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Cited by 19Microbial Natural Products and BiosynthesisPlant Pathogens and Fungal DiseasesFungal and yeast genetics research

Phosphopantetheinyl transferase (Ppt)-mediated biosynthesis of lysine, but not siderophores or DHN melanin, is required for virulence of Zymoseptoria tritici on wheat

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Derbyshire, Mark C., Gohari, Amir Mirzadi, Mehrabi, Rahim, Kilaru, Sreedhar, Steinberg, Gero, Ali, Solaf, Bailey, Andy, Hammond-Kosack, Kim +2 more·2018·Scientific Reports
DOI 10.1038/s41598-018-35223-8PMC PMC6244202
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Abstract

Zymoseptoria tritici is the causal agent of Septoria tritici blotch (STB) disease of wheat. Z. tritici is an apoplastic fungal pathogen, which does not penetrate plant cells at any stage of infection, and has a long initial period of symptomless leaf colonisation. During this phase it is unclear to what extent the fungus can access host plant nutrients or communicate with plant cells. Several important primary and secondary metabolite pathways in fungi are regulated by the post-translational activator phosphopantetheinyl transferase (Ppt) which provides an essential co-factor for lysine biosynthesis and the activities of non-ribosomal peptide synthases (NRPS) and polyketide synthases (PKS). To investigate the relative importance of lysine biosynthesis, NRPS-based siderophore production and PKS-based DHN melanin biosynthesis, we generated deletion mutants of ZtPpt . The ∆ ZtPpt strains were auxotrophic for lysine and iron, non-melanised and non-pathogenic on wheat. Deletion of the three target genes likely affected by ZtPpt loss of function ( Aar - lysine; Nrps1 -siderophore and Pks1 - melanin), highlighted that lysine auxotrophy was the main contributing factor for loss of virulence, with no reduction caused by loss of siderophore production or melanisation. This reveals Ppt, and the lysine biosynthesis pathway, as potential targets for fungicides effective against Z. tritici .

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  • Melanin is non-essential for virulence in Sclerotinia sclerotiorum and Zymoseptoria tritici, suggesting that there is no clear correlation between melanin biosynthesis capacity and pathogenicity in these fungal species.

    “Additionally, melanin has been shown to be non-essential for virulence in Sclerotinia sclerotiorum and Zymoseptoria tritici, suggesting that there is no clear correlation between melanin biosynthesis capacity and pathogenicity in these fungal species [73,74].”

    From Fungal Melanin in Plant Pathogens: Complex Biosynthesis Pathways and Diverse Biological Functions (2025) · cites this paper as [74]

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Authors
Derbyshire, M. C., Gohari, A. M., Mehrabi, R., Kilaru, S., Steinberg, G., Ali, S., Bailey, A., Hammond-Kosack, K., Kema, G. H. J., & Rudd, J. J.
Affiliations
  • Curtin University (AU)
  • University of Tehran (IR)
  • Isfahan University of Technology (IR)
  • University of Exeter (GB)
  • Sulaimani Polytechnic University (IQ)
  • University of Bristol (GB)
  • Rothamsted Research (GB)
  • Wageningen University & Research (NL)
Corresponding author
  • Kema, Gert H. J.
  • Rudd, Jason J.
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2018-11-20
Volume
8
Issue
1
Pages
16536-16536
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-018-35223-8
PMC
PMC6244202
OpenAlex
W2900686646
Cited by
19
References
1
Citations by year
2026: 2 · 2025: 4 · 2024: 5 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 1
FWCI
0.78
Type
journal article
Language
en
Update notices
correction (2019-11-07)
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Socio-Environmental Systems Modeling (acceptedVersion, cc-by)
  • Rothamsted Repository (Rothamsted Repository) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • eSpace (Curtin University) (submittedVersion, cc-by)
  • Rothamsted Repository (Rothamsted Repository) (submittedVersion, cc-by)
  • Explore Bristol Research (submittedVersion, cc-by)
  • Bristol Research (University of Bristol) (submittedVersion, cc-by)
  • Data Archiving and Networked Services (DANS) (submittedVersion, cc-by)
Funders
  • Grains Research and Development Corporation (CUR00023)
  • Biotechnology and Biological Sciences Research Council (BB/N01597/1, BB/P016855/1)
Topics
  • Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Plant Pathogens and Fungal Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
Zymoseptoria tritici, Septoria tritici blotch, phosphopantetheinyl transferase, lysine biosynthesis, fungal virulence, siderophore production, nonribosomal peptide synthetase, polyketide synthase, melanization, auxotrophy, deletion mutants, fungicide targets, wheat pathogen
MeSH terms
Ascomycota, Bacterial Proteins, Fungal Proteins, Lysine, Melanins, Plant Diseases, Spores, Fungal, Virulence, Triticum, Siderophores, Transferases (Other Substituted Phosphate Groups), Plant Leaves

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