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Physio-biochemical and molecular mechanism underlying the enhanced heavy metal tolerance in highland barley seedlings pre-treated with low-dose gamma irradiation

Wang, Xiaojie, Ma, Ruonan, Cui, Dongjie, Cao, Qing, Shan, Zhe, Jiao, Zhen·2017·Scientific Reports
DOI 10.1038/s41598-017-14601-8
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Abstract

Heavy metal pollution, as a consequence of rapid industrialization and urbanization, poses a threat to highland barley grown in Tibet. This study investigates the effect of different doses of gamma irradiation (50–300 Gy) on the physio-biochemical and molecular mechanism of highland barley under heavy metal stress. Growth data showed that 50-Gy gamma irradiation had the maximal beneficial effects on the highland barley seedlings under lead/cadmium stress. The results of oxidative parameters demonstrated that 50-Gy gamma-irradiated seedlings had lower hydrogen peroxide and malondialdehyde contents under lead/cadmium stress compared to non-irradiated seedlings. Moreover, the activities of antioxidant enzyme and proline levels in 50-Gy gamma-irradiated seedlings were drastically higher than those in non-irradiated seedlings under lead/cadmium stress. Additionally, transmission electron microscopy results revealed that the 50-Gy gamma-irradiated seedlings exhibited improved chloroplasts ultrastructure compared with non-irradiated seedlings exposed to lead/cadmium stress. Notably, transcriptional expression analysis showed that 50-Gy gamma irradiation could significantly affect the expression of genes related to heavy metal transport and abscisic acid metabolism under lead/cadmium stress. Collectively, these results provide insights into the physio-biochemical and molecular mechanisms of low-dose-gamma-irradiation-enhanced heavy metal tolerance in highland barley seedlings, thus proposing gamma irradiation as a potential technology to mitigate heavy metal toxicity in crops.

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Metadata

Authors
Wang, X., Ma, R., Cui, D., Cao, Q., Shan, Z., & Jiao, Z.
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2017-10-27
Volume
7
Issue
1
ISSN
2045-2322
DOI
10.1038/s41598-017-14601-8
Cited by
55
References
1
Language
en
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Wang, X., Ma, R., Cui, D., Cao, Q., Shan, Z., & Jiao, Z. (2017). Physio-biochemical and molecular mechanism underlying the enhanced heavy metal tolerance in highland barley seedlings pre-treated with low-dose gamma irradiation. Scientific Reports. https://doi.org/10.1038/s41598-017-14601-8

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