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Cited by 10Advanced X-ray Imaging TechniquesEnzyme Structure and FunctionProtein Structure and Dynamics

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Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs

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Konold, Patrick E., Monrroy, Leonardo, Bellisario, Alfredo, Filipe, Diogo, Adams, Patrick, Alvarez, Roberto, Bean, Richard, Bielecki, Johan, Bódizs, Szabolcs, Ducrocq, Gabriel, Grubmueller, Helmut, Kirian, Richard A., Kloos, Marco, Koliyadu, Jayanath C. P., Koua, Faisal H. M., Larkiala, Taru, Letrun, Romain, Lindsten, Fredrik, Maihöfer, Michael, Martin, Andrew V., Mészáros, Petra, Mutisya, Jennifer, Nimmrich, Amke, Okamoto, Kenta, Round, Adam, Sato, Tokushi, Valerio, Joana, Westphal, Daniel, Wollter, August, Yenupuri, Tej Varma, You, Tong, Maia, Filipe, Westenhoff, Sebastian·2024·Nature Methods
DOI 10.1038/s41592-024-02344-0PMC PMC11399097

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Abstract

Detecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining Jɑ helix unfolding of a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy to implement and widely applicable for direct observation of structural dynamics of many biochemical processes.

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Metadata

Authors
Konold, P. E., Monrroy, L., Bellisario, A., Filipe, D., Adams, P., Alvarez, R., Bean, R., Bielecki, J., Bódizs, S., Ducrocq, G., Grubmueller, H., Kirian, R. A., Kloos, M., Koliyadu, J. C. P., Koua, F. H. M., Larkiala, T., Letrun, R., Lindsten, F., Maihöfer, M., Martin, A. V., Mészáros, P., Mutisya, J., Nimmrich, A., Okamoto, K., Round, A., Sato, T., Valerio, J., Westphal, D., Wollter, A., Yenupuri, T. V., You, T., Maia, F., & Westenhoff, S.
Affiliations
  • Uppsala University (SE)
  • RMIT University (AU)
  • Arizona State University (US)
  • European X-Ray Free-Electron Laser (DE)
  • Linköping University (SE)
  • University of Gothenburg (SE)
  • Max Planck Institute for Multidisciplinary Sciences (DE)
  • University of Washington (US)
Corresponding author
  • Maia, Filipe
  • Westenhoff, Sebastian
ORCID
Venue
Nature Methods
Publisher
Springer Science and Business Media LLC
Published
2024-07-05
Volume
21
Issue
9
Pages
1608-1611
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
DOI
10.1038/s41592-024-02344-0
PMC
PMC11399097
OpenAlex
W4400362172
Cited by
10
References
1
Citations by year
2026: 5 · 2025: 4 · 2024: 1
FWCI
2.96
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Methods (publishedVersion, cc-by)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Diva portal (Dalarna University Library) (submittedVersion, other-oa)
  • KTH Publication Database DiVA (KTH Royal Institute of Technology) (submittedVersion, other-oa)
  • GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by)
  • European XFEL (submittedVersion)
Funders
  • Vetenskapsrådet
  • Deutscher Akademischer Austauschdienst
  • Bundesministerium für Bildung und Forschung
  • National Science Foundation
  • NSF | Directorate for Biological Sciences (1943448)
  • BioXFEL Science and Technology
  • Provincial Foundation for Excellent Young Talents of Colleges and Universities of Anhui Province
  • EC | Horizon 2020 Framework Programme (101004728)
Topics
  • Physical Sciences › Physics and Astronomy › Radiation › Advanced X-ray Imaging Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords
time-resolved X-ray scattering, MHz repetition rate, X-ray free-electron laser
MeSH terms
Lasers, Time Factors, X-Ray Diffraction, X-Rays

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