Cited by 103Immune cells in cancerMesenchymal stem cell researchT-cell and B-cell Immunology

The DOI matches its Crossref record; 56 of the 57 references it cites were found in a registry or an index.

Long-term culture-expanded alveolar macrophages restore their full epigenetic identity after transfer in vivo

Open access

Subramanian, Sethuraman, Busch, Clara Jana-Lui, Molawi, Kaaweh, Geirsdottir, Laufey, Maurizio, Julien, Vargas Aguilar, Stephanie, Belahbib, Hassiba, Gimenez, Gregory, Yuda, Ridzky Anis Advent, Burkon, Michaela, Favret, Jérémy, Gholamhosseinian Najjar, Sara, de Laval, Bérengère, Kandalla, Prashanth Kumar, Sarrazin, Sandrine, Alexopoulou, Lena, Sieweke, Michael H.·2022·Nature Immunology

DOI 10.1038/s41590-022-01146-w

Published version of a preprint

Open this paper in CoChat — free account Open PDF

Abstract

Alveolar macrophages (AMs) are lung tissue-resident macrophages that can be expanded in culture, but it is unknown to what extent culture affects their in vivo identity. Here we show that mouse long-term ex vivo expanded AMs (exAMs) maintained a core AM gene expression program, but showed culture adaptations related to adhesion, metabolism and proliferation. Upon transplantation into the lung, exAMs reacquired full transcriptional and epigenetic AM identity, even after several months in culture and could self-maintain long-term in the alveolar niche. Changes in open chromatin regions observed in culture were fully reversible in transplanted exAMs and resulted in a gene expression profile indistinguishable from resident AMs. Our results indicate that long-term proliferation of AMs in culture did not compromise cellular identity in vivo. The robustness of exAM identity provides new opportunities for mechanistic analysis and highlights the therapeutic potential of exAMs.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1038/s41590-022-01146-w

Cites

56 of 56 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities
Heinz, S., Benner, C., Spann, N., Bertolino, E., Lin, Y. C., Laslo, P., Cheng, J. X., Murre, C., Singh, H., & Glass, C. K. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.05.004 · Cited by 13,382
GREAT improves functional interpretation of cis-regulatory regions
McLean, C. Y., Bristor, D., Hiller, M., Clarke, S. L., Schaar, B. T., Lowe, C. B., Wenger, A. M., & Bejerano, G. (2010) · Nature Biotechnology · doi:10.1038/nbt.1630 · Cited by 4,502
Pathological inflammation in patients with COVID-19: a key role for monocytes and macrophages
Merad, M., & Martin, J. C. (2020) · Nature Reviews Immunology · doi:10.1038/s41577-020-0331-4 · Cited by 2,472
mTOR- and HIF-1α–mediated aerobic glycolysis as metabolic basis for trained immunity
Cheng, S.-C., Quintin, J., Cramer, R. A., Shepardson, K. M., Saeed, S., Kumar, V., Giamarellos-Bourboulis, E. J., Martens, J. H. A., Rao, N. A., Aghajanirefah, A., et al. (2014) · Science · doi:10.1126/science.1250684 · Cited by 2,224
Transcriptome-Based Network Analysis Reveals a Spectrum Model of Human Macrophage Activation
Xue, J., Schmidt, S. V., Sander, J., Draffehn, A., Krebs, W., Quester, I., De Nardo, D., Gohel, T. D., Emde, M., Schmidleithner, L., et al. (2014) · Immunity · doi:10.1016/j.immuni.2014.01.006 · Cited by 2,210
Tissue-Resident Macrophage Enhancer Landscapes Are Shaped by the Local Microenvironment
Lavin, Y., Winter, D., Blecher-Gonen, R., David, E., Keren-Shaul, H., Merad, M., Jung, S., & Amit, I. (2014) · Cell · doi:10.1016/j.cell.2014.11.018 · Cited by 2,167
Tissue-resident macrophages
Davies, L. C., Jenkins, S. J., Allen, J. E., & Taylor, P. R. (2013) · Nature Immunology · doi:10.1038/ni.2705 · Cited by 2,096
Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages
the Immunological Genome Consortium, Gautier, E. L., Shay, T., Miller, J., Greter, M., Jakubzick, C., Ivanov, S., Helft, J., Chow, A., Elpek, K. G., et al. (2012) · Nature Immunology · doi:10.1038/ni.2419 · Cited by 1,972
Epigenetic programming of monocyte-to-macrophage differentiation and trained innate immunity
Saeed, S., Quintin, J., Kerstens, H. H. D., Rao, N. A., Aghajanirefah, A., Matarese, F., Cheng, S.-C., Ratter, J., Berentsen, K., van der Ent, M. A., et al. (2014) · Science · doi:10.1126/science.1251086 · Cited by 1,761
Human chimeric antigen receptor macrophages for cancer immunotherapy
Klichinsky, M., Ruella, M., Shestova, O., Lu, X. M., Best, A., Zeeman, M., Schmierer, M., Gabrusiewicz, K., Anderson, N. R., Petty, N. E., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0462-y · Cited by 1,495

Show all 57 references

Research works printed but not linked: 1

Lim, W. A. & June, C. H. The principles of engineering immune. Cells Treat. Cancer Cell 168, 724–740 (2017).

Sorted by citations

Biology of lung macrophages in health and disease
Aegerter, H., Lambrecht, B. N., & Jakubzick, C. (2022) · Immunity · doi:10.1016/j.immuni.2022.08.010 · Cited by 656
An integrated view of anti-inflammatory and antifibrotic targets for the treatment of NASH
Tacke, F., Puengel, T., Loomba, R., & Friedman, S. L. (2023) · Journal of Hepatology · doi:10.1016/j.jhep.2023.03.038 · Cited by 321
Alveolar macrophages in tissue homeostasis, inflammation, and infection: evolving concepts of therapeutic targeting
Malainou, C., Abdin, S. M., Lachmann, N., Matt, U., & Herold, S. (2023) · Journal of Clinical Investigation · doi:10.1172/jci170501 · Cited by 215
Inducible antibacterial responses in macrophages
Sweet, M. J., Ramnath, D., Singhal, A., & Kapétanovic, R. (2024) · Nature reviews. Immunology · doi:10.1038/s41577-024-01080-y · Cited by 171
Clinical landscape of macrophage-reprogramming cancer immunotherapies
Rannikko, J. H., & Hollmén, M. (2024) · British Journal of Cancer · doi:10.1038/s41416-024-02715-6 · Cited by 169
Methylation across the central dogma in health and diseases: new therapeutic strategies
Liu, R., Zhao, E., Yu, H., Yuan, C., Abbas, M. N., & Cui, H. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01528-y · Cited by 155
Macrophages: Subtypes, Distribution, Polarization, Immunomodulatory Functions, and Therapeutics
Peng, M., Li, N., Wang, H., Li, Y., Liu, H., Luo, Y., Lang, B., Zhang, W., Li, S., Tian, L., et al. (2025) · MedComm · doi:10.1002/mco2.70304 · Cited by 98
Unraveling the enigma of tumor-associated macrophages: challenges, innovations, and the path to therapeutic breakthroughs
Shao, S., Miao, H., & Ma, W. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1295684 · Cited by 67
Intestinal microbiota programming of alveolar macrophages influences severity of respiratory viral infection
Ngo, V. L., Lieber, C. M., Kang, H., Sakamoto, K., Kuczma, M., Plemper, R. K., & Gewirtz, A. T. (2024) · Cell Host & Microbe · doi:10.1016/j.chom.2024.01.002 · Cited by 66
Targeted macrophage phagocytosis by Irg1/itaconate axis improves the prognosis of intracerebral hemorrhagic stroke and peritonitis
Luo, Z., Sheng, Z., Hu, L., Shi, L., Tian, Y., Zhao, X., Yang, W., Xiao, Z., Shen, D., Wu, W., et al. (2024) · EBioMedicine · doi:10.1016/j.ebiom.2024.104993 · Cited by 60
Lipoxin Mimetics and the Resolution of Inflammation
Godson, C., Guiry, P. J., & Brennan, E. P. (2023) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev-pharmtox-051921-085407 · Cited by 59
Camouflaging attenuated Salmonella by cryo-shocked macrophages for tumor-targeted therapy
Wu, L., Du, Z., Li, L., Qiao, L., Zhang, S., Yin, X., Chang, X., Li, C., & Hua, Z. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01703-1 · Cited by 57
Exosomal STIMATE derived from type II alveolar epithelial cells controls metabolic reprogramming of tissue-resident alveolar macrophages
Feng, Z., Zhou, J., Li, Q., Chu, L., Jiang, Y., Zhang, X., Yan, L., Liu, Y., Jiang, J. H., Xu, P., et al. (2023) · Theranostics · doi:10.7150/thno.82552 · Cited by 50
Liver macrophages revisited: The expanding universe of versatile responses in a spatiotemporal context
Guillot, A., & Tacke, F. (2024) · Hepatology Communications · doi:10.1097/hc9.0000000000000491 · Cited by 45
Glypican-3-targeted macrophages delivering drug-loaded exosomes offer efficient cytotherapy in mouse models of solid tumours
Liu, J., Zhao, H., Gao, T., Huang, X., Liu, S., Liu, M., Mu, W., Liang, S., Fu, S., Yuan, S., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-52500-5 · Cited by 43
Deep proteomic analysis of microglia reveals fundamental biological differences between model systems
Lloyd, A. F., Martínez‐Muriana, A., Davis, E., Daniels, M. J. D., Hou, P., Mancuso, R., Brenes, A. J., Sinclair, L. V., Geric, I., Snellinx, A., et al. (2024) · Cell Reports · doi:10.1016/j.celrep.2024.114908 · Cited by 47
A new tractable method for generating human alveolar macrophage-like cells in vitro to study lung inflammatory processes and diseases
Pahari, S., Arnett, E., Simper, J., Azad, A., Guerrero‐Arguero, I., Ye, C., Zhang, H., Cai, H., Wang, Y., Lai, Z., et al. (2023) · mBio · doi:10.1128/mbio.00834-23 · Cited by 33
Interactions of Inhaled Liposome with Macrophages and Neutrophils Determine Particle Biofate and Anti-Inflammatory Effect in Acute Lung Inflammation
Liu, C., Liu, Y., Xi, L. F., He, Y., Liang, Y., Mak, J., Mao, S., Wang, Z., & Zheng, Y. (2022) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.2c17660 · Cited by 33
Therapeutic modulation of the liver immune microenvironment
Tilg, H., Adolph, T. E., & Tacke, F. (2023) · Hepatology · doi:10.1097/hep.0000000000000386 · Cited by 32
The impact of the tumor microenvironment on macrophages
Xiao, M., & Li, X. (2025) · Frontiers in Immunology · doi:10.3389/fimmu.2025.1572764 · Cited by 28
Single cell RNA sequencing unravels mechanisms underlying senescence-like phenotypes of alveolar macrophages
Wu, Y., Hu, S. S., Zhang, R., Goplen, N. P., Gao, X., Narasimhan, H., Shi, A., Chen, Y. M., Li, Y., Zang, C., et al. (2023) · iScience · doi:10.1016/j.isci.2023.107197 · Cited by 25
CircCDC42-encoded CDC42-165aa regulates macrophage pyroptosis in Klebsiella pneumoniae infection through Pyrin inflammasome activation
Xu, N., Jiang, J., Jiang, F., Dong, G., Meng, L., Wang, M., Chen, J., Li, C., Shi, Y., He, S., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-50154-x · Cited by 25
Alveolar Macrophages in Viral Respiratory Infections: Sentinels and Saboteurs of Lung Defense
Pöpperl, P., Stoff, M., & Beineke, A. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26010407 · Cited by 21
Human liver immunology: from in vitro models to new insights
Rezvani, M. (2025) · Cellular and Molecular Immunology · doi:10.1038/s41423-025-01312-8 · Cited by 20
Gut microbial GABAergic signaling improves stress-associated innate immunity to respiratory viral infection
Gao, Y., Liang, Z., Mao, B., Zheng, X., Shan, J., Jin, C., Liu, S., Kolliputi, N., Chen, Y., Xu, F., et al. (2023) · Journal of Advanced Research · doi:10.1016/j.jare.2023.06.008 · Cited by 20
Differences in glycolytic metabolism between tissue-resident alveolar macrophages and recruited lung macrophages
Woods, P. S., & Mutlu, G. M. (2025) · Frontiers in Immunology · doi:10.3389/fimmu.2025.1535796 · Cited by 21
Innate type 2 lymphocytes trigger an inflammatory switch in alveolar macrophages
Verwaerde, S., Hastir, J., Schetters, S. T. T., Smole, U., Seys, L., Baptista, A. P., English, K., Schuijs, M. J., Aegerter, H., Damme, K. F. A. V., et al. (2025) · Immunity · doi:10.1016/j.immuni.2025.11.015 · Cited by 20
Trained immunity and epigenetic memory in long-term self-renewing hematopoietic cells
Johansson, A., Lin, D. S. Y., Mercier, F., Yamashita, M., Divangahi, M., & Sieweke, M. H. (2023) · Experimental Hematology · doi:10.1016/j.exphem.2023.02.001 · Cited by 17
Self-citation
Epigenetic regulation of macrophage activation in chronic obstructive pulmonary disease
Zhang, F., Cui, Y., Zhang, T., & Yin, W. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1445372 · Cited by 17
Adoptive transfer of Fe3O4-SWCNT engineered M1-like macrophages for magnetic resonance imaging and enhanced cancer immunotherapy
Zhang, Y., Liu, S.-W., Li, D., He, C., Wang, D., Wei, M., Zheng, S., & Li, J. (2023) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2023.113452 · Cited by 16
Crystalline silica-induced proinflammatory eicosanoid storm in novel alveolar macrophage model quelled by docosahexaenoic acid supplementation
Favor, O. K., Rajasinghe, L. D., Wierenga, K. A., Maddipati, K. R., Lee, K. S. S., Olive, A. J., & Pestka, J. J. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1274147 · Cited by 14
Epigenetic Control of Alveolar Macrophages: Impact on Lung Health and Disease
Parajuli, N., Subedi, K., Solone, X. K., Jiang, A., Zhou, L., & Mi, Q. (2025) · Cells · doi:10.3390/cells14090640 · Cited by 15
Polystyrene microplastic-induced pathophysiology is driven by disruption of efferocytosis
Codo, A. C., Romero-Pichardo, J. E., Wang, Z., Aufiero, M. A., Lazarov, T., Rojas, W. S., Walker, N. S., Nair, A., Cole, R. F., Adkins, S., et al. (2026) · Immunity · doi:10.1016/j.immuni.2026.01.009 · Cited by 14
HDM induces distinct immunometabolic phenotype in macrophages in TLR4-dependent manner
Yurakova, T. R., Gubernatorova, E. O., Gorshkova, E. A., Nosenko, M. A., Nedospasov, S. A., & Drutskaya, M. S. (2022) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2022.166531 · Cited by 13
Optimized method for higher yield of alveolar macrophage isolation for ex vivo studies
Devkota, S. P., Onah, C., Joshi, P. R., Adhikari, S., & Baral, P. (2024) · Heliyon · doi:10.1016/j.heliyon.2024.e37221 · Cited by 12
Towards using 3D cellular cultures to model the activation and diverse functions of macrophages
Cutter, S. W., Wright, M. D., Reynolds, N. P., & Binger, K. J. (2023) · Biochemical Society Transactions · doi:10.1042/bst20221008 · Cited by 12
Alleviation of monocyte exhaustion by BCG derivative mycolic acid
Wu, Y., Caldwell, B. A., Wang, J., Zhang, Y., & Li, L. (2024) · iScience · doi:10.1016/j.isci.2024.108978 · Cited by 9
PPAR-γ suppresses macrophage senescence and allergic airway inflammation through controlling lipid metabolic pathways
Gu, W., Wan, R., Chen, Z., Wang, W., Xie, S., Hao, C., Liu, G., Wan, M., & Gao, P. (2026) · EBioMedicine · doi:10.1016/j.ebiom.2026.106226 · Cited by 12
The crosstalk between parenchymal cells and macrophages: A keeper of tissue homeostasis
Chen, Y., & Tang, L. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.1050188 · Cited by 11
Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages
Busch, C. J., Subramanian, S., Linares, J., Favret, J., Yuda, R. A. A., & Sieweke, M. H. (2023) · Methods in molecular biology · doi:10.1007/978-1-0716-3437-0_16 · Cited by 9
Self-citation
Dynamic Multiplex Tissue Imaging in Inflammation Research
Uderhardt, S., Neag, G., & Germain, R. N. (2023) · Annual Review of Pathology Mechanisms of Disease · doi:10.1146/annurev-pathmechdis-070323-124158 · Cited by 10
Macrophage sensitivity to bexmarilimab-induced reprogramming is shaped by the tumor microenvironment
Rannikko, J. H., Turpin, R., Boström, P., Virtakoivu, R., Harth, C., Takeda, A., Tamminen, A., Koskivuo, I., & Hollmén, M. (2025) · Journal for ImmunoTherapy of Cancer · doi:10.1136/jitc-2024-011292 · Cited by 9
Deep proteomic analysis of microglia reveals fundamental biological differences between model systemsPreprint
Lloyd, A. F., Martínez‐Muriana, A., Davis, E., Daniels, M. J. D., Hou, P., Mancuso, R., Brenes, A. J., Geric, I., Snellinx, A., Craessaerts, K., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.07.07.498804 · Cited by 9
Bacterial pneumonia induces senescence in resident alveolar macrophages that are outcompeted by monocytes
Zhang, J., Wang, T., Wang, Y., Li, Y., Wang, L., Wang, J., Miao, Y., Xu, F., & Yao, Y. (2025) · Cell Reports · doi:10.1016/j.celrep.2025.115571 · Cited by 8
Pathogenicity and virulence of Mycobacterium abscessus
Soverina, S., Gilliland, H. N., & Olive, A. J. (2025) · Virulence · doi:10.1080/21505594.2025.2508813 · Cited by 9
Role of trained innate immunity against mucosal cancer
Wang, T., Wang, Y., Zhang, J., & Yao, Y. (2024) · Current Opinion in Virology · doi:10.1016/j.coviro.2024.101387 · Cited by 8
Sensing of metabolic signals via GPR183 promotes occupation of lung macrophage niches by monocytes
Bub, L., Evren, E., Verwaerde, S., Ruscitti, C., Vanneste, D., Ghosh, P., Gao, Y., Sleiers, N., Deng, R., Montes, M. L., et al. (2026) · The Journal of Experimental Medicine · doi:10.1084/jem.20252667 · Cited by 7
GSK3α/β Restrain IFN-γ–Inducible Costimulatory Molecule Expression in Alveolar Macrophages, Limiting CD4+ T Cell Activation
Ankley, L. M., Conner, K. N., Vielma, T. E., Godfrey, J. J., Thapa, M., & Olive, A. J. (2024) · ImmunoHorizons · doi:10.4049/immunohorizons.2300107 · Cited by 5
Differential regulation of lung homeostasis and silicosis by the TAM receptors MerTk and Axl
Pinto, K. G., Leandro, M., Corrêa, A., Maia, E. P., Rodrigues, L., da Costa, A. L. A., Ferreira, J. R. M., Claudio-Etienne, E., Siebenlist, U. K., He, J., et al. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1380628 · Cited by 7
Integrated multi‐omics profiling landscape of organising pneumonia
Tang, Y., Chu, C., Bu, S., Sun, Q., Liu, A., Xie, J., Qiao, S., Huang, L., & Wang, H. (2024) · Clinical and Translational Medicine · doi:10.1002/ctm2.1782 · Cited by 7
Disruption of immune responses by type 1 diabetes exacerbates SARS-CoV-2 mediated lung injury
Kass-Gergi, S., Gan, Z., Wong, J., Weiner, A. I., Adams‐Tzivelekidis, S., Gentile, M. E., Mendoza, M., Holcomb, N. P., Li, X., Singh, M., et al. (2024) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00250.2024 · Cited by 7
Absence of c-Maf and IL-10 enables type I IFN enhancement of innate responses to LPS in alveolar macrophages
Lim, P. N., Cervantes, M. M., Pham, L. K., Doherty, S. R., Tufts, A., Dubey, D., Mai, D., Aderem, A. A., Diercks, A. H., & Rothchild, A. C. (2025) · The Journal of Immunology · doi:10.1093/jimmun/vkae029 · Cited by 6
Evaluating the evidence for GM-CSF as a host-directed therapy in respiratory infections
David, C., Verney, C., Si‐Tahar, M., & Guillon, A. (2025) · Cytokine · doi:10.1016/j.cyto.2025.156902 · Cited by 7
iPSC-derived macrophages: An in vitro model to study human disease-relevant macrophage biology
Sauer, J., Schloesser, D., Koeppen, K., Fundel‐Clemens, K., Simone-Roach, C., Hall, K., Ivovic, A., Makings, J., Reiss, D., Traub, M., et al. (2025) · The Journal of Immunology · doi:10.1093/jimmun/vkaf184 · Cited by 6
Administration of amniotic fluid stem cell extracellular vesicles promotes development of fetal hypoplastic lungs by immunomodulating lung macrophagesPreprint
Antounians, L., Figueira, R. L., Kukreja, B., Zani‐Ruttenstock, E., Khalaj, K., Montalva, L., Doktor, F., Obed, M., Blundell, M., Wu, T., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.11.29.518388 · Cited by 6
Pelargonidin-3-O-galactoside Alleviates Acute Lung Injury Induced by Klebsiella pneumoniae by Targeting CD38-Mediated Pyroptosis
Jiang, F., Jiang, J., Sun, Y., Li, M., Chen, J., Liang, L., Wang, X., Zhang, H., Meng, M., Yuan, B., et al. (2025) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.4c10129 · Cited by 5
Phosphopeptides P140 cause oxidative burst responses of pulmonary macrophages in an imiquimod-induced lupus model
Zhong, J., Zheng, C., Chen, Z., Yue, H., Gao, H., Jiang, Y., Hui, H., & Tian, J. (2023) · Molecular Biomedicine · doi:10.1186/s43556-023-00149-9 · Cited by 5
A comprehensive evaluation of murine and humanex vivocultured alveolar macrophages
David, C., Bréa, D., Verney, C., Cezard, A., Vasseur, V., Briard, B., Khau, S., Barsac, E., Ferreira, M., Marchand-Adam, S., et al. (2024) · ERJ Open Research · doi:10.1183/23120541.00859-2024 · Cited by 4
Bronchoalveolar lavage fluid (BALF): Clinical applications for present and future
Mei, J., Chen, Y.-S., Tian, H., Wang, L., Zeng, J., Wang, L., Rong, D., Wong, R. C. B., Gao, Y., Guo, C., et al. (2025) · The Innovation Medicine · doi:10.59717/j.xinn-med.2025.100115 · Cited by 4
Macrophages with different origins proliferate ex vivo and do not lose their core intrinsic features
Habash, S. A., Takahashi, N., Eltalkhawy, Y. M., Abdelnaser, R. A., Ogata-Aoki, H., Okada, S., Takizawa, H., Usuki, S., Etoh, K., Hino, S., et al. (2025) · iScience · doi:10.1016/j.isci.2025.112635 · Cited by 4
Sexual dimorphism of lung immune-regulatory units imprint biased pulmonary fibrosis
Peng, X. F., Wang, L., Xu, F., Guo, Q., Xie, G., Sheng, L., Chen, L., Teng, J., Yang, J., Wu, X., et al. (2025) · Cellular and Molecular Immunology · doi:10.1038/s41423-025-01293-8 · Cited by 6
Thyroid hormone deficiency worsens outcomes in vaccinia virus infection
Notario, L., Guerrero-Espinosa, E., Nistal, M., Lauzurica, P., Aranda, A., & Alemany, S. (2025) · Journal of Virology · doi:10.1128/jvi.01294-25 · Cited by 4
Engineered Strategies to Interfere with Macrophage Fate in Myocardial Infarction
Zhai, Z., Yang, C., Yin, W., Liu, Y., Li, S., Ye, Z., Xie, M., & Song, X. (2025) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.4c02061 · Cited by 4
Mycobacterium tuberculosis triggers reduced inflammatory cytokine responses and virulence in mice lacking Tax1bp1
Chin, J., Abeydeera, N., Repasy, T., Rivera‐Lugo, R., Mitchell, G., Nguyen, V. Q., Zheng, W., Richards, A., Stevenson, E. J., Swaney, D. L., et al. (2025) · PLoS Pathogens · doi:10.1371/journal.ppat.1012829 · Cited by 5
Tissue signatures of human macrophages during homeostasis and activation
Caron, D. P., Specht, W. L., Nargund, S., Chen, D., Wells, S. B., Hwu, J., Szabo, P. A., Sims, P. A., & Färber, D. L. (2025) · The Journal of Immunology · doi:10.1093/jimmun/vkaf317 · Cited by 3
Metabolic regulation of macrophage-tissue interactions in the lung
Willinger, T. (2026) · Mucosal Immunology · doi:10.1016/j.mucimm.2026.03.010 · Cited by 3
Alveolar macrophages inhibit emphysematous pathology via expression of carbonic anhydrase 4
Ponessa, J. J., Jianya, P., Schroeter, M. N., Sawhney, A., Chavan, K., Inclan-Rico, J. M., Espinosa, V., Chen, F., Shubin, N. J., Lung, T. W. F., et al. (2026) · Cell Reports · doi:10.1016/j.celrep.2026.117315 · Cited by 2
A signal-responsive cooperative transcription factor network determines alveolar macrophage identity
Mínguez-Martínez, J., Porcuna, J., Casablanca, L., Gómez, M. J., Aghelpasand, H., Höjer, P., García-Fojeda, B., Nuñez, V., Torroja, C., Mühleder, S., et al. (2026) · The Journal of Experimental Medicine · doi:10.1084/jem.20242085 · Cited by 2
Synergy between pluripotent stem cell‐derived macrophages and self‐renewing macrophages: Envisioning a promising avenue for the modelling and cell therapy of infectious diseases
Peng, D., Li, M., Yu, Z., Yan, T., Meng, Y., Su, L., Liu, Z. H., Li, L., & Qiu, H. (2024) · Cell Proliferation · doi:10.1111/cpr.13770 · Cited by 2
Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection
Durazo-Martínez, K., Chaudhari, J., Kumari, S., & Vu, H. L. X. (2024) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1505900 · Cited by 2
Expression of non-phosphorylatable S5A-L-plastin exerts phenotypes distinct from L-plastin deficiency during podosome formation and phagocytosis
Lin, X., Krishnamoorthy, P., Walker, E. C., Joshi, H. P., & Morley, S. C. (2023) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2023.1020091 · Cited by 2
Adaptation of Human iPSC-Derived Macrophages Toward an Alveolar Macrophage-Like Phenotype Post-Intra-Pulmonary Transfer into Murine Models
Hetzel, M., Gensch, I., Ackermann, M., & Lachmann, N. (2023) · Methods in molecular biology · doi:10.1007/978-1-0716-3437-0_31 · Cited by 1
Modulation of M1 macrophage polarization by Fe3O4@Cu2-xS engineering for improved tumor immunotherapy and MRI
Shi, C., Zhu, Y., Wang, Y., Zhao, N., Zhang, Z., Wang, Y., Xie, M., Hou, P., & Li, J. (2026) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2026.115614 · Cited by 1
singIST: An integrative method for comparative single-cell transcriptomics between disease models and humans
Moruno-Cuenca, A., Picart‐Armada, S., Wasikowski, R., Fox, J. P., Tsoi, L. C., Gudjonsson, J. E., Perera-Lluna, A., & Fernández-Albert, F. (2026) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1014002 · Cited by 1
The type-I interferon priming signal balances antibacterial and antitumor trained immunity in alveolar macrophages
Wang, T., Zhang, J., Li, Y., Miao, Y., Wang, Y., Wang, L., Liu, Y., Chang, C.-W., Chen, E., & Yao, Y. (2026) · Cellular and Molecular Immunology · doi:10.1038/s41423-026-01397-9 · Cited by 2
Modulation of alveolar macrophages by sex as a biological variable
Sonti, S., Leek, C. C., & Lingappan, K. (2026) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00410.2025 · Cited by 1
Defined culture conditions improve functional properties of mature iPSC-derived macrophages for therapeutic screening
Schinke, M., Gensch, I., Rietschel, T., Nguyen, A. H. H., Slevogt, H., Jänsch, L., & Lachmann, N. (2026) · Stem Cell Research & Therapy · doi:10.1186/s13287-026-05009-1 · Cited by 1
Acridine reimagined: from coal tar dye to biomedical catalyst and environmental guardian
Kujawa, J., Gharabli, S. A., Bronikowska, E., Obieziurska‐Fabisiak, M., Terzyk, A. P., Boncel, S., & Muzioł, T. M. (2026) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2026.218147 · Cited by 1
In situ reprogramming of CAR-alveolar macrophages via liposomal nanomedicine for lung cancer immunotherapy
Li, H., Lyu, C., Cai, X., Zhang, Y., Guo, Q., Chen, Y., Yang, C., Feng, Z., Zhang, Y., & Liu, J. (2026) · Nature Communications · doi:10.1038/s41467-026-74162-1 · Cited by 1
ILC2s govern imprinting of alveolar macrophage-mediated immune responses upon secondary helminth infection in the lung
Pollock, J., Patel, J. H., Graf, L.-M., Ruhl, A., Radtke, D., & Voehringer, D. (2026) · Mucosal Immunology · doi:10.1016/j.mucimm.2026.100367 · Cited by 1
Macrophage Polarisation During Leishmania ( Viannia ) braziliensis Infection in Mice
Silva, M. F. E., Roque, G. A. S., Coser, L. D. O., Jorge, G. P., de Oliveira, A. L. R., Rocha, F. J. S., & Giorgio, S. (2025) · Parasite Immunology · doi:10.1111/pim.70001 · Cited by 1
Absence of c-Maf and IL-10 enables Type I IFN enhancement of innate responses to low-dose LPS in alveolar macrophagesPreprint
Lim, P. N., Cervantes, M. M., Pham, L. K., Doherty, S., Tufts, A., Dubey, D., Mai, D., Aderem, A. A., Diercks, A. H., & Rothchild, A. C. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.05.22.594428 · Cited by 1
Intracellular C. neoformans infection stimulates increased glycolytic activity in fetal liver-derived alveolar-like macrophagesPreprint
Wiggins, D. A., Griggs, J. B., England, A., Callison, E. N., Kamel, C. H., Hasan, C. A., Davis, A. T., Chappell, M. E., Conner, K. N., Olive, A. J., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.11.28.690224 · Cited by 1
M-CSF drives alveolar macrophage plasticity during development and cytomegalovirus infectionPreprint
Baasch, S., Nelipovich, A., Zhumadilova, Z., Henschel, J., Ghanem, N., Döring, C.-L., Aktories, P., Wagner, L., Dey, A., Vanneste, D., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.04.11.717936 · Cited by 1
Single cell RNA sequencing reveals mechanisms underlying a senescence-like phenotype of Alveolar Macrophages during AgingPreprint
Wu, Y., Zhu, B., Zhang, R., Goplen, N. P., Gao, X., Narasimhan, H., Shi, A., Chen, Y. M., Li, Y., Dong, H., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.06.04.494822
Gut microbial GABAergic signaling imprints alveolar macrophages and pulmonary response to viral infection associated with psychological stressPreprint
Gao, Y., Liang, Z., Mao, B., Zheng, X., Shan, J., Jin, C., Liu, S., Kolliputi, N., Chen, Y., Xu, F., et al. (2022) · Research Square · doi:10.21203/rs.3.rs-2064240/v1
A Protective Role for the Lectin CD169/Siglec-1 During SARS-CoV-2 InfectionPreprint
Ullah, I., Ladinsky, M. S., Sharma, L., Gilani, Z., Rowland, E. F., Mack, M., Kieffer, C., Björkman, P. J., Mothes, W., Kumar, P., et al. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4514472
GSK3α/β restrains IFNγ-inducible costimulatory molecule expression in alveolar macrophages, limiting CD4 + T cell activationPreprint
Ankley, L. M., Conner, K. N., Vielma, T. E., Thapa, M., & Olive, A. J. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.08.16.553574
Tax1bp1 enhances bacterial virulence and promotes inflammatory responses during Mycobacterium tuberculosis infection of alveolar macrophagesPreprint
Chin, J., Abeydeera, N., Repasy, T., Rivera‐Lugo, R., Mitchell, G., Nguyen, V. Q., Zheng, W., Richards, A., Swaney, D. L., Krogan, N. J., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.12.16.628616
Type-I interferon priming signal balances anti-bacterial and anti-tumor trained immunity in alveolar macrophagesPreprint
Wang, T., Zhang, J., Miao, Y., Wang, Y., Li, Y., Wang, L., Liu, Y., Chang, C., Chen, E., & Yao, Y. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.06.14.659659
Lung macrophages in pulmonary homeostasis and disease: from basic biology to clinical applications
Zhang, Y., Sun, S., Yang, J., Chen, H., Liu, M., & Wu, H. (2025) · Central European Journal of Immunology · doi:10.5114/ceji.2025.154651
Identification Of Protein Cargo in Extracellular Vesicles from Macrophages in Progressing and Regressing TumorsPreprint
Zhu, Y., Cai, J., Suhandynata, R. T., & Bui, J. D. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.11.05.685429
Attenuation of canine distemper virus leads to a potent antiviral innate immune response with restricted infection of alveolar macrophages
Pöpperl, P., Chludzinski, E., Stoff, M., Geffers, R., Ludlow, M., & Beineke, A. (2025) · Journal of Virology · doi:10.1128/jvi.01761-25
ILC2s govern imprinting of alveolar macrophage-mediated immunity upon secondary helminth infection in the lungPreprint
Pollock, J., Patel, J. H., Graf, L.-M., Ruhl, A., Radtke, D., & Voehringer, D. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2025.12.22.695921
Chronic alcohol exposure drives inflammaging and transposon derepression in hematopoietic stem progenitor cellsPreprint
Yuda, R. A. A., Bea, H., Kellett, V., Kim, J.-W., Yang, F., Choijilsuren, H. B., Park, Y., Fu, Y., Ha, Z., Choi, J., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2025.12.25.696246 · Cited by 1
Self-citation
Biological Rhythms in Coronavirus Pneumonia Pathogenesis
Mok, H., Gierasch, C. M., Ostendorf, E., Jaiswal, A., Adler, A. J., Ganninger, A., Wright, L., Ganninger, A., Fox, C., Hyrc, K. L., et al. (2026) · Journal of Biological Rhythms · doi:10.1177/07487304261449284 · Cited by 1
Homeostatic and inflammatory functions of pulmonary GM-CSF
Lechner, A., Gschwend, J., & Schneider, C. (2026) · Mucosal Immunology · doi:10.1016/j.mucimm.2026.100380
Viral spillover among diverse animals in the water environment: Risks, mechanisms, and prevention
Zhang, Q.-Y., Ke, F., & Gui, J. (2026) · The Innovation Water · doi:10.59717/j.tiw.2026.100014
Inflammasome Activation and IL-1β Release in Alveolar Macrophages Infected with Pseudomonas aeruginosa is Reduced in HypoxiaPreprint
Rapp, A. W., Golden, A., Reuven, A. D., Goddard, J., McClure, E. A., Wilson, A., Young, C., Murphy, C., Morgan, T.-L., Olive, A. J., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.09.14.750667 · Cited by 1
Precision deployment of cell and cell-derived therapeutics across immune states and lung-injury endotypes in severe pneumonia
Li, S., Sheng, S., Fu, Z., Zhou, A., & Ning, X. (2026) · Chemical Engineering Journal · doi:10.1016/j.cej.2026.182348

Authors: Subramanian, S., Busch, C. J.-L., Molawi, K., Geirsdottir, L., Maurizio, J., Vargas Aguilar, S., Belahbib, H., Gimenez, G., Yuda, R. A. A., Burkon, M., Favret, J., Gholamhosseinian Najjar, S., de Laval, B., Kandalla, P. K., Sarrazin, S., Alexopoulou, L., & Sieweke, M. H.
Affiliations: Centre National de la Recherche Scientifique (FR)
Center for Regenerative Therapies Dresden (DE)
Inovarion (France) (FR)
Corresponding author: Sieweke, Michael H.
ORCID: Subramanian, Sethuraman
Busch, Clara Jana-Lui
Molawi, Kaaweh
Maurizio, Julien
Yuda, Ridzky Anis Advent
Gholamhosseinian Najjar, Sara
Kandalla, Prashanth Kumar
Sarrazin, Sandrine
Alexopoulou, Lena
Sieweke, Michael H.
Venue: Nature Immunology
Publisher: Springer Science and Business Media LLC
Published: 2022-02-24
Volume: 23
Issue: 3
Pages: 458-468
ISSN: 1529-2908 (ISSN-L)
1529-2916
DOI: 10.1038/s41590-022-01146-w
OpenAlex: W4213453160
Cited by: 103
References: 57
Citations by year: 2026: 23 · 2025: 29 · 2024: 22 · 2023: 21 · 2022: 8
FWCI: 7.66
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.springernature.com/gp/researchers/text-and-data-mining (tdm)
www.springernature.com/gp/researchers/text-and-data-mining (vor)
Locations: Nature Immunology (publishedVersion)
Research Square (acceptedVersion, cc-by)
PubMed (publishedVersion)
HAL (submittedVersion)
MDC Repository (Max-Delbrueck-Center for Molecular Medicine) (submittedVersion)
Qucosa (Saxon State and University Library Dresden) (submittedVersion, other-oa)
Funders: Aix-Marseille Université
European Commission (HORIZON2020)
Agence Nationale de la Recherche (ANR-17-CE15-0007, ANR-18-CE12-0019, ANR18, ANR-17)
Institut National de la Santé et de la Recherche Médicale
Technische Universität Dresden
Fondation ARC pour la Recherche sur le Cancer (PGA1 RF20170205515)
Centre National de la Recherche Scientifique
Zentrum für Regenerative Therapien Dresden
Stiftung Charité
H2020 European Research Council (695093)
Topics: Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Keywords: alveolar macrophages, macrophage identity, epigenetic reprogramming, chromatin accessibility, open chromatin, macrophage transplantation, macrophage proliferation, macrophage metabolism, macrophage adhesion, transcriptional programs
MeSH terms: Animals, Chromatin, Lung, Macrophages, Alveolar, Epigenesis, Genetic, Mice, Epigenomics

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Long-term culture-expanded alveolar macrophages restore their full epigenetic identity after transfer in vivo

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks