Cysteine Disulfides (Cys-ss-X) as Sensitive Plasma Biomarkers of Oxidative Stress
Open accessAbstract
We developed a high-throughput mass spectrometry-based method to simultaneously quantify numerous small-molecule thiols and disulfides in blood plasma. Application of this assay to analyze plasma from patients with known oxidative stress (sickle cell disease and sepsis) and from a patient with sickle cell disease treated with the antioxidant N-acetylcysteine suggests that cysteine disulfides, in particular protein-bound cysteine, serve as sensitive plasma biomarkers for the extent of oxidative stress and effectiveness of antioxidant treatment.
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Patients with sepsis have higher plasma cysteine disulfide levels than healthy donors.
“They used a UPLC-MS/MS approach for simultaneous quantification of thiols as well as disulfides and found higher levels of cysteine disulfides in plasma of patients with sickle-cell disease and sepsis compared to healthy donors [124].”
From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [124]
Patients with sickle-cell disease have higher plasma cysteine disulfide levels than healthy donors.
“They used a UPLC-MS/MS approach for simultaneous quantification of thiols as well as disulfides and found higher levels of cysteine disulfides in plasma of patients with sickle-cell disease and sepsis compared to healthy donors [124].”
From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [124]
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Research works printed but not linked: 14
- [1]Dalle-Donne I Rossi R Colombo R Giustarini D Milzani A Biomarkers of oxidative damage in human disease Clin. Chem. 2006 52 601 623 10.1373/clinchem.2005.061408 16484333 · DOI · Paper page
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- [3]Rossi R Dalle-Donne I Milzani A Giustarini D Oxidized forms of glutathione in peripheral blood as biomarkers of oxidative stress Clin. Chem. 2006 52 1406 1414 10.1373/clinchem.2006.067793 16690733 · DOI
- [4]Rahman I Kode A Biswas SK Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method Nat. Protoc. 2006 1 3159 3165 10.1038/nprot.2006.378 17406579 · DOI
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- [6]Winther JR Thorpe C Quantification of thiols and disulfides Biochim. Biophys. Acta. 2014 1840 838 846 10.1016/j.bbagen.2013.03.031 23567800 PMC3766385 · DOI
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- [8]Giustarini D Dalle-Donne I Milzani A Rossi R Detection of glutathione in whole blood after stabilization with N-ethylmaleimide Anal. Biochem. 2011 415 81 83 10.1016/j.ab.2011.04.013 21545786 · DOI
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- [10]Sjodin K Nilsson E Hallberg A Tunek A Metabolism of N-acetyl-L-cysteine. Some structural requirements for the deacetylation and consequences for the oral bioavailability Biochem. Pharmacol. 1989 38 3981 3985 10.1016/0006-2952(89)90677-1 2597179 · DOI
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Cited by
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Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases
Kehm, R., Baldensperger, T., Raupbach, J., & Höhn, A. (2021) · Redox Biology · doi:10.1016/j.redox.2021.101901 · Cited by 348 · see citing works
“They used a UPLC-MS/MS approach for simultaneous quantification of thiols as well as disulfides and found higher levels of cysteine disulfides in plasma of patients with sickle-cell disease and sepsis compared to healthy donors [124].”
Method
- Authors
- Fu, X., Cate, S. A., Dominguez, M., Osborn, W., Özpolat, T., Konkle, B. A., Chen, J., & López, J. A.
- Affiliations
- University of Washington (US)
- Bloodworks Northwest (US)
- Corresponding author
- Fu, Xiaoyun
- López, José A.
- Venue
- Scientific Reports
- Published
- 2019-01-14
- Volume
- 9
- Issue
- 1
- Pages
- 115-115
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC6331564
- OpenAlex
- W2909933948
- Cited by
- 59
- References
- 0
- Citations by year
- 2026: 8 · 2025: 7 · 2024: 6 · 2023: 13 · 2022: 9 · 2021: 6 · 2020: 7 · 2019: 1 · 2018: 1
- FWCI
- 1.90
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (HL112633)
- Bloodworks Research Institute, Seattle, WA
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- cysteine disulfide, oxidative stress, plasma biomarkers, disulfides, sickle cell disease, sepsis, N-acetylcysteine, antioxidant treatment
- MeSH terms
- Acetylcysteine, Anemia, Sickle Cell, Antioxidants, Cysteine, Disulfides, Humans, Biomarkers, Oxidative Stress, Sepsis
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