The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Comparison between 16S rRNA and shotgun sequencing data for the taxonomic characterization of the gut microbiota
Open accessAbstract
In this paper we compared taxonomic results obtained by metataxonomics (16S rRNA gene sequencing) and metagenomics (whole shotgun metagenomic sequencing) to investigate their reliability for bacteria profiling, studying the chicken gut as a model system. The experimental conditions included two compartments of gastrointestinal tracts and two sampling times. We compared the relative abundance distributions obtained with the two sequencing strategies and then tested their capability to distinguish the experimental conditions. The results showed that 16S rRNA gene sequencing detects only part of the gut microbiota community revealed by shotgun sequencing. Specifically, when a sufficient number of reads is available, Shotgun sequencing has more power to identify less abundant taxa than 16S sequencing. Finally, we showed that the less abundant genera detected only by shotgun sequencing are biologically meaningful, being able to discriminate between the experimental conditions as much as the more abundant genera detected by both sequencing strategies.
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Cites
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Cited by
McGuinness, A. J., Davis, J. A., Dawson, S. L., Loughman, A., Collier, F., O’Hely, M., Simpson, C. A., Green, J., Marx, W., Hair, C., et al. (2022) · Molecular Psychiatry · doi:10.1038/s41380-022-01456-3 · Cited by 502
Targeting the gut and tumor microbiota in cancer
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Next-generation sequencing: insights to advance clinical investigations of the microbiome
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Yasir, M., Al-Zahrani, I. A., Bibi, F., Abd El Ghany, M., & Azhar, E. I. (2022) · Food Research International · doi:10.1016/j.foodres.2022.111190 · Cited by 60
Gut microbiome and amyotrophic lateral sclerosis: a systematic review of current evidence
Sun, J., Huang, T., Debelius, J. W., & Fang, F. (2021) · Journal of Internal Medicine · doi:10.1111/joim.13336 · Cited by 43
Metadata
- Authors
- Durazzi, F., Sala, C., Castellani, G., Manfreda, G., Remondini, D., & De Cesare, A.
- Venue
- Scientific Reports
- Published
- 2021-02-04
- Volume
- 11
- Issue
- 1
- Pages
- 3030-3030
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W3128701120
- Cited by
- 594
- References
- 36
- FWCI
- 26.63
- Type
- journal article
- Language
- en
- Open access
- gold
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- Durazzi, F., Sala, C., Castellani, G., Manfreda, G., Remondini, D., & De Cesare, A. (2021). Comparison between 16S rRNA and shotgun sequencing data for the taxonomic characterization of the gut microbiota. Scientific Reports. https://doi.org/10.1038/s41598-021-82726-y
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