, G., Maniou, E., Prahl, L. S., Schuster, K., & Villeneuve, C. (2024) · Development · doi:10.1242/dev.204366 · Cited by 1
Cerebral organoid research for pediatric patients with neurological disorders
Eun, J., Lee, J. E., & Yang, S.-H. (2024) · Korean Journal of Pediatrics · doi:10.3345/cep.2024.01235 · Cited by 1
Handcock, S. A., Richards, K., Karle, T. J., Kairath, P., Soch, A., Chavez, C. A., Petrou, S., & Maljevic, S. (2025) · Organoids · doi:10.3390/organoids4010001 · Cited by 1
González-Sastre, R., Coronel, R., Mateos-Martínez, P., Maeso, L., Llorente-Beneyto, E., Luque, A., Anta, B., López-Alonso, V., & Liste, I. (2025) · STAR Protocols · doi:10.1016/j.xpro.2025.103678 · Cited by 2
Age-dependent integration of cortical progenitors transplanted at CSF-neurogenic niche interface
Pothayee, N., Greene, G., Jahanipour, J., Jie, H., Tao-Cheng, J.-H., Petrus, E., Maric, D., & Koretsky, A. P. (2025) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2025.1577045 · Cited by 1
Modeling Down Syndrome Neurodevelopment with Dosage Compensation
Czerminski, J. T. (2019) · eScholarship@UMassChan · doi:10.13028/j7xf-1q21 · Cited by 1
Uncovering cell biology in the third dimension
Robertson, G. L., Romero-Morales, A. I., Lippmann, E. S., & Gama, V. (2020) · Molecular Biology of the Cell · doi:10.1091/mbc.e19-04-0211 · Cited by 1
Capturing complex epigenetic phenomena through human multicellular systems
Sen, D., & Keung, A. J. (2020) · Current Opinion in Biomedical Engineering · doi:10.1016/j.cobme.2020.05.008 · Cited by 1
Boeshore, K. L., Lee, C., & Freed, W. J. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-823882-0.00006-0 · Cited by 1
Modeling Schizophrenia In Vitro: Challenges and Insights on Studying Brain Cells
Brandão‐Teles, C., Zuccoli, G. S., Smith, B. J., Vieira, G. M., & Crunfli, F. (2022) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-97182-3_3 · Cited by 1
Cerebral Organoids in Developmental Neuroscience
Forero, A., & Cappello, S. (2022) · Springer eBooks · doi:10.1007/978-3-030-88832-9_179 · Cited by 1
Pain, B., Aurine, N., Rosa, T. L., Baquerre, C., & Coulpier, M. (2023) · INRAE Productions Animales · doi:10.20870/productions-animales.2023.36.2.7637 · Cited by 1
Fischer, J., Peters, J., Namba, T., Huttner, W. Β., & Heide, M. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.10.01.322792 · Cited by 1
Molecular profiling of retinal pigment epithelial cell differentiation for therapeutic usePreprint
Petrus-Reurer, S., Lederer, A. R., Baqué-Vidal, L., Douagi, I., Pannagel, B., Aronsson, M., Bartuma, H., Wagner, M., André, H., Sundström, E., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.01.31.429014 · Cited by 1
Glutamatergic dysfunction precedes neuron loss in cerebral organoids with MAPT mutationPreprint
Bowles, K. R., Silva, M., Whitney, K., Bertucci, T., Garza, J. C., Boles, N., Strang, K. H., Mahali, S. K., Marsh, J. A., Chen, C., et al. (2021) · bioRxiv · doi:10.1101/2021.02.03.429623 · Cited by 3
A Cultured Sensorimotor Organoid Model Forms Human Neuromuscular JunctionsPreprint
Pereira, J. D., DuBreuil, D. M., Devlin, A., Held, A., Sapir, Y., Berezovski, E., Hawrot, J., Dorfman, K., Chander, V., & Wainger, B. J. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.03.03.433128 · Cited by 1
Fernando, M., Lee, S. D., Wark, J. R., Xiao, D., Kim, H. J., Smith, G. C., Wong, T., Teber, E., Ali, R. R., Yang, P., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.05.16.444356 · Cited by 1
Karmirian, K., Goto‐Silva, L., Nascimento, J. M., Costa, M. N., Salerno, J. A., Ornelas, I. M., Vanderborght, B., Martins‐de‐Souza, D., & Rehen, S. K. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.06.16.448740 · Cited by 1
Yıldırım, M., Delepine, C., Feldman, D., Pham, V. A., Chou, S., Ip, J. P. K., Nott, A., Tsai, L., Ming, G., So, P. T. C., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.02.28.482282 · Cited by 1
He, C., Kalafut, N. C., Sandoval, S. O., Risgaard, R. D., Yang, C., Khullar, S., Suzuki, M., Chang, Q., Zhao, X., Sousa, A. M. M., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.06.13.495946 · Cited by 1
The biology of schizophrenia as a neurodevelopmental disorder
van Berlekom, A. B. (2022) · Utrecht University Repository (Utrecht University) · doi:10.33540/1269 · Cited by 1
Ezra, S., & Danter, W. R. (2022) · Preprints.org · doi:10.20944/preprints202210.0217.v1 · Cited by 1
Pigoni, M., Uzquiano, A., Paulsen, B. D. S., Kedaigle, A. J., Yang, S. M., Symvoulidis, P., Adiconis, X., Velasco, S., Sartore, R., Kim, K., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.11.15.516664 · Cited by 1
Self-citationGenetics of cortical development
Bella, D. J. D., & Habibi, E. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-818872-9.00108-4 · Cited by 1
Singh, J., Daniels, N. J., Pirozzi, F., Wynshaw-Boris, A. J., Lopez-Gonzalez, R., & Padgett, R. A. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.12.29.520610 · Cited by 1
Bury, L. A. D., Fu, S., & Wynshaw-Boris, A. J. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.06.17.545314 · Cited by 1
Microplastics Accumulation Induces Kynurenine-Derived Neurotoxicity in Cerebral OrganoidsPreprint
Park, S. B., Koh, B., Kim, S. S., Jung, W. H., Bae, M. A., & Kim, K. Y. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4576482 · Cited by 1
Ramani, A., Pasquini, G., Gerkau, N. J., Altinisk, N., Vinchure, O. S., Gabriel, E., Rothenaigner, I., Lin, S., Mariappan, A., Rathinam, D., et al. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4627469 · Cited by 1
Leptomeningeal Neural Organoid (LMNO) Fusions as Models to Study Meninges-Brain SignalingPreprint
Gama, V., Jones, H., Robertson, G. L., O’Rourke, R., Romero-Morales, A. I., & Siegenthaler, J. A. (2023) · Research Square · doi:10.21203/rs.3.rs-3694849/v1 · Cited by 1
Xu, Q., Wang, Y., He, Y., Zheng, X.-Q., Tong, M.-L., Yu, L., & Yang, T. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.01.23.576898 · Cited by 1
Zhang, J., Hu, G., Lu, Y., Ren, H., Huang, Y., Wen, Y., Ji, B., Wang, D., Wang, H., Liu, H., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.04.07.588438 · Cited by 1
Xu, Q., Wang, Y., He, Y., Zheng, X.-Q., Tong, M.-L., Yu, L., & Yang, T. (2024) · eLife · doi:10.7554/elife.95197.2 · Cited by 1
Metadata
- Authors
- Velasco, S., Kedaigle, A. J., Simmons, S. K., Nash, A., Rocha, M., Quadrato, G., Paulsen, B., Nguyen, L., Adiconis, X., Regev, A., Levin, J. Z., & Arlotta, P.
- Affiliations
- Broad Institute (US)
- Corresponding author
- Arlotta, Paola
- ORCID
- Venue
- Nature
- Published
- 2019-06
- Volume
- 570
- Issue
- 7762
- Pages
- 523-527
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC6906116
- OpenAlex
- W2949020598
- Cited by
- 1,094
- References
- 39
- Citations by year
- 2026: 145 · 2025: 156 · 2024: 184 · 2023: 131 · 2022: 148 · 2021: 142 · 2020: 150 · 2019: 35
- FWCI
- 44.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (acceptedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- Broad Institute
- Stanley Center for Psychiatric Research, Broad Institute
- Klarman Cell Observatory, Broad Institute
- National Institute of Mental Health (P50MH094271, U01MH115727, R01MH112940)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Keywords
- human brain organoids, cortical development, cell type diversity, dorsal forebrain, stem cell origin, reproducibility, single-cell transcriptomics
- MeSH terms
- RNA-Seq, Cell Line, Cerebral Cortex, Female, Fetus, Humans, Male, Organoids, Time Factors, Reproducibility of Results, Prosencephalon, Tissue Culture Techniques, Induced Pluripotent Stem Cells, Single-Cell Analysis, Transcriptome
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
