The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Human blastoids model blastocyst development and implantation
Open accessAbstract
One week after fertilization, human embryos implant into the uterus. This event requires the embryo to form a blastocyst consisting of a sphere encircling a cavity lodging the embryo proper. Stem cells can form a blastocyst model that we called a blastoid 1 . Here we show that naive human pluripotent stem cells cultured in PXGL medium 2 and triply inhibited for the Hippo, TGF-β and ERK pathways efficiently (with more than 70% efficiency) form blastoids generating blastocyst-stage analogues of the three founding lineages (more than 97% trophectoderm, epiblast and primitive endoderm) according to the sequence and timing of blastocyst development. Blastoids spontaneously form the first axis, and we observe that the epiblast induces the local maturation of the polar trophectoderm, thereby endowing blastoids with the capacity to directionally attach to hormonally stimulated endometrial cells, as during implantation. Thus, we propose that such a human blastoid is a faithful, scalable and ethical model for investigating human implantation and development 3,4 .
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Cites
Full-length RNA-seq from single cells using Smart-seq2
Picelli, S., Faridani, O. R., Björklund, Å. K., Winberg, G., Sagasser, S., & Sandberg, R. (2014) · Nature Protocols · doi:10.1038/nprot.2014.006 · Cited by 4,137
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Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer
Gardner, D. K., Lane, M., Stevens, J., Schlenker, T., & Schoolcraft, W. B. (2000) · Fertility and Sterility · doi:10.1016/s0015-0282(00)00518-5 · Cited by 2,091
Regulation of the Hippo-YAP Pathway by G-Protein-Coupled Receptor Signaling
Yu, F.-X., Zhao, B., Panupinthu, N., Jewell, J. L., Lian, I., Wang, L. H., Zhao, J., Yuan, H., Tumaneng, K., Li, H., et al. (2012) · Cell · doi:10.1016/j.cell.2012.06.037 · Cited by 1,628
Single-Cell RNA-Seq Reveals Lineage and X Chromosome Dynamics in Human Preimplantation Embryos
Petropoulos, S., Edsgärd, D., Reinius, B., Deng, Q., Panula, S. P., Codeluppi, S., Plaza Reyes, A., Linnarsson, S., Sandberg, R., & Lanner, F. (2016) · Cell · doi:10.1016/j.cell.2016.03.023 · Cited by 1,063
Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human
Takashima, Y., Guo, G., Loos, R., Nichols, J., Ficz, G., Krueger, F., Oxley, D., Santos, F., Clarke, J., Mansfield, W., et al. (2014) · Cell · doi:10.1016/j.cell.2014.08.029 · Cited by 952
Derivation of Human Trophoblast Stem Cells
Okae, H., Toh, H., Sato, T., Hiura, H., Takahashi, S., Shirane, K., Kabayama, Y., Suyama, M., Sasaki, H., & Arima, T. (2018) · Cell Stem Cell · doi:10.1016/j.stem.2017.11.004 · Cited by 903
Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium
Turco, M. Y., Gardner, L., Hughes, J., Cindrova-Davies, T., Gomez, M. J., Farrell, L., Hollinshead, M., Marsh, S. G. E., Brosens, J. J., Critchley, H. O., et al. (2017) · Nature Cell Biology · doi:10.1038/ncb3516 · Cited by 789
Trophoblast organoids as a model for maternal–fetal interactions during human placentation
Turco, M. Y., Gardner, L., Kay, R. G., Hamilton, R. S., Prater, M., Hollinshead, M. S., McWhinnie, A., Esposito, L., Fernando, R., Skelton, H., et al. (2018) · Nature · doi:10.1038/s41586-018-0753-3 · Cited by 694
Single-cell transcriptomic atlas of the human endometrium during the menstrual cycle
Wang, W., Vilella, F., Alama, P., Moreno, I., Mignardi, M., Isakova, A., Pan, W., Simon, C., & Quake, S. R. (2020) · Nature Medicine · doi:10.1038/s41591-020-1040-z · Cited by 614
Cited by
Induced pluripotent stem cells (iPSCs): molecular mechanisms of induction and applications
Cerneckis, J., Cai, H., & Shi, Y. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-024-01809-0 · Cited by 452
Potential Autoimmunity Resulting from Molecular Mimicry between SARS-CoV-2 Spike and Human Proteins
Nunez-Castilla, J., Stebliankin, V., Baral, P., Balbin, C. A., Sobhan, M., Cickovski, T., Mondal, A. M., Narasimhan, G., Chapagain, P., Mathee, K., et al. (2022) · Viruses · doi:10.3390/v14071415 · Cited by 100
Jamaluddin, M. F. B., Ghosh, A., Ingle, A., Mohammed, R., Ali, A., Bahrami, M., Kaiko, G., Gibb, Z., Filipe, E. C., Cox, T. R., et al. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2208040119 · Cited by 84
Metadata
- Authors
- Kagawa, H., Javali, A., Khoei, H. H., Sommer, T. M., Sestini, G., Novatchkova, M., Scholte op Reimer, Y., Castel, G., Bruneau, A., Maenhoudt, N., Lammers, J., Loubersac, S., Freour, T., Vankelecom, H., David, L., & Rivron, N.
- Venue
- Nature
- Published
- 2021-12-02
- Volume
- 601
- Issue
- 7894
- Pages
- 600-605
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W3217061287
- Cited by
- 498
- References
- 48
- FWCI
- 20.80
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Kagawa, H., Javali, A., Khoei, H. H., Sommer, T. M., Sestini, G., Novatchkova, M., Scholte op Reimer, Y., Castel, G., Bruneau, A., Maenhoudt, N., Lammers, J., Loubersac, S., Freour, T., Vankelecom, H., David, L., & Rivron, N. (2022). Human blastoids model blastocyst development and implantation. Nature. https://doi.org/10.1038/s41586-021-04267-8
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