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Cited by 31Plant tissue culture and regenerationFlowering Plant Growth and CultivationGrowth and nutrition in plants

Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

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Sultana, Kaniz Wahida, Das, Sumanta, Chandra, Indrani, Roy, Anindita·2022·Scientific Reports
DOI 10.1038/s41598-022-05787-7PMC PMC8803877
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Abstract

Thunbergia coccinea Wall. ex D. Don being a rare, ornamental and medicinal plant of India, is needed to propagate for conserving the germplasm and analyzing its phytochemical compounds in the future. A reliable protocol for direct in vitro propagation using nodal shoot meristem of T. coccinea as explant was standardized. The highest number of shoots per explant (22.17 ± 0.54) with maximum shoot length (2.36 ± 0.28) in cm was obtained in Murashige and Skoog (MS) medium supplemented with 9.70 µM of 6-furfurylaminopurine (Kinetin) and 0.053 µM of α-naphthaleneacetic acid (NAA) combination, among all the different plant growth regulators (PGR’s) and concentrations tested. The aforesaid PGR’s combination was optimum for axillary shoot bud induction and multiplication in T. coccinea. The best rooting was observed on the half-strength MS medium fortified with 2.68 µM NAA with the highest number of roots per shoot (3.75 ± 0.12) and maximum length (5.22 ± 0.32) in cm. All the in vitro raised plantlets were acclimatized in sterile sand and soil mixture (1:1) with a survival rate of 70% on earthen pots under greenhouse conditions. PCR-based RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeat) molecular markers were employed to determine the genetic homogeneity amongst the plantlets. Twelve (12) RAPD and nine (9) ISSR primers developed a total of 104 and 91 scorable bands, respectively. The band profiles of micropropagated plantlets were monomorphic to the mother, donor in vivo plant, and similarity values varied from 0.9542–1.000. The dendrogram generated through UPGMA (unweighted pair group method with arithmetic mean) showed 99% similarities amongst all tested plants confirming the genetic uniformity of in vitro raised plants.

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  • In studies using a combination of two or three types of markers (SCoT, ISSR, RAPD), research revealed genetic fidelity of regenerated plantlets, even for those that had passed through the callus stage.

    “There are a number of studies in which the somaclonal variation analysis is carried out using a combination of two or three types of markers (Table 2), such as SCot, ISSR, and RAPD, and, in all cases, the research revealed the genetic fidelity of the regenerated plantlets (even for those that had passed through the callus stage) [73,74,75,76,77,78].”

    From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [74]

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Research works printed but not linked: 25

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Authors
Sultana, K. W., Das, S., Chandra, I., & Roy, A.
Affiliations
University of Burdwan (IN)
Corresponding author
Chandra, Indrani
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2022-01-31
Volume
12
Issue
1
Pages
1683-1683
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-022-05787-7
PMC
PMC8803877
OpenAlex
W4210400351
Cited by
31
References
0
Citations by year
2026: 5 · 2025: 7 · 2024: 3 · 2023: 10 · 2022: 6
FWCI
2.12
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
University of Burdwan
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Growth and nutrition in plants
Keywords
Thunbergia coccinea, micropropagation, shoot multiplication, plant growth regulators, kinetin, naphthaleneacetic acid, rooting, acclimatization, genetic homogeneity, RAPD markers, ISSR markers, germplasm conservation
MeSH terms
Genetic Markers, Genotype, Kinetin, Naphthaleneacetic Acids, Plant Growth Regulators, Genes, Plant, Gene Expression Regulation, Plant, Meristem, DNA, Plant, Genome, Plant, Microsatellite Repeats, Random Amplified Polymorphic DNA Technique, Acanthaceae, Genomic Instability

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