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Green Synthesis of Zinc Oxide Nanoparticles Using Aqueous Extract of Deverra tortuosa and their Cytotoxic Activities

Selim, Yasser A., Azb, Maha A., Ragab, Islam, H. M. Abd El-Azim, Mohamed·2020·Scientific Reports
DOI 10.1038/s41598-020-60541-1PMC PMC7044426
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Abstract

In recent years, there is a growing interest towards the green synthesis of metal nanoparticles, particularly from plants; however, yet no published study on the synthesis of ZnO.NPs using the Deverra tortuosa extract. Through this study, zinc oxide nanoparticles (ZnO.NPs) have been synthesized based on using the environmentally benign extract of the aerial parts of D. tortuosa as a reducing and capping agent. ZnO.NPs synthesis was confirmed using UV-Visible (UV-Vis) spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD) and High Resolution-Transmission Electron Microscope (HR-TEM). The qualitative and quantitative analyses of plant extract were done. The potential anticancer activity was in vitro investigated against two cancer cell lines (human colon adenocarcinoma “Caco-2” and human lung adenocarcinoma “A549”) compared to their activities on the human lung fibroblast cell line (WI38) using the MTT assay. Both the aqueous extract and ZnO.NPs showed a remarkable selective cytotoxicity against the two examined cancer cell lines.

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Crossref · 10.1038/s41598-020-60541-1

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Figure 1 HPLC chromatogram: (A) Standard mixture of polyphenolic compounds; (B) Aqueous extract of D. tortuosa aerial parts.
Figure 1 HPLC chromatogram: (A) Standard mixture of polyphenolic compounds; (B) Aqueous extract of D. tortuosa aerial parts.CC BY · PubMed Central
Figure 2 Chemical structures of polyphenolic compounds of D. tortuosa aerial parts.
Figure 2 Chemical structures of polyphenolic compounds of D. tortuosa aerial parts.CC BY · PubMed Central
Figure 3 X-ray diffraction pattern of ZnO.NPs.
Figure 3 X-ray diffraction pattern of ZnO.NPs.CC BY · PubMed Central
Figure 4 HR-TEM micrograph of ZnO.NPs.
Figure 4 HR-TEM micrograph of ZnO.NPs.CC BY · PubMed Central
Figure 5 IC50 of D. tortuosa aqueous extract, ZnO.NPs and Doxorubicin on the Caco-2 and A549 cancer cells compared to WI38 normal cells.
Figure 5 IC50 of D. tortuosa aqueous extract, ZnO.NPs and Doxorubicin on the Caco-2 and A549 cancer cells compared to WI38 normal cells.CC BY · PubMed Central

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Authors
Selim, Y. A., Azb, M. A., Ragab, I., & H. M. Abd El-Azim, M.
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2020-02-26
Volume
10
Issue
1
ISSN
2045-2322
DOI
10.1038/s41598-020-60541-1
PMC
PMC7044426
Cited by
552
References
0
Language
en
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