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Cited by 13Advanced Neuroimaging Techniques and ApplicationsAdvanced MRI Techniques and ApplicationsMultiple Sclerosis Research Studies

High B-value diffusion tensor imaging for early detection of hippocampal microstructural alteration in a mouse model of multiple sclerosis

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Crombé, Amandine, Nicolas, Renaud, Richard, Nathalie, Tourdias, Thomas, Hiba, Bassem·2022·Scientific Reports
DOI 10.1038/s41598-022-15511-0PMC PMC9283448
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Abstract

Several studies have highlighted the value of diffusion tensor imaging (DTI) with strong diffusion weighting to reveal white matter microstructural lesions, but data in gray matter (GM) remains scarce. Herein, the effects of b-values combined with different numbers of diffusion-encoding directions (NDIRs) on DTI metrics to capture the normal hippocampal microstructure and its early alterations were investigated in a mouse model of multiple sclerosis (experimental autoimmune encephalomyelitis [EAE]). Two initial DTI datasets (B2700-43Dir acquired with b = 2700 s.mm −2 and NDIR = 43; B1000-22Dir acquired with b = 1000 s.mm −2 and NDIR = 22) were collected from 18 normal and 18 EAE mice at 4.7 T. Three additional datasets (B2700-22Dir, B2700-12Dir and B1000-12Dir) were extracted from the initial datasets. In healthy mice, we found a significant influence of b-values and NDIR on all DTI metrics. Confronting unsupervised hippocampal layers classification to the true anatomical classification highlighted the remarkable discrimination of the molecular layer with B2700-43Dir compared with the other datasets. Only DTI from the B2700 datasets captured the dendritic loss occurring in the molecular layer of EAE mice. Our findings stress the needs for both high b-values and sufficient NDIR to achieve a GM DTI with more biologically meaningful correlations, though DTI-metrics should be interpreted with caution in these settings.

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  • The right-left dissection replicability provides sufficient precision to detect much smaller volume differences than in previous work.

    “This level of right-left dissection replicability provides sufficient precision to detect much smaller volume differences than in previous work33.”

    From Magnetic resonance microscopy maps widespread effects of Alzheimer’s disease on brain structures and behavior in mice (2026) · cites this paper as [33]

    The Materials and methods (Data analysis; page unavailable) describes delineating hippocampal regions on MRI images, not right-left physical dissection. The Results (Quality control—SNR and CNR; page unavailable) discusses image quality, but our copy provides no right-left dissection replicability measure or comparison of detectable volume differences with previous work. Searches for those terms found no supporting passage. (LLM, conf=0.76)

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Authors
Crombé, A., Nicolas, R., Richard, N., Tourdias, T., & Hiba, B.
Affiliations
  • Université de Bordeaux (FR)
  • Centre National de la Recherche Scientifique (FR)
  • Lyon 1 Université (FR)
Corresponding author
Hiba, Bassem
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2022-07-14
Volume
12
Issue
1
Pages
12008-12008
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-022-15511-0
PMC
PMC9283448
OpenAlex
W4285390377
Cited by
13
References
45
Citations by year
2026: 1 · 2025: 5 · 2024: 3 · 2023: 4
FWCI
1.71
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Oskar-Bordeaux (Universite de Bordeaux) (submittedVersion)
  • HAL (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Agence Nationale de la Recherche
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced Neuroimaging Techniques and Applications
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
Keywords
diffusion tensor imaging, high b-value, hippocampus, gray matter microstructure, multiple sclerosis, experimental autoimmune encephalomyelitis, molecular layer
MeSH terms
Animals, Disease Models, Animal, Encephalomyelitis, Autoimmune, Experimental, Hippocampus, Multiple Sclerosis, Diffusion Magnetic Resonance Imaging, Mice, Diffusion Tensor Imaging, White Matter, Gray Matter

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