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Cited by 19Knee injuries and reconstruction techniquesTendon Structure and TreatmentOsteoarthritis Treatment and Mechanisms

Anterior cruciate ligament remnant cells have different potentials for cell differentiation based on their location

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Lee, Jin Kyu, Jo, Sungsin, Lee, Young Lim, Park, Hyosun, Song, Jun-Seob, Sung, Il-Hoon, Kim, Tae-Hwan·2020·Scientific Reports
DOI 10.1038/s41598-020-60047-wPMC PMC7033160
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Abstract

Histological and cytological observations of the human anterior cruciate ligament (ACL) had been described, but the differentiation potency based on their location is still unknown. To determine and compare proliferation and differentiation potential of cells derived from distal and middle thirds of the ACL remnant, ACL remnant was initially marked at the distal third (within 10 mm from the tibial insertion) and middle third (between 10–20 mm from the tibial insertion) and then dissected. Both the middle and distal third regions of ACL remnant were analyzed using CD34+ cell counting. Cell proliferation rate did not differ in both middle and distal third regions of ACL remnant, but they showed different characteristics in cell differentiation depending on their location. The distal third region of the ACL remnant had a tendency for chondrogenic differentiation with higher expression of CD34+ cells. On the other hand, the middle third region of ACL remnant had a strong tendency for osteogenic and ligamentous differentiation. Characteristics of the ACL remnant tissues should be considered when performing remnant-preserving or harvesting ACL remnants for tissue engineering.

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  1. [1]Duquin TR Wind WM Fineberg MS Smolinski RJ Buyea CM Current trends in anterior cruciate ligament reconstruction J. Knee Surg. 2009 22 7 12 10.1055/s-0030-1247719 19216345 · DOI
  2. [3]Agung M Mobilization of bone marrow-derived mesenchymal stem cells into the injured tissues after intraarticular injection and their contribution to tissue regeneration Knee Surg. Sports Traumatol. Arthrosc. 2006 14 1307 1314 10.1007/s00167-006-0124-8 16788809 · DOI
  3. [4]Holm I Oiestad BE Risberg MA Gunderson R Aune AK No differences in prevalence of osteoarthritis or function after open versus endoscopic technique for anterior cruciate ligament reconstruction: 12-year follow-up report of a randomized controlled trial Am. J. Sports Med. 2012 40 2492 2498 10.1177/0363546512458766 22984128 · DOI
  4. [5]Lee DW Shim JC Yang SJ Cho SI Kim JG Functional Effects of Single Semitendinosus Tendon Harvesting in Anatomic Anterior Cruciate Ligament Reconstruction: Comparison of Single versus Dual Hamstring Harvesting Clin. Orthop. Surg. 2019 11 60 72 10.4055/cios.2019.11.1.60 30838109 PMC6389538 · DOI
  5. [6]Kyung, H.-S. Graft considerations for successful anterior cruciate ligament reconstruction. Knee Surgery & Related Research31, 10.1186/s43019-019-0003-5 (2019). PMC7219513 32660624 · DOI
  6. [7]Ballock RT Woo SL Lyon RM Hollis JM Akeson WH Use of patellar tendon autograft for anterior cruciate ligament reconstruction in the rabbit: a long-term histologic and biomechanical study J. Orthop. Res. 1989 7 474 485 10.1002/jor.1100070404 2738766 · DOI · Paper page
  7. [8]Mehta A Effects of Anteromedial Portal versus Transtibial ACL Tunnel Preparation on Contact Characteristics of the Graft and the Tibial Tunnel Aperture Clin. Orthop. Surg. 2019 11 52 59 10.4055/cios.2019.11.1.52 30838108 PMC6389531 · DOI
  8. [9]Grana WA Egle DM Mahnken R Goodhart CW An analysis of autograft fixation after anterior cruciate ligament reconstruction in a rabbit model Am. J. Sports Med. 1994 22 344 351 10.1177/036354659402200309 8037275 · DOI
  9. [10]Tohyama H Yoshikawa T Ju YJ Yasuda K Revascularization in the tendon graft following anterior cruciate ligament reconstruction of the knee: its mechanisms and regulation Chang. Gung Med. J. 2009 32 133 139 19403002
  10. [11]Cobellis G Beneficial effects of VEGF secreted from stromal cells in supporting endothelial cell functions: therapeutic implications for critical limb ischemia Cell Transpl. 2010 19 1425 1437 10.3727/096368910X509068 20587143 · DOI
  11. [12]Kalka C Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization Proc. Natl Acad. Sci. USA 2000 97 3422 3427 10.1073/pnas.070046397 10725398 PMC16255 · DOI
  12. [13]Kawamoto A Intramyocardial transplantation of autologous endothelial progenitor cells for therapeutic neovascularization of myocardial ischemia Circulation 2003 107 461 468 10.1161/01.cir.0000046450.89986.50 12551872 · DOI
  13. [14]Mifune Y Tendon graft revitalization using adult anterior cruciate ligament (ACL)-derived CD34+ cell sheets for ACL reconstruction Biomater. 2013 34 5476 5487 10.1016/j.biomaterials.2013.04.013 23632324 · DOI
  14. [15]Mifune Y Therapeutic potential of anterior cruciate ligament-derived stem cells for anterior cruciate ligament reconstruction Cell Transpl. 2012 21 1651 1665 10.3727/096368912X647234 22732227 · DOI · Paper page
  15. [16]Matsumoto T Reduction of tunnel enlargement with use of autologous ruptured tissue in anterior cruciate ligament reconstruction: a pilot clinical trial Arthrosc. 2014 30 468 474 10.1016/j.arthro.2013.12.014 24582053 · DOI · Paper page
  16. [17]Matsumoto T Acceleration of tendon-bone healing of anterior cruciate ligament graft using autologous ruptured tissue Am. J. Sports Med. 2012 40 1296 1302 10.1177/0363546512439026 22427618 · DOI
  17. [18]Ahn GY Factors Affecting the Extent of Graft Tendon Synovialization after Double-Bundle Anterior Cruciate Ligament Reconstruction: Based on Second-Look Arthroscopic Findings Clin. Orthop. Surg. 2018 10 413 419 10.4055/cios.2018.10.4.413 30505408 PMC6250959 · DOI
  18. [19]Petersen W Tillmann B Structure and vascularization of the cruciate ligaments of the human knee joint Anat. Embryol. 1999 200 325 334 10.1007/s004290050283 10463347 · DOI
  19. [20]Ahn JH Anterior cruciate ligament reconstruction using remnant preservation and a femoral tensioning technique: clinical and magnetic resonance imaging results Arthrosc. 2011 27 1079 1089 10.1016/j.arthro.2011.03.002 21704468 · DOI
  20. [21]Dhillon MS Bali K Vasistha RK Immunohistological evaluation of proprioceptive potential of the residual stump of injured anterior cruciate ligaments (ACL) Int. Orthop. 2010 34 737 741 10.1007/s00264-009-0948-1 20135121 PMC2903166 · DOI
  21. [22]Sun L Zhou X Wu B Tian M Inhibitory effect of synovial fluid on tendon-to-bone healing: an experimental study in rabbits Arthrosc. 2012 28 1297 1305 10.1016/j.arthro.2012.02.017 22607829 · DOI
  22. [23]Bray RC Leonard CA Salo PT Vascular physiology and long-term healing of partial ligament tears J. Orthop. Res. 2002 20 984 989 10.1016/S0736-0266(02)00012-8 12382963 · DOI
  23. [24]Kodama Y Furumatsu T Hino T Kamatsuki Y Ozaki T Minimal Ablation of the Tibial Stump Using Bony Landmarks Improved Stability and Synovial Coverage Following Double-Bundle Anterior Cruciate Ligament Reconstruction Knee Surg. Relat. Res. 2018 30 348 355 10.5792/ksrr.18.024 30466255 PMC6254876 · DOI
  24. [25]Zhang S Anterior cruciate ligament remnant tissue harvested within 3-months after injury predicts higher healing potential BMC Musculoskelet. Disord. 2015 16 390 10.1186/s12891-015-0855-0 26687109 PMC4684911 · DOI
  25. [26]Takazawa Y ACL Reconstruction Preserving the ACL Remnant Achieves Good Clinical Outcomes and Can Reduce Subsequent Graft Rupture Orthop. J. Sports Med. 2013 1 2325967113505076 10.1177/2325967113505076 26535246 PMC4555494 · DOI
  26. [27]Kim MK Comparison of second-look arthroscopic findings and clinical results according to the amount of preserved remnant in anterior cruciate ligament reconstruction Knee 2014 21 774 778 10.1016/j.knee.2014.02.011 24704171 · DOI
  27. [28]Guo L An Arthroscopic Second-Look Study on the Effect of Remnant Preservation on Synovialization of Bone-Patellar Tendon-Bone Allograft in Anterior Cruciate Ligament Reconstruction Arthrosc. 2016 32 868 877 10.1016/j.arthro.2015.11.017 26821962 · DOI
  28. [29]Noh JH Kyung HS Roh YH Kang TS Remnant-preserving and re-tensioning technique to cover the graft in anterior cruciate ligament reconstruction Knee Surg. Sports Traumatol. Arthrosc. 2017 25 1205 1210 10.1007/s00167-015-3854-7 26564212 · DOI
  29. [30]Cole MA Quan T Voorhees JJ Fisher GJ Extracellular matrix regulation of fibroblast function: redefining our perspective on skin aging J. Cell Commun. Signal. 2018 12 35 43 10.1007/s12079-018-0459-1 29455303 PMC5842211 · DOI · Paper page
  30. [31]Nakano N Age-dependent healing potential of anterior cruciate ligament remnant-derived cells Am. J. Sports Med. 2015 43 700 708 10.1177/0363546514561436 25556219 · DOI
  31. [32]Naraoka T Time-dependent gene expression and immunohistochemical analysis of the injured anterior cruciate ligament Bone Jt. Res. 2012 1 238 244 10.1302/2046-3758.110.2000118 PMC3626253 23610654 · DOI
  32. [33]Kirizuki S The Influence of Ruptured Scar Pattern on the Healing Potential of Anterior Cruciate Ligament Remnant Cells Am. J. Sports Med. 2018 46 1382 1388 10.1177/0363546518755753 29505728 · DOI · Paper page
  33. [34]Prager P Mesenchymal Stem Cells Isolated from the Anterior Cruciate Ligament: Characterization and Comparison of Cells from Young and Old Donors Knee Surg. Relat. Res. 2018 30 193 205 10.5792/ksrr.17.067 29554721 PMC6122945 · DOI
  34. [35]Duthon VB Anatomy of the anterior cruciate ligament Knee Surg. Sports Traumatol. Arthrosc. 2006 14 204 213 10.1007/s00167-005-0679-9 16235056 · DOI
  35. [36]Jo S Identification and characterization of human bone-derived cells Biochem. Biophys. Res. Commun. 2018 495 1257 1263 10.1016/j.bbrc.2017.11.155 29180008 · DOI
  36. [37]Herlofsen SR Kuchler AM Melvik JE Brinchmann JE Chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells in self-gelling alginate discs reveals novel chondrogenic signature gene clusters Tissue Eng. Part. A 2011 17 1003 1013 10.1089/ten.TEA.2010.0499 21087199 PMC3063696 · DOI
  37. [38]Hildebrand KA The effects of platelet-derived growth factor-BB on healing of the rabbit medial collateral ligament. An in vivo study Am. J. Sports Med. 1998 26 549 554 10.1177/03635465980260041401 9689377 · DOI
  38. [39]Batten ML Hansen JC Dahners LE Influence of dosage and timing of application of platelet-derived growth factor on early healing of the rat medial collateral ligament J. Orthop. Res. 1996 14 736 741 10.1002/jor.1100140509 8893766 · DOI
  39. [40]Jo S IL-17A induces osteoblast differentiation by activating JAK2/STAT3 in ankylosing spondylitis Arthritis Res. Ther. 2018 20 115 10.1186/s13075-018-1582-3 29880011 PMC5992730 · DOI

Cited by

Sorted by citations

Authors
Lee, J. K., Jo, S., Lee, Y. L., Park, H., Song, J.-S., Sung, I.-H., & Kim, T.-H.
Affiliations
  • Hanyang University Seoul Hospital (KR)
  • Hanyang University (KR)
Corresponding author
Kim, Tae-Hwan
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2020-02-20
Volume
10
Issue
1
Pages
3097-3097
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-020-60047-w
PMC
PMC7033160
OpenAlex
W3008413096
Cited by
19
References
1
Citations by year
2026: 3 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 7 · 2021: 3
FWCI
2.74
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • National Research Foundation (2019R1A2C2004214)
  • Korea Health Industry Development Institute (HI17C0888)
  • National Research Foundation of Korea (2016R1A2B4008606, 2019R1A2C2004214, NRF-2016R1A2B4008606, 2017R1A6A3A11034394)
Topics
  • Health Sciences › Medicine › Surgery › Knee injuries and reconstruction techniques
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Tendon Structure and Treatment
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
Keywords
differentiation potential, chondrogenic differentiation, osteogenic differentiation, CD34+ cells, cell proliferation, tissue engineering
MeSH terms
Anterior Cruciate Ligament Injuries, Adolescent, Adult, Cell Differentiation, Female, Flow Cytometry, Humans, Male, Osteogenesis, Stem Cells, Anterior Cruciate Ligament, Antigens, CD34, Chondrogenesis, Cell Proliferation, Young Adult

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