Accelerated triacylglycerol production without growth inhibition by overexpression of a glycerol-3-phosphate acyltransferase in the unicellular red alga Cyanidioschyzon merolae

Fukuda, Satoshi; Hirasawa, Eri; Takemura, Tokiaki; Takahashi, Sota; Chokshi, Kaumeel; Pancha, Imran; Tanaka, Kan; Imamura, Sousuke

doi:10.1038/s41598-018-30809-8

Cited by 79Algal biology and biofuel productionLipid metabolism and biosynthesisMicrobial Metabolic Engineering and Bioproduction

Accelerated triacylglycerol production without growth inhibition by overexpression of a glycerol-3-phosphate acyltransferase in the unicellular red alga Cyanidioschyzon merolae

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Fukuda, Satoshi, Hirasawa, Eri, Takemura, Tokiaki, Takahashi, Sota, Chokshi, Kaumeel, Pancha, Imran, Tanaka, Kan, Imamura, Sousuke·2018·Scientific Reports

DOI 10.1038/s41598-018-30809-8PMC PMC6098107

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Abstract

Microalgae accumulate triacylglycerols (TAGs), a promising feedstock for biodiesel production, under unfavorable environmental or stress conditions for their growth. Our previous analyses revealed that only transcripts of CmGPAT1 and CmGPAT2, both encoding glycerol-3-phosphate acyltransferase, were increased among fatty acid and TAG synthesis genes under TAG accumulation conditions in the red alga Cyanidioschyzon merolae. In this study, to investigate the role of these proteins in TAG accumulation in C. merolae, we constructed FLAG-fused CmGPAT1 and CmGPAT2 overexpression strains. We found that CmGPAT1 overexpression resulted in marked accumulation of TAG even under normal growth conditions, with the maximum TAG productivity increased 56.1-fold compared with the control strain, without a negative impact on algal growth. The relative fatty acid composition of 18:2 in the TAGs and the sn−1/sn−3 positions were significantly increased compared with the control strain, suggesting that CmGPAT1 had a substrate preference for 18:2. Immunoblot analysis after cell fractionation and immunostaining analysis demonstrated that CmGPAT1 localizes in the endoplasmic reticulum (ER). These results indicate that the reaction catalyzed by the ER-localized CmGPAT1 is a rate-limiting step for TAG synthesis in C. merolae, and would be a potential target for improvement of TAG productivity in microalgae.

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Overexpressing Malonyl CoA-acylcarrier protein transacylase gene and glycerol-3-phosphate acyltransferases gene promoted neutral lipid accumulation in microalgae.
“Overexpressing Malonyl CoA-acylcarrier protein transacylase gene and glycerol-3-phosphate acyltransferases gene promoted neutral lipid accumulation in microalgae, too [186, 187].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [187]

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Additional file 1 of Lipid accumulation mechanism of Amphora coffeaeformis under nitrogen deprivation and its application as a feed additive in Carassius auratus aquaculture
Cui, Y., Kang, W., Zhou, X., Meng, C., & Gao, Z. (2023) · Figshare · doi:10.6084/m9.figshare.24761319
Additional file 4 of Expression of glycerol-3-phosphate acyltransferase increases non-polar lipid accumulation in Nannochloropsis oceanica
Südfeld, C., Kiyani, A., Wefelmeier, K., Wijffels, R. H., Barbosa, M. J., & D’Adamo, S. (2024) · Figshare · doi:10.6084/m9.figshare.26561821
Additional file 5 of Novel context-specific genome-scale modelling explores the potential of triacylglycerol production by Chlamydomonas reinhardtii
Yao, H., Dahal, S., & Yang, L. (2024) · Figshare · doi:10.6084/m9.figshare.26562175
Additional file 1 of Novel context-specific genome-scale modelling explores the potential of triacylglycerol production by Chlamydomonas reinhardtii
Yao, H., Dahal, S., & Yang, L. (2024) · Figshare · doi:10.6084/m9.figshare.26562163
Additional file 1 of Expression of glycerol-3-phosphate acyltransferase increases non-polar lipid accumulation in Nannochloropsis oceanica
Südfeld, C., Kiyani, A., Wefelmeier, K., Wijffels, R. H., Barbosa, M. J., & D’Adamo, S. (2024) · Figshare · doi:10.6084/m9.figshare.26561812
Additional file 3 of Expression of glycerol-3-phosphate acyltransferase increases non-polar lipid accumulation in Nannochloropsis oceanica
Südfeld, C., Kiyani, A., Wefelmeier, K., Wijffels, R. H., Barbosa, M. J., & D’Adamo, S. (2024) · Figshare · doi:10.6084/m9.figshare.26561818
Additional file 6 of Novel context-specific genome-scale modelling explores the potential of triacylglycerol production by Chlamydomonas reinhardtii
Yao, H., Dahal, S., & Yang, L. (2024) · Figshare · doi:10.6084/m9.figshare.26562178
Additional file 2 of Expression of glycerol-3-phosphate acyltransferase increases non-polar lipid accumulation in Nannochloropsis oceanica
Südfeld, C., Kiyani, A., Wefelmeier, K., Wijffels, R. H., Barbosa, M. J., & D’Adamo, S. (2024) · Figshare · doi:10.6084/m9.figshare.26561815
Additional file 4 of Novel context-specific genome-scale modelling explores the potential of triacylglycerol production by Chlamydomonas reinhardtii
Yao, H., Dahal, S., & Yang, L. (2024) · Figshare · doi:10.6084/m9.figshare.26562172
Additional file 7 of Novel context-specific genome-scale modelling explores the potential of triacylglycerol production by Chlamydomonas reinhardtii
Yao, H., Dahal, S., & Yang, L. (2024) · Figshare · doi:10.6084/m9.figshare.26562181
Additional file 3 of Novel context-specific genome-scale modelling explores the potential of triacylglycerol production by Chlamydomonas reinhardtii
Yao, H., Dahal, S., & Yang, L. (2024) · Figshare · doi:10.6084/m9.figshare.26562169
Additional file 2 of Novel context-specific genome-scale modelling explores the potential of triacylglycerol production by Chlamydomonas reinhardtii
Yao, H., Dahal, S., & Yang, L. (2024) · Figshare · doi:10.6084/m9.figshare.26562166
Additional file 5 of Expression of glycerol-3-phosphate acyltransferase increases non-polar lipid accumulation in Nannochloropsis oceanica
Südfeld, C., Kiyani, A., Wefelmeier, K., Wijffels, R. H., Barbosa, M. J., & D’Adamo, S. (2024) · Figshare · doi:10.6084/m9.figshare.26561824
Additional file 1 of Development of a stable semi-continuous lipid production system of an oleaginous Chlamydomonas sp. mutant using multi-omics profiling
Oyama, T., Kato, Y., Hidese, R., Matsuda, M., Matsutani, M., Watanabe, S., Kondo, A., & Hasunuma, T. (2022) · Figshare · doi:10.6084/m9.figshare.21151208
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Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red Alga Cyanidioschyzon merolae
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Authors: Fukuda, S., Hirasawa, E., Takemura, T., Takahashi, S., Chokshi, K., Pancha, I., Tanaka, K., & Imamura, S.
Affiliations: Tokyo Institute of Technology (JP)
Corresponding author: Imamura, Sousuke
ORCID: Fukuda, Satoshi
Takemura, Tokiaki
Takahashi, Sota
Chokshi, Kaumeel
Tanaka, Kan
Imamura, Sousuke
Venue: Scientific Reports
Publisher: Springer Science and Business Media LLC
Published: 2018-08-17
Volume: 8
Issue: 1
Pages: 12410-12410
ISSN: 2045-2322 (ISSN-L)
DOI: 10.1038/s41598-018-30809-8
PMC: PMC6098107
OpenAlex: W2887845774
Cited by: 78
References: 51
Citations by year: 2026: 2 · 2025: 4 · 2024: 16 · 2023: 14 · 2022: 15 · 2021: 14 · 2020: 6 · 2019: 6 · 2018: 1
FWCI: 1.56
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0 (tdm)
creativecommons.org/licenses/by/4.0 (vor)
Locations: Scientific Reports (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Tokyo Tech Research Repository (Tokyo Institute of Technology) (submittedVersion)
Funders: Japan Society for the Promotion of Science (16F16399, 18F18084, CREST, 17K07438, 18H02124)
Japan Science and Technology Agency (CREST)
Core Research for Evolutional Science and Technology
Topics: Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords: triglyceride accumulation, glycerol-3-phosphate acyltransferase, Cyanidioschyzon merolae, red algae, microalgal lipid production, biodiesel feedstock, fatty acid composition, endoplasmic reticulum localization, growth inhibition, gene overexpression
MeSH terms: Rhodophyta, Endoplasmic Reticulum, Fatty Acids, Glycerol-3-Phosphate O-Acyltransferase, Glycolipids, Phospholipids, Phylogeny, Triglycerides, Gene Expression

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Accelerated triacylglycerol production without growth inhibition by overexpression of a glycerol-3-phosphate acyltransferase in the unicellular red alga Cyanidioschyzon merolae

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