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Spatial organization of the somatosensory cortex revealed by osmFISH
Open accessAbstract
Global efforts to create a molecular census of the brain using single-cell transcriptomics are producing a large catalog of molecularly defined cell types. However, spatial information is lacking and new methods are needed to map a large number of cell type–specific markers simultaneously on large tissue areas. Here, we describe a cyclic single-molecule fluorescence in situ hybridization methodology and define the cellular organization of the somatosensory cortex. osmFISH applies automated cycles of single-molecule fluorescence in situ hybridization without barcoding to provide spatial gene expression in tissue sections at high sensitivity, accuracy and throughput.
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Metadata
- Authors
- Codeluppi, S., Borm, L. E., Zeisel, A., La Manno, G., van Lunteren, J. A., Svensson, C. I., & Linnarsson, S.
- Venue
- Nature Methods
- Published
- 2018-10-30
- Volume
- 15
- Issue
- 11
- Pages
- 932-935
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2952383043
- Cited by
- 643
- References
- 14
- FWCI
- 14.20
- Type
- journal article
- Language
- en
- Open access
- green
- Licence
- www.springer.com/tdm (tdm)
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- Codeluppi, S., Borm, L. E., Zeisel, A., La Manno, G., van Lunteren, J. A., Svensson, C. I., & Linnarsson, S. (2018). Spatial organization of the somatosensory cortex revealed by osmFISH. Nature Methods. https://doi.org/10.1038/s41592-018-0175-z
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